Respiratory and skin hypersensitivity reactions caused by a peptide coupling reagent

Vandenplas, Olivier; Hereng, M.; Heymans, Julie; Huaux, François; Lilet-Leclercq, Chantal; Dezfoulian, Bita; Grand, J.L.; Thimpont, Joël

doi:10.1136/oem.2008.039123

Cited by 8 publications

(4 citation statements)

References 5 publications

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“…The authors confirmed the researchers’ sensitivity to HBTU through patch testing and found he was not allergic to dimethylformamide, dichloromethane, acetonitrile, triisopropylsilane, HATU, or BOP . From 2006 to 2010, six more instances of chemical sensitization from HBTU were reported with similar respiratory and skin reactions. − One example, in 2006, involved a university researcher developing an anaphylactic response to HBTU over the course of three years, similar to the case reported in this paper . All of these examples were published in allergy and other medical journals, which are not generally read by researchers who use peptide coupling agents.…”

Section: Literature Searchsupporting

confidence: 65%

Anaphylaxis Induced by Peptide Coupling Agents: Lessons Learned from Repeated Exposure to HATU, HBTU, and HCTU

2019

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Section: Literature Searchsupporting

confidence: 65%

Anaphylaxis Induced by Peptide Coupling Agents: Lessons Learned from Repeated Exposure to HATU, HBTU, and HCTU

2019

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“…Among chemicals, eight (8) new agents inducing OA have been reported for the period [43–50] (Table 1). Among these, Vandenplas et al documented a case of OA and rhinitis due to uronium salt, a compound used as a peptide coupling agent [43], ascertained by an early asthmatic reaction during the SIC and positive SPT.…”

Section: Resultsmentioning

confidence: 99%

“…Among these, Vandenplas et al documented a case of OA and rhinitis due to uronium salt, a compound used as a peptide coupling agent [43], ascertained by an early asthmatic reaction during the SIC and positive SPT. They also reported three (3) other workers with skin symptoms and rhinitis, demonstrating positive SPT.…”

Section: Resultsmentioning

confidence: 99%

Occupational Asthma: New Low-Molecular-Weight Causal Agents, 2000–2010

et al. 2012

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Background. More than 400 agents have been documented as causing occupational asthma (OA). The list of low-molecular-weight (LMW) agents that have been identified as potential causes of OA is constantly expanding, emphasizing the need to continually update our knowledge by reviewing the literature. Objective. The objective of this paper was to identify all new LMW agents causing occupational asthma reported during the period 2000–2010. Methods. A Medline search was performed using the keywords occupational asthma, new allergens, new causes, and low-molecular-weight agents. Results. We found 39 publications describing 41 new LMW causal agents, which belonged to the following categories: drugs (n = 12), wood dust (n = 11), chemicals (n = 8), metals (n = 4), biocides (n = 3), and miscellaneous (n = 3). The diagnosis of OA was confirmed through SIC for 35 of 41 agents, peak expiratory flow monitoring for three (3) agents, and the clinical history alone for three (3) agents. Immunological tests provided evidence supporting an IgE-mediated mechanism for eight (8) (20%) of the newly described agents. Conclusion. This paper highlights the importance of being alert to the occurrence of new LMW sensitizers, which can elicit OA. The immunological mechanism is explained by a type I hypersensitivity reaction in 20% of all newly described LMW agents.

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“…Moreover, we showed that the uronium salts HBTU and TBTU elicited greater expression of CD54 on the THP-1 cells in coculture, as compared to monoculture. HBTU and TBTU are both respiratory sensitisers and are used as coupling reagents in peptide synthesis; 43 they reportedly did not elicit positive skin sensitisation responses in the h-CLAT. 37 In agreement with these findings, after THP-1 cell monocultures were exposed to HBTU or TBTU, the mean RFI for CD54 was below the threshold for a positive response (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells

Tanabe,

Yoshida,

Ishikawa

et al. 2023

Altern Lab Anim

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Chemical respiratory sensitisation is a serious health problem. However, to date, there are no validated test methods available for identifying respiratory sensitisers. The aim of this study was to develop an in vitro sensitisation test by modifying the human cell line activation test (h-CLAT) to detect respiratory sensitisers and distinguish them from skin sensitisers. THP-1 cells were exposed to the test chemicals (two skin sensitisers and six respiratory sensitisers), either as monocultures or as cocultures with air–liquid interface-cultured reconstructed human bronchial epithelium. The responses were analysed by measuring the expression levels of surface markers on THP-1 cells (CD86, CD54 and OX40L) and the concentrations of cytokines in the culture media (interleukin (IL)-8, IL-33 and thymic stromal lymphopoietin (TSLP)). The cocultures exhibited increased CD54 expression on THP-1 cells; moreover, in the cocultures but not in the monocultures, exposure to two uronium salts (i.e. respiratory sensitisers) increased CD54 expression on THP-1 cells to levels above the criteria for a positive h-CLAT result. Additionally, exposure to the respiratory sensitiser abietic acid, significantly increased IL-8 concentration in the culture medium, but only in the cocultures. Although further optimisation of the method is needed to distinguish respiratory from skin sensitisers by using these potential markers (OX40L, IL-33 and TSLP), the coculture of THP-1 cells with bronchial epithelial cells offers a potentially useful approach for the detection of respiratory sensitisers.

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Respiratory and skin hypersensitivity reactions caused by a peptide coupling reagent

Cited by 8 publications

References 5 publications

Anaphylaxis Induced by Peptide Coupling Agents: Lessons Learned from Repeated Exposure to HATU, HBTU, and HCTU

Anaphylaxis Induced by Peptide Coupling Agents: Lessons Learned from Repeated Exposure to HATU, HBTU, and HCTU

Occupational Asthma: New Low-Molecular-Weight Causal Agents, 2000–2010

Development of an In Vitro Sensitisation Test Using a Coculture System of Human Bronchial Epithelium and Immune Cells

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