Respiratory Deficiency Mediates the Regulation of CHO1-encoded Phosphatidylserine Synthase by mRNA Stability in Saccharomyces cerevisiae

Choi, Hyeon‐Son; Carman, George M.

doi:10.1074/jbc.m705098200

Cited by 9 publications

(14 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In S. cerevisiae, phospholipid synthesis is a complex process that is regulated by both genetic and biochemical mechanisms (1,(3)(4)(5)(6)(7)(8)(9). The expression of phospholipid synthesis genes is controlled at the levels of transcription and mRNA stability (1,3,10). The activities of key phospholipid synthesis enzymes are regulated by lipids, water-soluble phospholipid precursors and products, and by the covalent modification of phosphorylation (1,4,8).…”

mentioning

confidence: 99%

Regulation of phospholipid synthesis in yeast

Carman

Han

2009

Journal of Lipid Research

Self Cite

102

109

View full text Add to dashboard Cite

Phospholipid synthesis in the yeast Saccharomyces cerevisiae is a complex process that involves regulation by both genetic and biochemical mechanisms. The activity levels of phospholipid synthesis enzymes are controlled by gene expression (e.g., transcription) and by factors (lipids, water-soluble phospholipid precursors and products, and covalent modification of phosphorylation) that modulate catalysis. Phosphatidic acid, whose levels are controlled by the biochemical regulation of key phospholipid synthesis enzymes, plays a central role in the regulation of phospholipid synthesis gene expression.-Carman, G. M., and G-S. Han. Regulation of phospholipid synthesis in yeast. J. Lipid Res. 2009. 50: S69-S73.

show abstract

mentioning

confidence: 99%

Regulation of phospholipid synthesis in yeast

Carman

Han

2009

Journal of Lipid Research

Self Cite

102

109

View full text Add to dashboard Cite

show abstract

“…On the other hand, the CHO1 transcript is stabilized by respiratory deficiency (29). These forms of regulation govern the amount of PS synthase activity (16,29).…”

Section: Cho1-encoded Ps Synthase Exists In Two Forms That Differ In mentioning

confidence: 99%

“…The CHO1 transcript is primarily degraded through the general 5Ј-3Ј mRNA decay pathway that involves deadenylation, mRNA decapping, and 5Ј-3Ј-exonuclease activities (29). In wild type cells, the CHO1 transcript is moderately stable with a half-life of 12 min (29). However, defects in mitochondrial respiration stabilize the CHO1 transcript to a half-life of Ͼ45 min (29).…”

mentioning

confidence: 99%

“…However, defects in mitochondrial respiration stabilize the CHO1 transcript to a half-life of Ͼ45 min (29). This regulation results in increases in the abundance of CHO1 mRNA and PS synthase protein, enzymatic activity, and the synthesis of PS in vivo (29).…”

mentioning

confidence: 99%

“…For example, the level of the CHO1 transcript is controlled by its rate of decay (28,29). The CHO1 transcript is primarily degraded through the general 5Ј-3Ј mRNA decay pathway that involves deadenylation, mRNA decapping, and 5Ј-3Ј-exonuclease activities (29).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Phosphorylation of Yeast Phosphatidylserine Synthase by Protein Kinase A

Hyun-Min

Han

Carman

2010

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

The CHO1-encoded phosphatidylserine synthase from Saccharomyces cerevisiae is phosphorylated and inhibited by protein kinase A in vitro. CHO1 alleles bearing Ser 46 3 Ala and/or Ser 47 3 Ala mutations were constructed and expressed in a cho1⌬ mutant lacking phosphatidylserine synthase. In vitro, the S46A/S47A mutation reduced the total amount of phosphorylation by 90% and abolished the inhibitory effect protein kinase A had on phosphatidylserine synthase activity. The enzyme phosphorylation by protein kinase A, which was time-and dose-dependent and dependent on the concentration of ATP, caused a electrophoretic mobility shift from a 27-kDa form to a 30-kDa form. The two electrophoretic forms of phosphatidylserine synthase were present in exponential phase cells, whereas only the 27-kDa form was present in stationary phase cells. In vivo labeling with 32 P i and immune complex analysis showed that the 30-kDa form was predominantly phosphorylated when compared with the 27-kDa form. However, the S46A/S47A mutations abolished the protein kinase A-mediated electrophoretic mobility shift. The S46A/S47A mutations also caused a 55% reduction in the total amount of phosphatidylserine synthase in exponential phase cells and a 66% reduction in the amount of enzyme in stationary phase cells. In phospholipid composition analysis, cells expressing the S46A/S47A mutant enzyme exhibited a 57% decrease in phosphatidylserine and a 40% increase in phosphatidylinositol. These results indicate that phosphatidylserine synthase is phosphorylated on Ser 46 and Ser 47 by protein kinase A, which results in a higher amount of enzyme for the net effect of stimulating the synthesis of phosphatidylserine. In the yeast Saccharomyces cerevisiae, the CHO1-encoded3 is an ER-associated enzyme that catalyzes the formation of PS by a Mn 2ϩ -dependent sequential reaction by displacing CMP from CDP-DAG with serine ( Fig. 1) (1-5). The enzyme possesses a CDP-alcohol phosphotransferase motif, DGX 2 ARX 7,8 GX 3 DX 3 D (residues 130 -152) within a larger domain that is common to phospholipid biosynthetic enzymes (e.g. PI synthase) that catalyze similar types of reactions (6) (Fig. 1). The reaction product PS is a major membrane phospholipid in yeast, accounting for up to 18% of the total membrane phospholipids (7-9). In addition, PS synthase catalyzes the committed step in the synthesis of PE and PC via the de novo CDP-DAG pathway (7-11) (Fig. 1). As such, PS synthase is one of the most highly regulated enzymes of phospholipid synthesis in this organism (10,12,13).Genetic and biochemical mechanisms regulate the expression and activity of PS synthase. The expression of CHO1 is regulated by water-soluble phospholipid precursors (e.g. inositol, choline, ethanolamine, and serine) (14 -18), essential nutrients (e.g. zinc) (19), and growth phase (20, 21). These forms of regulation occur through a regulatory circuit involving a UAS INO cis-acting element in the CHO1 promoter, the positive transcription factors Ino2p and Ino4p, and the transcriptional repressor...

show abstract

Current awareness on yeast

2008

Yeast

View full text Add to dashboard Cite

In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Reviews; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 5th. Mar. 2008)

show abstract

Respiratory Deficiency Mediates the Regulation of CHO1-encoded Phosphatidylserine Synthase by mRNA Stability in Saccharomyces cerevisiae

Cited by 9 publications

References 97 publications

Regulation of phospholipid synthesis in yeast

Regulation of phospholipid synthesis in yeast

Phosphorylation of Yeast Phosphatidylserine Synthase by Protein Kinase A

Current awareness on yeast

Contact Info

Product

Resources

About