Respiratory Epithelial Cells Respond to Lactobacillus plantarum but Provide No Cross-Protection against Virus-Induced Inflammation

Mai, Eric; Percopo, Caroline M.; Limkar, Ajinkya R; Sek, Albert C.; Ma, Michelle; Rosenberg, Helene F.

doi:10.3390/v13010002

Cited by 6 publications

(6 citation statements)

References 85 publications

(152 reference statements)

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“…These contrasting results were also reported by Rosenberg and colleagues. The researchers demonstrated that the nasal priming of mice with the HK immunobiotic L. plantarum NCIMB 8826 provided protection against the lethal sequelae of PVM [ 14 , 15 , 16 ] and IFV [ 48 ] infections. The in vivo protective effects of the NCIMB 8826 strain were not related to the reduction in virus replication and clearance, but to efficient regulation of the inflammatory response that minimized the lung damage [ 15 , 16 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…The researchers demonstrated that the nasal priming of mice with the HK immunobiotic L. plantarum NCIMB 8826 provided protection against the lethal sequelae of PVM [ 14 , 15 , 16 ] and IFV [ 48 ] infections. The in vivo protective effects of the NCIMB 8826 strain were not related to the reduction in virus replication and clearance, but to efficient regulation of the inflammatory response that minimized the lung damage [ 15 , 16 , 48 ]. Of note, studies in two cell culture models, MLE-12 cells (mouse lung type II alveolar epithelial like-cells) and mTECs cells (polarized mouse tracheal epithelial cells) revealed the inability of HK L. plantarum NCIMB 8826 to reduce the production of CCL2, CCL5, CXCL1, and CXCL10 induced by IFV infection, using a protocol that replicated experimental conditions that were effective in vivo [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…The in vivo protective effects of the NCIMB 8826 strain were not related to the reduction in virus replication and clearance, but to efficient regulation of the inflammatory response that minimized the lung damage [ 15 , 16 , 48 ]. Of note, studies in two cell culture models, MLE-12 cells (mouse lung type II alveolar epithelial like-cells) and mTECs cells (polarized mouse tracheal epithelial cells) revealed the inability of HK L. plantarum NCIMB 8826 to reduce the production of CCL2, CCL5, CXCL1, and CXCL10 induced by IFV infection, using a protocol that replicated experimental conditions that were effective in vivo [ 48 ]. These, and our results presented here indicate that the ability of nasally administered HK L. plantarum to induce modifications in the respiratory antiviral immune response in vivo would depend on the interaction of lactobacilli with immune cells and not with epithelial cells of the respiratory tract.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Immunobiotic Lactobacilli Improve Resistance of Respiratory Epithelial Cells to SARS-CoV-2 Infection

et al. 2021

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Previously, we reported that immunomodulatory lactobacilli, nasally administered, beneficially regulated the lung antiviral innate immune response induced by Toll-like receptor 3 (TLR3) activation and improved protection against the respiratory pathogens, influenza virus and respiratory syncytial virus in mice. Here, we assessed the immunomodulatory effects of viable and non-viable Lactiplantibacillus plantarum strains in human respiratory epithelial cells (Calu-3 cells) and the capacity of these immunobiotic lactobacilli to reduce their susceptibility to the acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Immunobiotic L. plantarum MPL16 and CRL1506 differentially modulated IFN-β, IL-6, CXCL8, CCL5 and CXCL10 production and IFNAR2, DDX58, Mx1 and OAS1 expression in Calu-3 cells stimulated with the TLR3 agonist poly(I:C). Furthermore, the MPL16 and CRL1506 strains increased the resistance of Calu-3 cells to the challenge with SARS-CoV-2. L. plantarum MPL16 induced these beneficial effects more efficiently than the CRL1506 strain. Of note, neither non-viable MPL16 and CRL1506 strains nor the non-immunomodulatory strains L. plantarum CRL1905 and MPL18 could modify the resistance of Calu-3 cells to SARS-CoV-2 infection or the immune response to poly(I:C) challenge. To date, the potential beneficial effects of immunomodulatory probiotics on SARS-CoV-2 infection and COVID-19 outcome have been extrapolated from studies carried out in the context of other viral pathogens. To the best of our knowledge, this is the first demonstration of the ability of immunomodulatory lactobacilli to positively influence the replication of the new coronavirus. Further mechanistic studies and in vivo experiments in animal models of SARS-CoV-2 infection are necessary to identify specific strains of beneficial immunobiotic lactobacilli like L. plantarum MPL16 or CRL1506 for the prevention or treatment of the COVID-19.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Immunobiotic Lactobacilli Improve Resistance of Respiratory Epithelial Cells to SARS-CoV-2 Infection

et al. 2021

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“…They were autoclaved at 121°C for 15 min. Then, L. reuteri and B. bifidum were heat inactivated at 70°C for 30 min, washed once in sterile, endotoxin‐free phosphate‐buffered saline (PBS), resuspended in PBS with 0.1% bovine serum albumin (BSA) (Gabryszewski et al, 2011; Mai et al, 2020) and stored at −20°C. The diluent was prepared in sterile, endotoxin‐free PBS (1 × 10 7 cells ml −1 ) before using L. reuteri and B. bifidum .…”

Section: Methodsmentioning

confidence: 99%

Microbial and metabolic profiles of bronchopulmonary dysplasia and therapeutic effects of potential probiotics Limosilactobacillus reuteri and Bifidobacterium bifidum

Liu

Zhao

et al. 2022

Journal of Applied Microbiology

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Aims: Bronchopulmonary dysplasia (BPD) is a common respiratory disease in newborns; however, there is no effective treatment. We aimed to investigate the effects of the potential probiotics Limosilactobacillus reuteri and Bifidobacterium bifidum on BPD using 16S rDNA sequencing and metabolomics methods. Methods and Results: Faecal samples were collected from 10 BPD patients and 10 healthy subjects. 16S rDNA sequencing results showed that microbial diversity was decreased and compositions were affected in BPD. Escherichia-Shigella and Clostridium_sensu_stricto_1 were increased in the BPD group, and Enterobacteriaceae, Megamonas, Blautia, Lactobacillus (Limosilactobacillus), [Eubacterium]_coprostanoligenes_group, Phascolarctobacterium and Bifidobacterium were reduced. Metabolomics analysis identified 129 differentiated metabolites that were changed in BPD patients, and they were associated with a preference for carbohydrate metabolism in translation and metabolism during genetic information processing. Correlation analysis revealed a remarkable relationship between gut microbiota and metabolites. Subsequently, a BPD cell model was constructed to test the effect of the potential probiotics. Cell function experiments verified that treatment with the potential probiotics L. reuteri and B. bifidum promoted proliferation and inhibited apoptosis of hyperoxia-induced MLE-12 cells. In addition, treatment with the potential probiotics L. reuteri and B. bifidum reduced inflammation and oxidative stress damage. Conclusions: Treatment with the potential probiotics L. reuteri and B. bifidum could alleviate BPD and reduce inflammation and oxidative stress damage. Significance and Impact: This study was the first to report positive roles for the potential probiotics L. reuteri and B. bifidum in BPD. The potential probiotics L. reuteri and B. bifidum were shown to reduce inflammation and oxidative stress damage in BPD. This study provided new insights on the pathogenesis and treatment of BPD.

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“…Relative expression of proinflammatory and immunomodulatory mediators in BAL fluid of mice inoculated with a lethal vs. sublethal dose of PVM (day 7) and mice inoculated with a sublethal dose of PVM alone (day 7 vs. day 21) was evaluated using the Proteome Profiler Array, Panel A and the Mouse Angiogenesis Profiler Array (both from R&D Systems, Minneapolis, MN, USA) as previously described [33]. Each profiler membrane was probed with 500 µL BAL fluid (100 µL from each of 5 mice per group).…”

Section: Profiling and Elisasmentioning

confidence: 99%

Persistent Airway Hyperresponsiveness Following Recovery from Infection with Pneumonia Virus of Mice

Limkar

Percopo

Redes

et al. 2021

Viruses

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Respiratory virus infections can have long-term effects on lung function that persist even after the acute responses have resolved. Numerous studies have linked severe early childhood infection with respiratory syncytial virus (RSV) to the development of wheezing and asthma, although the underlying mechanisms connecting these observations remain unclear. Here, we examine airway hyperresponsiveness (AHR) that develops in wild-type mice after recovery from symptomatic but sublethal infection with the natural rodent pathogen, pneumonia virus of mice (PVM). We found that BALB/c mice respond to a limited inoculum of PVM with significant but reversible weight loss accompanied by virus replication, acute inflammation, and neutrophil recruitment to the airways. At day 21 post-inoculation, virus was no longer detected in the airways and the acute inflammatory response had largely resolved. However, and in contrast to most earlier studies using the PVM infection model, all mice survived the initial infection and all went on to develop serum anti-PVM IgG antibodies. Furthermore, using both invasive plethysmography and precision-cut lung slices, we found that these mice exhibited significant airway hyperresponsiveness at day 21 post-inoculation that persisted through day 45. Taken together, our findings extend an important and versatile respiratory virus infection model that can now be used to explore the role of virions and virion clearance as well as virus-induced inflammatory mediators and their signaling pathways in the development and persistence of post-viral AHR and lung dysfunction.

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Respiratory Epithelial Cells Respond to Lactobacillus plantarum but Provide No Cross-Protection against Virus-Induced Inflammation

Cited by 6 publications

References 85 publications

Immunobiotic Lactobacilli Improve Resistance of Respiratory Epithelial Cells to SARS-CoV-2 Infection

Immunobiotic Lactobacilli Improve Resistance of Respiratory Epithelial Cells to SARS-CoV-2 Infection

Microbial and metabolic profiles of bronchopulmonary dysplasia and therapeutic effects of potential probiotics Limosilactobacillus reuteri and Bifidobacterium bifidum

Persistent Airway Hyperresponsiveness Following Recovery from Infection with Pneumonia Virus of Mice

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