Respiratory Metabolism in Achlya Ambisexualis

Abstract

Manometric and spectrophotometric techniques were utilized in studying respiratory metabolism in intact mycelia and cell‐free extracts of the heterothallic water mold, Achlya ambisexualis Raper. Enzymes representing the hexose monophosphate pathway, the Embden‐Meyerhof‐Parnas scheme, the tricarboxylic acid (TCA) cycle and a terminal cytochrome system were demonstrated. In addition, glycerol phosphate dehydrogenase and lactic dehydrogenase activities were observed. Activities of selected enzymes were observed to decline with culture‐age, with the exceptions of glucose‐6‐phosphate dehydrogenase and glycerol‐phosphate dehydrogenase. Oxygen uptake in the male strain more than doubled in the presence of sexual hormone A.