Respiratory metabolism of salivary glands during the late larval and prepupal development of Drosophila melanogaster

Distinct metabolic profiles in Drosophila sperm and somatic tissues revealed by two-photon NAD(P)H and FAD autofluorescence lifetime imaging

“…1–4). For example, we found high OxPHOS activity in larval SGCs, an observation that is in agreement with their high oxygen consumption observed prior to pupariation 42 . We also note that the three parameters - not independent but based on different analyses - largely agreed with one another.…”

Terrestrial Insects with Tracheae Breath by Actively Regulating Ventilatory Movements: Physiological Similarities to Humans

“…The cumulative records of O2 consumption required precise offset regulation which was achieved by electronic zeroing and setting the offset of the scanning episodes. The method revealed subnanoliter rates of O2 consumptions/min, which was used for determination of metabolic rates in very small insects, such as ants, aphids or termites (Sláma and Denlinger 1992, Sláma and Jedlička 2012, Sláma et al 2007), or explanted insect organs, such as the salivary glands of Drosophila (Farkaš and Sláma 2015).…”

“…Hypermetabolism is used to produce metabolic water by means of complete combustion of the dietary lipid Lukáš 2011, 2016). The microrespirographic method was used for measurement of O2 consumption by the salivary glands in Drosophila melanogaster Meigen, used for the study of gene activation (Farkaš and Sláma 2015). Unfortunately, the role of the neuroendocrine system in regulation of insect respiration by extracardiac pulsations in haemocoelic pressure (Sláma 1988(Sláma , 2009(Sláma , 2010Sláma and Denlinger 1992;Sláma and Jedlička 2012) remains mostly unknown due to technical problems involved in the recording of haemocoelic pressure.…”

Endosomal vacuoles of the prepupal salivary glands of Drosophila play an essential role in the metabolic reallocation of iron

“…Once neutralized, the vacuoles disappear through a process of consolidation with ER membranes. This indicates the implementation of a developmentally-linked anabolic program (Farkaš & Sláma, 2015). Although Drosophila has been extensively studied as a model system to understand endosomal trafficking in membrane receptor internalization, lysosome maturation, tubular organ growth during embryogenesis, neural development, differentiation of imaginal discs, synaptic transmission, epithelial morphogenesis, dynamin-independent endocytosis and adherens junctions formation (Alone et al, 2005;Broadus & Doe, 1997;Brüser & Bogdan, 2017;Entchev, Schwabedissen, & González-Gaitán, 2000;Hemalatha, Prabhakara, & Mayor, 2016;Jacomin, Fauvarque, & Taillebourg, 2016;Li, Satoh, & Ready, 2007;Murthy, Teodoro, Miller, & Schwarz, 2010;Pelissier, Chauvin, & Lecuit, 2003;Perez-Mockus, Roca, Mazouni, & Schweisguth, 2017;Pinal & Pichaud, 2011;Uytterhoeven, Kuenen, Kasprowicz, Miskiewicz, & Verstreken, 2011;Wu et al, 1999), this heavy endosomal traffic in PP SGs was unanticipated.…”

“…Indeed, we demonstrated that the early prepupal SGs are metabolically active even after glue exocytosis: They display rapid changes in their respiratory activity with peaks about 3-4 hr APF. This indicates the implementation of a developmentally-linked anabolic program (Farkaš & Sláma, 2015). Based on these data, we have developed a working hypothesis that one key role, among the others that are possible, of the tremendous vacuolation and associated heavy endosomal traffic is to provide a fundamental metabolic need.…”