Respiratory Syncytial Virus Exacerbates OVA-mediated asthma in mice through C5a-C5aR regulating CD4+T cells Immune Responses

Hu, Xinyue; Li, Xiaozhao; Hu, Chengping; Qin, Ling; He, Ruoxi; Luo, Lisha; Tang, Wei; Feng, Juntao

doi:10.1038/s41598-017-15471-w

Cited by 23 publications

(26 citation statements)

References 35 publications

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“…These data suggest that C3aR1 regulates the generation of pathogenic neutrophil-recruiting T H 17 responses during hRSV infection [ 260 ]. On the other hand, another study shows that treatment with C5aRA—an antagonist of the C5a receptor—leads to lower pulmonary damage, lower AHR and lower infiltration of neutrophils in the lung and BAL, as well as a reduced viral load in the lung during hRSV infection [ 261 ]. Moreover, AHR and the infiltration of inflammatory cells are also reduced in asthma murine models during hRSV infection [ 261 ].…”

Section: Differential Regulation Of the Innate Immune Response By mentioning

confidence: 99%

“…On the other hand, another study shows that treatment with C5aRA—an antagonist of the C5a receptor—leads to lower pulmonary damage, lower AHR and lower infiltration of neutrophils in the lung and BAL, as well as a reduced viral load in the lung during hRSV infection [ 261 ]. Moreover, AHR and the infiltration of inflammatory cells are also reduced in asthma murine models during hRSV infection [ 261 ]. These data suggest that the activation of C5aR is also pathogenic and enhances AHR and pulmonary infiltration during hRSV infection and is possibly implicated in the control of asthmatic responses [ 261 ].…”

Section: Differential Regulation Of the Innate Immune Response By mentioning

confidence: 99%

“…Moreover, AHR and the infiltration of inflammatory cells are also reduced in asthma murine models during hRSV infection [ 261 ]. These data suggest that the activation of C5aR is also pathogenic and enhances AHR and pulmonary infiltration during hRSV infection and is possibly implicated in the control of asthmatic responses [ 261 ]. This is especially important, considering that C5a levels are increased in lung and BAL, and C5aR expression is upregulated in airway epithelial cells during hRSV infection [ 261 ].…”

Section: Differential Regulation Of the Innate Immune Response By mentioning

confidence: 99%

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Innate Immune Components That Regulate the Pathogenesis and Resolution of hRSV and hMPV Infections

Andrade

Pacheco

Gálvez

et al. 2020

Viruses

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The human respiratory syncytial virus (hRSV) and human Metapneumovirus (hMPV) are two of the leading etiological agents of acute lower respiratory tract infections, which constitute the main cause of mortality in infants. However, there are currently approved vaccines for neither hRSV nor hMPV. Moreover, despite the similarity between the pathology caused by both viruses, the immune response elicited by the host is different in each case. In this review, we discuss how dendritic cells, alveolar macrophages, neutrophils, eosinophils, natural killer cells, innate lymphoid cells, and the complement system regulate both pathogenesis and the resolution of hRSV and hMPV infections. The roles that these cells play during infections by either of these viruses will help us to better understand the illnesses they cause. We also discuss several controversial findings, relative to some of these innate immune components. To better understand the inflammation in the lungs, the role of the respiratory epithelium in the recruitment of innate immune cells is briefly discussed. Finally, we review the main prophylactic strategies and current vaccine candidates against both hRSV and hMPV.

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Section: Differential Regulation Of the Innate Immune Response By mentioning

confidence: 99%

Section: Differential Regulation Of the Innate Immune Response By mentioning

confidence: 99%

Section: Differential Regulation Of the Innate Immune Response By mentioning

confidence: 99%

See 1 more Smart Citation

Innate Immune Components That Regulate the Pathogenesis and Resolution of hRSV and hMPV Infections

Andrade

Pacheco

Gálvez

et al. 2020

Viruses

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show abstract

“…For 5 mice in each group, the right lung of each animal was dissected for homogenate culture and the left lung for H&E staining. For the other 5 mice in each group, whole lung homogenate was performed as previously described (15). Briefly, whole lung tissue from each animal was prepared by homogenization in PBS-containing protease inhibitors (Complete, Roche Diagnostics).…”

Section: Experimental Designmentioning

confidence: 99%

Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung

Jiang¹,

Li²,

Li³

2018

J. Thorac. Dis.

Self Cite

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Background: Acinetobacter baumannii (A. baumannii) is one of the most troublesome opportunistic pathogens associated with hospital-acquired pneumonia (HAP). It is important to be able to discriminate A. baumannii colonization from infection in its early stages so that effective antibiotics can be promptly applied.Recent studies have reported that the secretion of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) is markedly upregulated in pneumonia and sepsis, but the expression pattern of sTREM-1 in A. baumannii colonization and infection in the lung has not been explored.Methods: C57BL/6J male mice were intraperitoneally injected with 1% streptozotocin for 5 consecutive days to establish diabetic models. Subsequently, aerosol inhalation of A. baumannii suspension was performed in these mice to induce pulmonary colonization or infection with saline as vehicle control. Mice were sacrificed and lung tissue was harvested on days 0, 1, 3, 5 and 7 after exposure. Pharyngeal swab culture, lung homogenate culture, and H&E staining of lung tissue were performed to assess the severity of infectious inflammation. sTREM-1 expressions in serum and lung supernatants, serum procalcitonin (PCT) and C-reactive protein (CRP) concentrations were measured by ELISA. Results: A. baumannii colonization and infection models were verified by pharyngeal swab culture, lung homogenate culture, and H&E staining. While sTREM-1 concentrations in mice with A. baumannii colonization remained unchanged in serum and lung supernatants, sTREM-1 expression levels in infected animals were significantly upregulated. In addition, serum sTREM-1 concentration was positively correlated with serum levels of PCT and CRP. Conclusions: Dynamic secretion of sTREM-1 is associated with the development of A. baumannii infection in the lung. Therefore, sTREM-1 expression level may be a promising biomarker for discriminating A. baumannii infection from colonization.

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“…Similar to the symptoms observed in the trial participants, FIRSV has been shown to induce a Th2-biased immune response leading to pulmonary inflammation, airway obstruction and mucus hypersecretion in many animal models, which are now deemed as the hallmarks of vaccine-induced enhanced respiratory disease (ERD) 13 – 16 . Moreover, non-neutralizing antibodies induced by FIRSV have been implicated in ERD development 17 – 19 , while another major facet of immunity, subsets of CD4+ T cells, was implicated in mediating various parameters of FIRSV-induced ERD 20 , 21 . However, the contribution of memory CD8 T cells in providing protection against RSV re-infection remains to be fully understood in spite of their known importance in viral clearance 20 , 22 , 23 .…”

Section: Introductionmentioning

confidence: 99%

Targeting CD40 enhances antibody- and CD8-mediated protection against respiratory syncytial virus infection

Muralidharan

Russell

Larocque

et al. 2018

Sci Rep

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Respiratory Syncytial Virus (RSV) infects almost all children under the age of one and is the leading cause of hospitalization among infants. Despite several decades of research with dozens of candidate vaccines being vigorously evaluated in pre-clinical and clinical studies, there is no licensed vaccine available to date. Here, the RSV fusion protein (F) was fused with CD40 ligand and delivered by an adenoviral vector into BALB/c mice where the CD40 ligand serves two vital functions as a molecular adjuvant and an antigen-targeting molecule. In contrast to a formaldehyde-inactivated vaccine, the vectored vaccine effectively protected animals against RSV without inducing enhanced respiratory disease. This protection involved a robust induction of neutralizing antibodies and memory CD8 T cells, which were not observed in the inactivated vaccine group. Finally, the vectored vaccine was able to elicit long-lasting protection against RSV, one of the most challenging issues in RSV vaccine development. Further studies indicate that the long lasting protection elicited by the CD40 ligand targeted vaccine was mediated by increased levels of effector memory CD8 T cell 3 months post-vaccination.

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Respiratory Syncytial Virus Exacerbates OVA-mediated asthma in mice through C5a-C5aR regulating CD4+T cells Immune Responses

Cited by 23 publications

References 35 publications

Innate Immune Components That Regulate the Pathogenesis and Resolution of hRSV and hMPV Infections

Innate Immune Components That Regulate the Pathogenesis and Resolution of hRSV and hMPV Infections

Expression pattern of soluble triggering receptor expressed on myeloid cells-1 in mice with Acinetobacter baumannii colonization and infection in the lung

Targeting CD40 enhances antibody- and CD8-mediated protection against respiratory syncytial virus infection

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