Respiratory Tract Viral Infections in Bone Marrow Transplant Patients

Raboni, Sônia Mara; Nogueira, Meri Bordignon; Tsuchiya, Luine R. V.; Takahashi, Gislene A.; Pereira, Luciane Aparecida; Pasqüini, Ricardo; Siqueira, Marilda M.

doi:10.1097/01.tp.0000072012.26176.58

Cited by 121 publications

(86 citation statements)

References 14 publications

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“…The respiratory syncytial virus (RSV) is considered to be the most common viral agent of lower respiratory tract infections in infants and young children, and can cause serious respiratory illnesses 5,7,12,20 .The highest morbidity rates are observed in infants, in the elderly and in the immunocompromised 4,9,17,18,22 . Viral isolation in cell cultures continues to be considered the most definitive test for diagnosis 6 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants

Reis

Fink

Machado

et al. 2008

Rev. Inst. Med. trop. S. Paulo

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SUMMARYA total of 316 samples of nasopharyngeal aspirate from infants up to two years of age with acute respiratory-tract illnesses were processed for detection of respiratory syncytial virus (RSV) using three different techniques: viral isolation, direct immunofluorescence, and PCR. Of the samples, 36 (11.4%) were positive for RSV, considering the three techniques. PCR was the most sensitive technique, providing positive findings in 35/316 (11.1%) of the samples, followed by direct immunofluorescence (25/316, 7.9%) and viral isolation (20/315, 6.3%) (p < 0.001). A sample was positive by immunofluorescence and negative by PCR, and 11 (31.4%) were positive only by RT-PCR. We conclude that RT-PCR is more sensitive than IF and viral isolation to detect RSV in nasopharyngeal aspirate specimens in newborn and infants.

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Section: Introductionmentioning

confidence: 99%

Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants

Reis

Fink

Machado

et al. 2008

Rev. Inst. Med. trop. S. Paulo

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“…Health care workers (HCWs) are in direct contact with HAdV-infected patients, and this population is especially susceptible to infection with the virus, acting as a source of infection during outbreaks. Furthermore, there have been few studies in this population, and little is known about HAdV circulation in HCWs compared to community subjects 3 .…”

Section: Human Adenovirus Detection Among Immunocompetent and Immunocmentioning

confidence: 99%

“…In Brazil, there is a lack of sufficient information about the immunocompromised population, with detection ranging from 0-3% 3,9 .…”

Section: Human Adenovirus Detection Among Immunocompetent and Immunocmentioning

confidence: 99%

Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection

Watanabe

Carraro²,

Camargo

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction: Human adenoviruses (HAdV) play an important role in the aetiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was to detect HAdV using two different methods, direct fluorescence assay (DFA) and nested polymerase chain reaction (nested PCR), in samples collected from patients with acute respiratory infection (ARI) within 7 days of symptom onset. Methods: Samples (n=643) were collected from patients in different risk groups from 2001 to 2010: 139 adult emergency room patients (ERP); 205 health care workers (HCW); 69 renal transplant outpatients (RTO); and 230 patients in a haematopoietic stem cell transplantation program (HSCT). Results: Adenovirus was detected in 13.2% of the 643 patients tested by DFA and/or PCR: 6/139 (4.3%) adults in the ERP group, 7/205 (3.4%) in the HCW group, 4/69 (5.8%) in the RTO group and 68/230 (29.5%) in the HSCT patient group. Nested PCR had a higher detection rate (10%) compared with the DFA test (3.8%) (p<0.001). HSCT patients exhibited a significantly higher rate of HAdV infection. Conclusions: The adenovirus detection rate of the nested PCR assay was higher than that of the DFA test. However, the use of molecular methods in routine diagnostic laboratory work should be evaluated based on the specific circumstances of individual health services.

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“…26 In particular, influenza accounts for 20% of respiratory virus infections in the patients who receive a transplant. 27 The ability to respond to influenza vaccination is impaired well beyond the quantitative repletion of lymphocytes. 13,28 Strategies to accelerate and augment the recovery and function of autologous T cells after transplantation for myeloma may be beneficial.…”

Section: Introductionmentioning

confidence: 99%

Transfer of influenza vaccine–primed costimulated autologous T cells after stem cell transplantation for multiple myeloma leads to reconstitution of influenza immunity: results of a randomized clinical trial

Stadtmauer¹,

Vogl²,

Prak

et al. 2011

Blood

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Severe immune deficiency follows autologous stem cell transplantation for multiple myeloma and is associated with significant infectious morbidity. This study was designed to evaluate the utility of a pretransplantation vaccine and infusion of a primed autologous T-cell product in stimulating specific immunity to influenza. Twenty-one patients with multiple myeloma were enrolled from 2007 to 2009. Patients were randomly assigned to receive an influenza-primed autologous T-cell product or a nonspecifically primed autologous T-cell product. The study endpoint was the development of hemagglutination inhibition titers to the strain-specific serotypes in the influenza vaccine. Enzyme-linked immunospot assays were performed to confirm the development of influenza-specific B-cell and T-cell immunity. Patients who received the influenza-primed autologous T-cell product were significantly more likely to seroconvert in response to the influenza vaccine (P = .001). Seroconversion was accompanied by a significant B-cell response. No differences were observed in the global quantitative recovery of T-cell and B-cell subsets or in global T-cell and B-cell function. The provision of a primed autologous T-cell product significantly improved subsequent influenza vaccine responses. This trial was registered at www.clinicaltrials.gov as #NCT00499577.

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Respiratory Tract Viral Infections in Bone Marrow Transplant Patients

Cited by 121 publications

References 14 publications

Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants

Comparison of direct immunofluorescence, conventional cell culture and polymerase chain reaction techniques for detecting respiratory syncytial virus in nasopharyngeal aspirates from infants

Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection

Transfer of influenza vaccine–primed costimulated autologous T cells after stem cell transplantation for multiple myeloma leads to reconstitution of influenza immunity: results of a randomized clinical trial

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