2018
DOI: 10.29244/j-siltrop.8.3.208-214
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RESPON PERTUMBUHAN TUNAS SANINTEN (Castanopsis argentea (Blume) A.DC.) TERHADAP PEMBERIAN ZAT PENGATUR TUMBUH BAP DAN IAA SECARA IN VITRO The growth response of Saninten (Castanopsis argentea (Blume) A.DC.) In Vitro by Adding Plant Growth Regulator BAP

Abstract: Saninten (Castanopsis argentea (Blume) A.DC.) is one of the member Fagaceae family which can produce wood and non-wood product. Micropropagation of saninten by in vitro has never been reported. This research aims to identify the growth response of saninten shoot by adding Plant Growth Regulator (PGR), they are 6-benzylaminopurine (BAP) and indole-3-acetic-acid (IAA) with different concentrations in propagation in vitro on woody plant medium (WPM). The research conducted a completely randomized design (CRD) fac… Show more

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Cited by 8 publications
(11 citation statements)
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“…tunas yang sama pada beberapa perlakuan yaitu 2 pereksplan. Menurut Wulandari et al (2017) menyatakan bahwa pemberian IAA dan BAP memiliki hasil mirip terhadap jumlah tunas dan persentase muncul tunas setiap perlakuan, yang diakibatkan belum tercapainya keseimbangan hormon auksin dan sitokinin yang terkandung pada media, selain itu jumlah tunas yag terbentuk juga bergantung pada kepekaan eksplan tanaman dalam menyerap hormon berbeda.…”
Section: Pembahasanunclassified
“…tunas yang sama pada beberapa perlakuan yaitu 2 pereksplan. Menurut Wulandari et al (2017) menyatakan bahwa pemberian IAA dan BAP memiliki hasil mirip terhadap jumlah tunas dan persentase muncul tunas setiap perlakuan, yang diakibatkan belum tercapainya keseimbangan hormon auksin dan sitokinin yang terkandung pada media, selain itu jumlah tunas yag terbentuk juga bergantung pada kepekaan eksplan tanaman dalam menyerap hormon berbeda.…”
Section: Pembahasanunclassified
“…The research location was located in Materials and equipment Materials and tools needed for research include container, sprayer, cutting scissors, Erlenmeyer, measuring cup, pH meter, volumetric pipette, analytical balance, oven, autoclave, tweezers, stainless scissors, petri dish, blender, culture bottle, laminar air flow, culture rack, aluminum foil, plastic wrap, handsprayer, C. odorata forma Genuina from SEAMEO Biotrop, systemic fungicide (50.4% benomyl), systemic bactericidal (20% streptomycin sulfate), 96% ethanol, sterile distilled water, detergent, alcohol, tween-20, NaOCl, WPM media (Woody Plant medium), MS medium (Murashige and Skoog), agar, granulated sugar, BAP, NAA. The cleaned explants were then aseptically grown in WPM plus broad-spectrum antibiotic media in the LAF The culture bottles were then stored on shelves in the culture room at 22-25 °C air temperature and under one unit of 36-40 watt fluorescent lamps [10]. The design used for the sterilization stage was a completely randomized design.…”
Section: Research Locationmentioning
confidence: 99%
“…Data on the results of explants sterilization were tested with ANOVA; if the analysis results were significantly different, and then continued with the Duncan's advanced test [17]. Callus scoring data were analyzed using Kruskal-Wallis analysis [10]. Data was processed using Minitab, Microsoft Excel, and SAS version 9.1.…”
Section: Research Locationmentioning
confidence: 99%
“…Akan tetapi saat ini jumlah C. argentea (Blume) A.DC. semakin menurun karena tingkat pertumbuhan yang lama, adanya pembalakan dalam pengambilan kayunya dan permudaan alami yang sedikit (Wulandari, Arum Sekar, 2017). Banyaknya manfaat tanaman ini menjadikan tanaman ini harus dilakukan perbanyakan agar tidak punah.…”
Section: Pendahuluanunclassified
“…Penelitian (Wulandari, Arum Sekar, 2017) menyatakan bahwa terjadinya kontaminasi pada eksplan yang telah dilakukan proses sterilisasi diduga dikarenakan proses sterilisasi yang kurang optimal. Menurut (Zulkarnain, 2009), kontaminasi mikroorganisme yang terjadi berasal dari berbagai faktor diantaranya alat yang digunakan pada proses sterilisasi tidak sempurna, tempat kerja yang masih kurang steril, berasal dari eksplan ataupun proses pengerjaan yang kurang teliti.…”
Section: Hasil Dan Pembahasanunclassified