Responds of Bone Cells to Microgravity: Ground-Based Research

Zhang, Jian; Li, Jingbao; Xu, Huiyun; Yang, Pengfei; Xie, Li; Qian, Airong; Zhao, Yong; Shang, Peng

doi:10.1007/s12217-015-9443-z

Cited by 9 publications

(6 citation statements)

References 76 publications

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“…As we all know, bone remodeling depends on the dynamic balance in normal bones. It has been shown that microgravity environment breaks the balance between them, which may count for the bone loss [33]. At the same time, the effects of microgravity on osteoblast alterations have been widely reported, including reduced proliferation, activity and osteoblastic function of osteoblast, as well as inhibition the response to bone related factors [34][35][36].…”

Section: Discussionmentioning

confidence: 99%

Osteogenic differentiation system based on biopolymer nanoparticles for stem cells in simulated microgravity

et al. 2021

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An efficient long-term intracellular growth factor release system in simulated microgravity for osteogenic differentiation was prepared based on polylactic acid (PLA) and polyhydroxyalkanoate (PHA) nanoparticles (NPs) for loading of bone morphogenetic protein 2 (BMP2) and bone morphogenetic protein 7 (BMP7) (defined as sB2-PLA-NPs and sB7-PHA-NPs), respectively, associated with osteogenic differentiation of human adipose derived stem cells (hADSCs). On account of soybean lecithin (SL) as biosurfactants, sB2-PLA-NPs and sB7-PHA-NPs had a high encapsulation efficiency (>80%) of BMPs and uniform small size (<100 nm), and showed a different slow-release to provide BMP2 in early stage and BMP7 in late stages of osteogenic differentiation within 20 d, due to degradation rate of PLA and PHA in cells. After uptake into hADSCs, by comparison with single sB2-PLA-NPs or sB7-PHA-NPs, the Mixture NPs compound of sB2-PLA-NP and sB7-PHA-NP with a mass ratio of 1:1, can well-promote ALP activity, expression of OPN and upregulated related osteo-genes. Directed osteo-differentiation of mixture NPs was similar to result of sustained free-BMP2 and BMP7-supplying (sFree-B2&B7) in simulated microgravity, which demonstrated the reliability and stability of Mixture NPs as a long-term osteogenic differentiation system in space medicine and biology in future.

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Section: Discussionmentioning

confidence: 99%

Osteogenic differentiation system based on biopolymer nanoparticles for stem cells in simulated microgravity

et al. 2021

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“…Afterwards, osteoblast differentiation and mineralization were inhibited in HyMF of 500 nT through restraining the activity of ALP, the deposition of calcium and the formation of mineralized nodule. During long-term space exploration, osteoporosis tends to occur especially in load-bearing bone for astronauts due to lacked gravity stress [ 42 , 43 ]. Data from Mir and ISS space stations shows that mechanical stimulation in the form of exercise is still not enough to prevent bone loss in long-duration spaceflight [ 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Regulation of Osteoblast Differentiation and Iron Content in MC3T3-E1 Cells by Static Magnetic Field with Different Intensities

Yang

Zhang

Ding

et al. 2017

Biol Trace Elem Res

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Many studies have indicated that static magnetic fields (SMFs) have positive effects on bone tissue, including bone formation and bone healing process. Evaluating the effects of SMFs on bone cell (especially osteoblast) function and exploring the mechanism, which is critical for understanding the possible risks or benefits from SMFs to the balance of bone remodeling. Iron and magnetic fields have the natural relationship, and iron is an essential element for normal bone metabolism. Iron overload or deficiency can cause severe bone disorders including osteoporosis. However, there are few reports regarding the role of iron in the regulation of bone formation under SMFs. In this study, hypomagnetic field (HyMF) of 500 nT, moderate SMF (MMF) of 0.2 T, and high SMF (HiMF) of 16 T were used to investigate how osteoblast (MC3T3-E1) responses to SMFs and iron metabolism of osteoblast under SMFs. The results showed that SMFs did not pose severe toxic effects on osteoblast growth. During cell proliferation, iron content of osteoblast MC3T3-E1 cells was decreased in HyMF, but was increased in MMF and HiMF after exposure for 48 h. Compared to untreated control (i.e., geomagnetic field, GMF), HyMF and MMF exerted deleterious effects on osteoblast differentiation by simultaneously retarding alkaline phosphatase (ALP) activity, mineralization and calcium deposition. However, when exposed to HiMF of 16 T, the differentiation potential showed the opposite tendency with enhanced mineralization. Iron level was increased in HyMF, constant in MMF and decreased in HiMF during cell differentiation. In addition, the mRNA expression of transferrin receptor 1 (TFR1) was promoted by HyMF but was inhibited by HiMF. At the same time, HiMF of 16 T and MMF of 0.2 T increased the expression of ferroportin 1 (FPN1). In conclusion, these results indicated that osteoblast differentiation can be regulated by altering the strength of the SMF, and iron is possibly involved in this process.

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“…The cells were then placed in a random positioning machine (Center for Space Science and Applied Research, Chinese Academy of Sciences), as described. 22 The rotation direction and speed (varied from 0 to 10 rpm) were random. During cell culture, the random positioning machine was placed in an incubator at 37 °C with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

“…C2C12 cells (1 × 10 6 ) were seeded on carrier slides placed in six-well plates overnight. The cells were then placed in a random positioning machine (Center for Space Science and Applied Research, Chinese Academy of Sciences), as described . The rotation direction and speed (varied from 0 to 10 rpm) were random.…”

Section: Methodsmentioning

confidence: 99%

miR-194-Loaded Gelatin Nanospheres Target MEF2C to Suppress Muscle Atrophy in a Mechanical Unloading Model

et al. 2021

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Muscle atrophy usually occurs under mechanical unloading, which increases the risk of injury to reduce the functionality of the moving system, while there is still no effective therapy until now. It was found that miR-194 was significantly downregulated in a muscle atrophy model, and its target protein was the myocyte enhancer factor 2C (MEF2C). miR-194 could promote muscle differentiation and also inhibit ubiquitin ligases, thus miR-194 could be used as a nucleic acid drug to treat muscle atrophy, whereas miRNA was unstable in vivo, limiting its application as a therapeutic drug. A gelatin nanosphere (GN) delivery system was applied for the first time to load exogenous miRNA here. Exogenous miR-194 was loaded in GNs and injected into the muscle atrophy model. It demonstrated that the muscle fiber cross-sectional area, in situ muscle contractile properties, and myogenic markers were increased significantly after treatment. It proposed miR-194 loaded in GNs as an effective treatment for muscle atrophy by promoting muscle differentiation and inhibiting ubiquitin ligase activity. Moreover, the developed miRNA delivery system, taking advantage of its tunable composition, degradation rate, and capacity to load various drug molecules with high dosage, is considered a promising platform to achieve precise treatment of muscle atrophy-related diseases.

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Responds of Bone Cells to Microgravity: Ground-Based Research

Cited by 9 publications

References 76 publications

Osteogenic differentiation system based on biopolymer nanoparticles for stem cells in simulated microgravity

Osteogenic differentiation system based on biopolymer nanoparticles for stem cells in simulated microgravity

Regulation of Osteoblast Differentiation and Iron Content in MC3T3-E1 Cells by Static Magnetic Field with Different Intensities

miR-194-Loaded Gelatin Nanospheres Target MEF2C to Suppress Muscle Atrophy in a Mechanical Unloading Model

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