Response and Adaptation Mechanisms of Tea Plant to Low-Temperature Stress

Hao, Xinyuan; Wang, Lu; Zeng, Jianming; Yang, Yajun; Wang, Xinchao

doi:10.1007/978-981-13-2140-5_3

Cited by 14 publications

(25 citation statements)

References 101 publications

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“…There are three phases of plant response to low temperature stress ( Hao et al, 2018 ): the first is acclimation, which occurs at low positive temperatures. The second is hardening, during which the maximum possible degree of frost tolerance is achieved by the plant, and the third phase is the recovery of the plant after stress.…”

Section: Discussionmentioning

confidence: 99%

“…In the current study, we used the frost tolerant Caucasian tea genotype Gruzinskii7 to try to identify the molecular mechanisms underlying strong frost tolerance. Specifically, we studied the roles played by early recognition of cold stress ( Ban et al, 2017 ; Hao et al, 2018 ; Yue et al, 2015 ), and what specific metabolic pathways, morphological traits ( Hirayama & Shinozaki, 2010 ), and putative genes could be involved in frost tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…Plants were grown in 2 liter polyethylene pots filled with brown forest acidic soil (pH = 5.0). According to the literature ( Hao et al, 2018 ) cold acclimation of tea plants started when temperature decreased lower than +10. On the other hand, winter comes not immediately after optimum growing period (+18–25 °C) in natural conditions.…”

Section: Methodsmentioning

confidence: 99%

“…Some physiological, biochemical and genetic markers of cold tolerance were proposed in certain tea genotypes ( Hao et al, 2018 ). Nevertheless, many mechanisms are still unclear because cold hardiness is a result of a combination of mechanisms involving significant structural, biochemical, and genetic adjustments ( Wisniewski, Nassuth & Arora, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Physiological, biochemical and genetic responses of Caucasian tea (Camellia sinensis(L.) Kuntze) genotypes under cold and frost stress

Самарина

Malyukova

Efremov

et al. 2020

PeerJ

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Background Cold and frost are two serious factors limiting the yield of many crops worldwide, including the tea plant (Camellia sinensis (L.) Kuntze). The acclimatization of tea plant from tropical to temperate climate regions resulted in unique germplasm in the North–Western Caucasus with extremely frost-tolerant genotypes. Methods The aim of the current research was to evaluate the physiological, biochemical and genetic responses of tolerant and sensitive tea cultivars exposed to cold (0 to +2 °C for 7 days) and frost (−6 to −8 °C for 5 days). Relative water content, cell membranes integrity, pH of the cell sap, water soluble protein, cations, sugars, amino acids were measured under cold and frost. Comparative expression of the following genes ICE1, CBF1, WRKY2, DHN1, DHN2, DHN3, NAC17, NAC26, NAC30, SnRK1.1, SnRK1.2, SnRK1.3, bHLH7, bHLH43, P5CS, LOX1, LOX6, LOX7 were analyzed. Results We found elevated protein (by 3–4 times) and cations (potassium, calcium and magnesium) contents in the leaves of both cultivars under cold and frost treatments. Meanwhile, Leu, Met, Val, Thr, Ser were increased under cold and frost, however tolerant cv. Gruzinskii7 showed earlier accumulation of these amino acids. Out of 18 studied genes, 11 were expressed at greater level in the frost- tolerant cultivar comparing with frost-sensitive one: ICE1, CBF1, WRKY2, DHN2, NAC17, NAC26, SnRK1.1, SnRK1.3, bHLH43, P5CS and LOX6. Positive correlations between certain amino acids namely, Met, Thr, Leu and Ser and studied genes were found. Taken together, the revealed cold responses in Caucasian tea cultivars help better understanding of tea tolerance to low temperature stress and role of revealed metabolites need to be further evaluated in different tea genotypes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Physiological, biochemical and genetic responses of Caucasian tea (Camellia sinensis(L.) Kuntze) genotypes under cold and frost stress

Самарина

Malyukova

Efremov

et al. 2020

PeerJ

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“…Различные по устойчивости к этому фактору культуры характеризуются разнообразными механизмами устой чивости, а у древесных культур, таких как чай (Camel lia sinensis (L.) Kuntze), ответ на стресс более сложный и комплексный (Hao et al, 2018). Для чайного растения, основная зона произрастания которого -тропические и субтропические регионы, актуальным является создание морозоустойчивых сортов, позволяющих значительно рас ширить его ареал и увеличить площади возделывания.…”

Section: Introductionunclassified

Comparative analysis of gene expression in tea plant (Camellia sinensis (L.) Kuntze) under low-temperature stress

et al. 2020

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Low-temperature stress is one of the main factors limiting the distribution and reducing the yield of many subtropical crops, including the tea crop. Efficient breeding to develop frost-tolerant cultivars requires a reliable set of genetic markers for identifying resistance donors, and that is why it is necessary to reveal the specific genetic response in frost-tolerant genotypes in comparison with frost- susceptible ones. In this work, we performed a comparative analysis of the expression of 18 tea genes (ICE1, CBF1, DHN1, DHN2, DHN3, NAC17, NAC26, NAC30, bHLH7, bHLH43, P5CS, WRKY2, LOX1, LOX6, LOX7, SnRK1.1, SnRK1.2, SnRK1.3) under cold and frost conditions in two tea genotypes, tolerant and susceptible. Low-temperature stress was induced by placing the potted plants in cold chambers and lowering the temperature to 0…+2 °С for 7 days (cold stress), followed by a decrease in temperature to –4…–6 °С for 5 days (frost stress). Relative electrical conductivity of leaf was measured in response to the stress treatments, and a significant difference in the frost tolerance of the two tea genotypes was confirmed. Cold exposure did not lead to a change in the electrical conductivity of leaf tissue. On the other hand, frost treatment resulted in increased REC in both genotypes and to a greater extent in the susceptible genotype. Increased expression of all the genes was shown during cold and frost. The genes that were strongly expressed in the tolerant tea genotype were revealed: ICE1, CBF1, DHN2, NAC17, NAC26, bHLH43, WRKY2, P5CS, LOX6, SnRK1.1, SnRK1.3. These genes can be proposed as markers for the selection of frost-tolerance donors in tea germplasm collections. Additionally, it was shown that the tolerant genotype is characterized by an earlier response to stress at the stage of cold acclimation. The study of the expression of the identified genes in different organs of tea plants and in different exposures to low temperature is relevant for further investigations.

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