Response Formation of Cattleya Orchid Leaf Callus (<i>Cattleya</i> Sp.) with the Addition of <i>2.4 Dichlorophenoxy Acetic Acid </i>and <i>6-Benzyl Amino Purine In Vitro</i>

Abstract

Orchid Cattleya sp. has a very high commercial value in the market both locally and internationally. Although the price of the orchid tends to be expensive, the value it has is much more expensive. So cattleya is always a top priority for hobbyists and collectors [1]. Cattleya is one of the orchid genera that have flowers with large sizes and is known as "The Queen of Orchids" because it has the beauty of color variations in its flowers [2]. In addition to having beauty in its flowers, bright flower color, and fragrant smell, Cattleya Orchid in general has a large flower size compared to other orchid flowers [3]. This study aimed to determine the best combination for callus growth from the orchid leaf explant Cattleya sp. with the combination of media using the ingredients MS + 6-Benzyl Amino Purine (0; 0.3; 0.6 ppm) and 2.4 Dechlorophenoxy Acetic Acid (0; 1; 2; 3 ppm) with three repetitions for each treatment. The data from the study showed that the best concentration to spur the growth of the callus of the explanatory orchid leaves of Cattleya sp. At the treatment of 2.4-D 1 ppm + BAP 0.6 ppm with a percentage of callus formed about 60% with a green callus color and compact callus texture and medium callus growth (++).

In vitro Growth of Cattleya sp Orchid from Leaf Explants with Growth Regulators

“…In this study, it was found that even though 2.4-D was added at a dose of 0; 1; 2; 3 ppm combined with kinetine at a dose of 0; 0.3; 0.6, it did not cause callus. This is contrary to what Hariyadi et al, (2023) obtained by using a combination of growth regulatory substances 2.4-D at doses (0, 1, 2, and 3 ppm) combined with BAP at doses (0, 0.3, and 0.6) to produce research results as follows: Combination treatment of 2.4-D with BAP was able to produce a response in the form of discoloration, explant swelling, and callus formation in several treatments, namely P3 (2.4-D 1 ppm + BAP 0 ppm), P5 (2.4-D 1 ppm + BAP 0.6 ppm), P7 (2.4-D 2 ppm + BAP 0.3 ppm), and P8 (2.4-D 2 ppm + BAP 0.6 ppm). The best concentration to stimulate callus growth of Cattleya orchid leaf explants was treatment P5 (2.4-D 1 ppm + BAP 0.6 ppm) with a percentage of 35% callus formation characterized by green callus color, compact callus texture, and moderate callus growth (+ +).…”

Regeneration of Cattleya Amazing Thailand in ½ MS Medium with The Addition of Several Concentration of PGR and Coconut Water

“…Kombinasi 2, dan Benzil Amino Purin (BAP) dapat meningkatkan regenerasi eksplan dan pembentukan kalus pada plantlet anggrek Cattleya sp. (Hariyadi et al, 2023). Pemanjangan sel dilakukan oleh 2,4-D sedangkan BAP berperan dalam pembelahan sel pada plantlet sehingga penggunaan kombinasi keduanya dapat memberikan efek maksimal pada regenerasi plantlet (Waryastuti et al, 2017).…”