SummaryThe Hanford Site Surface Barrier Development Program was organized in 1985 to test the effectiveness of various barrier designs in minimizing the effects of water infiltration; plant, animal, and human intrusion; and wind and water erosion on buried wastes, and in minimizing the emanation of noxious gases. Plants will serve to minimize drainage and erosion, but present the potential for growing roots into wastes. Animals burrow holes into the soil, and the burrow holes could allow water to preferentially drain into the waste. They also bring soil to the surface which, if wastes are incorporated, could present a risk for the dispersion of wastes into the environment. This report reviews work done to assess the role of plants and animals in isolation barriers at Hanford. It also reviews work done to understand the potential effects from climate change on the plants and animals that may inhabit barriers in the future.Plant studies have been done on community analysis, root characteristics, water balance, and water and wind erosion. Community analyses are reviewed in the context of native plants, invasive aliens, community dynamics, and revegetation efforts. Roots are examined with respect to water balance, other root functions, and intrusion into wastes. Water balance studies have been done at various locations on site, including McGee Ranch, the 300 North grass site, the Artemisia tridentuta/ Pseudoroegneria spicata community, and at Lower Snively field. Numerous lysimeter studies have examined the role of vegetation in water balance. Work has been reviewed to quanti@ the relationship plants have on water and wind erosion.Animal studies have focused on burrowing effects on soil water balance and the transport of buried wastes to the surface. This has been done for small and large mammals on site. Studies done to determine the role of animals on waste transport to the surface are reviewed.Studies on the role of plants and animals on barriers and climate change at Hanford were reviewed. Climate change studies have focused on assessing the potential range of climate conditions barriers may be exposed to over thousands of years and the potential consequences of such exposure on the plants and animals that may inhabit a barrier, and the water balance of a barrier. Climate change studies indicate that the present interglacial may last another 10,000 years, with the continental glacial ice reaching its maximum extent (important for the Pasco Basin because of its potential for contributing to catastrophic flooding) 100,000 years or more into the future.The optimal plant community for barrier function is one composed of deep-rooted perennial shrubs and grasses. Deep-rooted perennial shrubs and grasses will minimize the risk of water drainage into the wastes and minimize erosion. The establishment of such a community in the face of the encroachment of invasive alien species is critical for proper barrier function. Investigations into the characteristics of new invasive alien species are needed. Continued research on ...