Response of amino acid metabolism to decreased temperatures in anammox consortia: Strong, efficient and flexible

Zhou, Jianhang; Huo, Tangran; Sun, Jingqi; Feng, Yiming; Pan, Juejun; Zhao, Yunpeng; Liu, Sitong

doi:10.1016/j.biortech.2022.127099

Cited by 25 publications

(11 citation statements)

References 46 publications

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“…Amino acids are essential elements for bacterial growth, reproduction, and maintenance of the organism, and amino acid metabolism tends to be located more centrally in the metabolic network than other pathways . Among the 19 essential amino acids detected in this study, the abundance of 17 amino acids decreased, and only the abundance of 2 amino acids, cysteine and phenylalanine, did not change significantly, indicating that these were still active and may play a key role in the metabolism of the black anammox consortia.…”

Section: Discussionmentioning

confidence: 66%

“…These 8 metabolites belong to purine, vitamin B6, phenylalanine, and fatty acid metabolism. Purine, Vitamin B6, phenylalanine, and fatty acid metabolism in black anammox consortia play essential roles in bacterial stress response, supporting the adaptation and survival of anammox bacteria under starvation. − Phenylalanine metabolism is positively correlated with biofilm formation under low-temperature conditions . Upregulated fatty acids also contribute to the structural stability of anammox consortia. , Purine metabolism is associated with DNA and RNA synthesis and energy metabolism in bacteria. , Vitamin B6 plays a crucial role in protecting cells from oxidative stress .…”

Section: Discussionmentioning

confidence: 99%

“…The significantly upregulated metabolites in S1 were mainly involved in purine, Vitamin B6, phenylalanine, and fatty acid metabolism. Specifically, 19 out of 20 essential amino acids required for bacterial metabolism were detected and arranged in descending order of metabolic synthesis cost, 44,45 as shown in Figure 4(C). Among the detected amino acids, the abundance of 17 amino acids was significantly reduced in sample S1 (black consortia) compared to that in sample S2 (red consortia), and the metabolic activity of amino acids was significantly decreased under starvation conditions.…”

Section: Metabolic Profile Of Black and Red Anammox Consortiamentioning

confidence: 99%

“…62−67 Phenylalanine metabolism is positively correlated with biofilm formation under low-temperature conditions. 62 Upregulated fatty acids also contribute to the structural stability of anammox consortia. 63,64 Purine metabolism is associated with DNA and RNA synthesis and energy metabolism in bacteria.…”

Section: Blackening Mechanism Of Anammox Consortia Due To Starvationmentioning

confidence: 99%

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Chameleon-like Anammox Bacteria for Surface Color Change after Suffering Starvation

Sun,

Feng,

Zheng

et al. 2023

Environ. Sci. Technol.

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Bacteria are often exposed to long-term starvation during transportation and storage, during which a series of enzymes and metabolic pathways are activated to ensure survival. However, why the surface color of the bacteria changes during starvation is still not well-known. In this study, we found black anammox consortia suffering from long-term starvation contained 0.86 mmol gVSS −1 cytochrome c, which had no significant discrepancy compared with the red anammox consortia (P > 0.05), indicating cytochrome c was not the key issue for chromaticity change. Conversely, we found that under starvation conditions cysteine degradation is an important metabolic pathway for the blackening of the anammox consortia for H 2 S production. In particular, anammox bacteria contain large amounts of iron-rich nanoparticles, cytochrome c, and other iron−sulfur clusters that are converted to produce free iron. H 2 S combines with free iron in bacteria to form Fe−S compounds, which eventually exist stably as FeS 2 , mainly in the extracellular space. Interestingly, FeS 2 could be oxidized by air aeration, which makes the consortia turn red again. The unique self-protection mechanism makes the whole consortia appear black, avoiding inhibition by high concentrations of H 2 S and achieving Fe storage. This study expands the understanding of the metabolites of anammox bacteria as well as the bacterial survival mechanism during starvation.

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Metabolic Profile Of Black and Red Anammox Consortiamentioning

confidence: 99%

Section: Blackening Mechanism Of Anammox Consortia Due To Starvationmentioning

confidence: 99%

See 2 more Smart Citations

Chameleon-like Anammox Bacteria for Surface Color Change after Suffering Starvation

Sun,

Feng,

Zheng

et al. 2023

Environ. Sci. Technol.

Self Cite

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“…Beyond that, amino acids can be decomposed into ATP by deamination and decarboxylation to provide energy source for metabolic functions (Schinn et al,, 2021). Amino acids can also provide a carbon skeleton for amino acid synthesis through amino group transformation, from one amino acid to another amino acid, such as glutamic acid converted to aspartic acid under the action of aspartate aminotransferase (Zhou et al, 2022). Different microorganisms have different abilities of deamination, decarboxylation and amino group transformation, which is closely related to the genetic information and culture conditions of microorganisms.…”

Section: Changes In the Amino Acid Content In Cordyceps Militaris Str...mentioning

confidence: 99%

Analysis of appearance and active substances of Cordyceps militaris stromata on Antheraea pernyi pupae after optimization

LIU

XUE

et al. 2023

Food Sci. Technol

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Cordyceps militaris stromata on Antheraea pernyi pupae contain various active components, including cordycepin, adenosine, polysaccharides, and amino acids. Response surface methodology (RSM) was used to optimize the liquid culture conditions for C. militaris before its injection into A. pernyi pupae. A pH of 7.56 ± 0.02, a culture temperature of 20.5 ± 0.1 °C, a culture time of 110.5 ± 0.5 h, and a KH 2 PO 4 concentration of 1.11 ± 0.01 g l −1 resulted in a C. militaris dry weight of 1.0226 g l −1 . Experimental and predicted values were similar. The RSM optimization increased the number of fruiting bodies (17 to 22) and the average fruiting body length (6.9 cm to 7.9 cm), while also deepening the yellow colouration of the fruiting bodies. The adenosine, cordycepin, polysaccharide, carotenoid, and cordycepic acid contents increased by 12.52%, 7.67%, 3.03%, 14.93%, and 0.02%, respectively, after the optimization. However, the optimization did not alter the number of different amino acids (18) or the total amino acid content, even though the contents of certain amino acids changed somewhat. These finding may be useful for increasing the yield of C. militaris stromata on A. pernyi pupae, which will increase the profitability of C. militaris production.

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Analyzing the nitrogen removal performance and cold adaptation mechanism of immobilized cold‐acclimation ANAMMOX granules at low temperatures

Zhang,

2024

Water Environment Research

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To improve the treatment performance of anaerobic ammonium oxidation (ANAMMOX) processes at low temperatures, the immobilized cold‐acclimation ANAMMOX granules (R3) were prepared and their low‐temperature nitrogen removal ability as well as the cold adaptation mechanism were analyzed. The results indicated that the total inorganic nitrogen (TIN) removal efficiency of R3 was significantly higher than that of R2 (cold‐acclimation granules without immobilization) and R1 (common granules), especially at 11 ± 2 and 7 ± 2°C (68% and 54%). These were attributed to the remarkable biomass retention capacity of R3, high up to 4.3–4.9 mg/gVSS even at 5–18°C. Besides, higher protein (PN) content of tightly bound extracellular polymeric substances (TB‐EPS) also facilitated microbial aggregation in R3. Meanwhile, R3 granules retained higher ANAMMOX activity and heme c content at 5–25°C. The original dominant ANAMMOX genus (Candidatus Kuenenia) in R3 kept higher abundance (49%–57%) at 23 ± 2 and 16 ± 2°C, whereas Candidatus Brocadia became the dominant ANAMMOX genus (25%–32%) in R3 at 11 ± 2 and 7 ± 2°C. Notably, different ANAMMOX genera in R3 may adapt to cold environment by regulating the expression of cold‐stress proteins (CspA, CspB, PpiD, and UspA).Practitioner Points Immobilized cold‐acclimation ANAMMOX granules showed higher nitrogen removal efficiency at 23°C → 5°C. Immobilization method effectively retained biomass (Candidatus Kuenenia and Candidatus Brocadia). Immobilization facilitated TB‐EPS release and biological aggregation in cold‐acclimation granules. Expression of cold‐stress proteins in immobilized cold‐acclimation granules was more active.

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Response of amino acid metabolism to decreased temperatures in anammox consortia: Strong, efficient and flexible

Cited by 25 publications

References 46 publications

Chameleon-like Anammox Bacteria for Surface Color Change after Suffering Starvation

Chameleon-like Anammox Bacteria for Surface Color Change after Suffering Starvation

Analysis of appearance and active substances of Cordyceps militaris stromata on Antheraea pernyi pupae after optimization

Analyzing the nitrogen removal performance and cold adaptation mechanism of immobilized cold‐acclimation ANAMMOX granules at low temperatures

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