Response of avian nuclei to mammalian maturation promoting factor (MPF)

Trefil, Pavel; Horská, M; Kaňka, Jiří; Jirmanová, J; Moor, R. M.; Fulka, J.

doi:10.1017/s0967199400002719

Cited by 3 publications

(2 citation statements)

References 23 publications

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“…These results were further verified when avian Sphase nuclei were introduced into chemically enucleated mouse oocytes without MPF. Here, no DNA synthesis could be detected in transplanted nuclei (47). It appears that DNA synthesis suppressor activity is released from the germinal vesicle (GV), or activated shortly after GV breakdown, because S-phase nuclei introduced into GV-staged oocytes continued DNA synthesis(48).…”

Section: Early Experiments Showing the Beneficial Effect Of Mpf Upon mentioning

confidence: 98%

Nuclear transplantation in mammals: Remodelling of transplanted nuclei under the influence of maturation promoting factor

1996

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Whilst the role of Maturation or M-phase Promoting Factor (MPF) as a universal M-phase regulator is well documented, much less attention has been paid to its role in nuclear transplantation experiments and especially to its influence upon remodelling of transplanted nuclei. There is currently wide acceptance that successful nuclear transplantation using differentiated nuclei is possible only in a cytoplasmic environment that is capable of inducing rapid nuclear de-differentiation to a pronuclear-like form. In this review our purpose is firstly, to outline the conditions under which such remodelling can be induced, and secondly, to extend the debate to include a consideration of whether complete nuclear remodelling is an absolute necessity for clonal development.

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Section: Early Experiments Showing the Beneficial Effect Of Mpf Upon mentioning

confidence: 98%

Nuclear transplantation in mammals: Remodelling of transplanted nuclei under the influence of maturation promoting factor

1996

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“…Our results published recently demonstrate the presence of DNA synthesis suppressing activity in maturing and mature mouse oocytes (Fulka, Jr et al, 1995a, b;Trefil et al, 1995). The nature of this activity is unknown at present.…”

Section: Discussionmentioning

confidence: 54%

Induction of DNA replication in germinal vesicles and in nuclei formed in maturing mouse oocytes by 6-DMAP treatment

Fulka

First²,

C³

et al. 1997

Zygote

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Immature mouse oocytes (germinal vesicle stage, GV), oocytes at different stages during maturation (prometaphase to anaphase I) and matured oocytes (metaphase II arrested) were cultured in 6-dimethylaminopurine (6-DMAP)-supplemented medium also containing bromodeoxyuridine for the assessment of DNA replication in these cells. Immature oocytes remained arrested at the GV stage and DNA replication was never detected in them. On the other hand, oocytes at the prometaphase to anaphase-telophase I stages responded to 6-DMAP treatment by forming nuclei which synthesised DNA. Mature (metaphase II) oocytes did not respond to 6-DMAP and their chromatin remained condensed. DNA synthesis could even be induced in GV-staged oocytes, but only when they were fused to freshly activated oocytes and incubated in 6-DMAP-supplemented medium.

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Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocyte maturation

Fulka

First²,

Moor³

1998

Molecular Human Reproduction

154

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The requisite endpoint of mammalian oocyte maturation, whether in vivo or in vitro, is a metaphase II oocyte which is able to be fertilized and which can eventually support normal embryonic development. Oocytes which have been matured in vivo basically fulfill these criteria. On the other hand, a completely different situation exists when these cells are isolated from the ovaries and cultured in vitro. If they are too small (growing oocytes), they do not undergo maturation, or, if more advanced, will mature only to the metaphase I stage. Even in fully grown oocytes which are able to mature to metaphase II, the developmental potential after fertilization is disappointingly low, for reasons which remain unknown. The complexity of certain factors (nuclear, cytoplasmic or arising from our current culture systems) undoubtedly influences both the ability of oocytes to mature fully, as well as their developmental potential after fertilization.

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Response of avian nuclei to mammalian maturation promoting factor (MPF)

Cited by 3 publications

References 23 publications

Nuclear transplantation in mammals: Remodelling of transplanted nuclei under the influence of maturation promoting factor

Nuclear transplantation in mammals: Remodelling of transplanted nuclei under the influence of maturation promoting factor

Induction of DNA replication in germinal vesicles and in nuclei formed in maturing mouse oocytes by 6-DMAP treatment

Nuclear and cytoplasmic determinants involved in the regulation of mammalian oocyte maturation

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