Response of Bacterial Community Structure to Different Biochar Addition Dosages in Karst Yellow Soil Planted with Ryegrass and Daylily

Abstract

Biochar has been widely used to ameliorate soil quality and increase crop productivity through enhancement of nutrient availability and microbial community. The Karst yellow soil in China is characterized by severe soil degradation owing to intensive nutrient leaching. However, the biochar addition effects on the changes of Karst yellow soil are unclear, and the adequate number of biochar dosages to explain optimum of plant growth in this soil area remains poorly understood. In this study, pot experiments were conducted to examine the effects of biochar addition (1%, 3%, 5%, 7%, and 9% by weight; 0% as a control) on bacterial abundance and community structure via high-throughput sequencing coupled with bioinformatics methods applied to Karst yellow soil with planting ryegrass (Lolium perenne L.) and daylily (Hemerocallis fulva). After adding biochar for 188 days, significantly increased pH, soil organic matter, total nutrient contents, and bacterial abundance, but decreased available nitrogen, were observed. Changed bacterial community structures were found in biochar treatments compared with those without biochar. In both soils of planted ryegrass and daylily, the optimum soil bacterial abundance was found in 7% biochar dosage, but the lowest values were in the controls (0%). Taxonomic analysis identified that Micrococcaceae (24.53%), Oxalobacteraceae (11.87%), and Nocardioidaceae (7.89%) were the dominant family in the soil of ryegrass growth, and Micrococcaceae (16.20%), Xanthomonadaceae (6.94%), and Nocardioidaceae (6.41%) were the dominant family in soil of daylily growth. Canonical correspondence analysis showed that the alterations of soil bacterial abundance and community were highly interrelated with soil chemical properties. The results provided a better understanding of the mechanisms underlying the plant-soil microbe interactions and their responses to biochar dosages in low fertility soil regions.

Maize (Zea mays L.) Seedlings Rhizosphere Microbial Community as Responded to Acidic Biochar Amendment Under Saline Conditions

“… Liao et al (2016) revealed that biochar treatments were different and separated when compared with control due to a higher rate of biochar (4.5 t ha –1 ). The results agree with the results of Luo et al (2020) , who revealed that biochar affected microbial community structure. Similarly, Luo et al (2017) showed that higher biochar treatment increased phospholipid fatty acid profile score.…”

Increase in potato yield by the combined application of biochar and organic fertilizer: key role of rhizosphere microbial diversity

“…The remaining 50 per cent of the nitrogen fertilizer is spread in four equal portions around the roots of potato plants on 15 March, 15 April, 15 May, and 15 June each year, after which the soil was turned over and covered. The proposed dosage of biochar and chemical fertilizer was 2 t·ha -1 ( Luo, 2021 ), N 150 kg·ha -1 , P 2 O 5 135 kg·ha -1 , and K 2 O 135 kg·ha -1 ( Zhang et al., 2017 ), respectively. The nitrogen fertilizer used in the experiment was urea (N 460 g·kg -1 ).…”

Effect of Mangrove Biochar Residue Amended Shrimp Pond Sediment on Nitrogen Adsorption and Leaching

“…The increase in nitrifying bacteria due to the addition of biochar to soil affects moisture content, hydraulic properties, and soil aeration [8]. Furthermore, the porous structure of biochar may provide the habitat for microorganisms, and biochar can release labile C that microbial can use as an energy source [76]. In addition, based on the results of nitrogen leaching in Figures 8 and 9, the application of biochar can increase water-holding capacity and reduce N leaching, which provides nutrient bioavailability for microbial growth.…”