Bionutrients play a vital role in enhancing soil productivity and sustainable agricultural production. In vegetable crops, limited information is available on the relevance of bionutrients in solanaceous crops under protected conditions. Therefore, an experiment was planned to study the response of bionutrients under the modified naturally ventilated polyhouse in mid-hill conditions of Himachal Pradesh for two consecutive years. Various bell pepper varieties, viz., Mekong, Orobelle, Indra and DPCY1, were subjected to a set of bionutrient sources (beejamrit, ghanjeevamrit, jeevamrit and mulching). The results showed that there was a substantial increase in yield parameters in the treatment module, i.e., Mekong + beejamrit@ 200 ml/kg + ghanjeevamrit@5q/ha + jeevamrit @ 500 lt/ha at 21-day intervals + mulching @ 10 t/ha. This treatment exhibited a minimum number of days to 50% flowering (24.16), maximum number of marketable fruits per plant (28.40), fruit length (7.68 cm), fruit breadth (7.70 cm), pericarp thickness (9.15 mm), average fruit weight (109.53 g), plant height (84.06 cm) and marketable yield per plant (3.11 kg). However, Mekong + beejamrit @ 200 ml/kg + ghanjeevamrit @5q/ha + jeevamrit@ 500 lt/ha at 28-day intervals + mulching @ 10 t/hattreatment proved best for total soluble solids (4.58 °Brix), ascorbic acid (166.50 mg/100 g), capsaicin content (6.64%) and carotenoid content (2.43 mg/100 g). Horticultural and biochemical traits were appreciably enhanced after bionutrient application in bell pepper. Therefore, outcomes from the study point out that it is a feasible and economical approach for farmers.