Response of Bovine Cumulus–Oocytes Complexes to Energy Pathway Inhibition during In Vitro Maturation

Lipińska, Paulina; Sell‐Kubiak, Ewa; Pawlak, Piotr; Madeja, Zofia E.; Warzych, Ewelina

doi:10.3390/genes12060838

Cited by 8 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Porcine LD tend to be bigger but fewer compared to bovines. We suspect that this is a species-specific trait, since similar data have been previously published on intracellular LD of porcine (Lee et al, 2019) and bovine (Lipinska et al, 2021) oocytes.…”

Section: Discussionsupporting

confidence: 69%

Species and embryo genome origin affect lipid droplets in preimplantation embryos

Lipińska

Pawlak

Warzych

2023

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Mammalian embryo development is affected by multiple metabolism processes, among which energy metabolism seems to be crucial. Therefore the ability and the scale of lipids storage in different preimplantation stages might affect embryos quality. The aim of the present studies was to show a complex characterization of lipid droplets (LD) during subsequent embryo developmental stages. It was performed on two species (bovine and porcine) as well as on embryos with different embryo origin [after in vitro fertilization (IVF) and after parthenogenetic activation (PA)]. Embryos after IVF/PA were collected at precise time points of development at the following stages: zygote, 2-cell, 4-cell, 8/16-cell, morula, early blastocyst, expanded blastocyst. LD were stained with BODIPY 493/503 dye, embryos were visualized under a confocal microscope and images were analyzed with the ImageJ Fiji software. The following parameters were analyzed: lipid content, LD number, LD size and LD area within the total embryo. The most important results show that lipid parameters in the IVF vs. PA bovine embryos differ at the most crucial moments of embryonic development (zygote, 8–16-cell, blastocyst), indicating possible dysregulations of lipid metabolism in PA embryos. When bovine vs. porcine species are compared, we observe higher lipid content around EGA stage and lower lipid content at the blastocyst stage for bovine embryos, which indicates different demand for energy depending on the species. We conclude that lipid droplets parameters significantly differ among developmental stages and between species but also can be affected by the genome origin.

show abstract

Section: Discussionsupporting

confidence: 69%

Species and embryo genome origin affect lipid droplets in preimplantation embryos

Lipińska

Pawlak

Warzych

2023

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…GAPDH is a gene involved in cell metabolism, impacting cell energy production [28]. Recent studies demonstrated that addition of iodoacetate, a GAPDH inhibitor, during IVM could influence in embryo development, through a lower rate of cleavage and blastocyst, demonstrating the importance of the glucose metabolism mediated by GAPDH [29]. For this, the observed results in cumulus cells are possibly associated to a low cell metabolism during IVM.…”

Section: Discussionmentioning

confidence: 99%

Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system

et al. 2023

View full text Add to dashboard Cite

Despite the advances in in vitro embryo production (IVP) over the years, the technique still has limitations that need to be overcome. In cell cultures, it is already well established that three-dimensional culture techniques are more physiological and similar to the in vivo development. Liquid marble (LM) is a three-dimensional system based on the use of a hydrophobic substance to create in vitro microbioreactors. Thus, we hypothesized that the LM system improves bovine in vitro oocyte maturation and embryo culture. In experiment I, bovine cumulus-oocyte complexes (COCs) were placed for in vitro maturation for 22h in two different groups: control (conventional 2D culture) and LM (three-dimensional culture). We found that oocyte nuclear maturation was not altered by the LM system, however it was observed a decrease in expression of genes important in the oocyte maturation process in cumulus cells of LM group (BCL2, EIF4E, and GAPDH). In experiment II, the COCs were conventionally matured and fertilized, and for culture, they were divided into LM or control groups. There was a decrease in blastocyst rate and cell counting, a down-regulation of miR-615 expression, and an increase in the DNA global methylation and hydroxymethylation in embryos of LM group. Therefore, for the bovine in vitro embryo production, this specific three-dimensional system did not present the advantages that we expected, but demonstrated that the embryos changed their development and epigenetics according to the culture system.

show abstract

“…IA is a pharmacological modulator responsible for inhibiting the glycolytic pathway by the inactivation of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme. It has been reported that supplementation with both IA and DHEA (another glycolytic pathway inhibitor) during bovine IVM resulted in a decrease in blastocyst rates (Lipinska, et al, 2021). It is also known that the decrease in glycolytic pathway activity affects the quality of oocyte maturation (Melanie, et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

The central role of pyruvate metabolism on the epigenetic and molecular maturation of bovine cumulus-oocytes complexes

Silva

Ispada

Junior

et al. 2022

Preprint

View full text Add to dashboard Cite

Pyruvate, the end-product of glycolysis in aerobic conditions, is produced by cumulus cells, and is converted in Acetyl-CoA into the mitochondria of both cumulus cells (CCs) and oocytes as a master fuel input for the tricarboxylic acid cycle (TCA). The citrate generated in the TCA cycle can be directed to the cytoplasm and converted back to acetyl-CoA, being driven to lipid synthesis or, still, being used as the substrate for histones acetylation. This work aimed to verify the impact of pyruvate metabolism on the dynamic of lysine 9 histone 3 acetylation (H3K9ac) and RNA transcription in bovine cumulus-oocyte complexes during in vitro maturation (IVM). Bovine oocytes were IVM for 24h in three experimental groups: Control [IVM medium], sodium dichloroacetate [DCA, a stimulator of pyruvate oxidation in acetyl-CoA] or sodium iodoacetate [IA, a glycolysis inhibitor]. Our results show that both treatments change the metabolic profile of oocytes and CCs, stimulating the use of lipids for energy metabolism in the gamete. This leads to changes in the dynamics of H3K9ac during the IVM in both oocytes and CCs with impact on the synthesis of new transcripts in CCs. A total of 148 and 356 differentially expressed genes were identified in DCA and IA oocytes groups, respectively, when compared to the control group. In conclusion, disorders in pyruvate metabolism during maturation stimulate the beta-oxidation pathway, altering the mitochondrial metabolism, with consequences for the mRNA content of bovine oocyte

show abstract

Response of Bovine Cumulus–Oocytes Complexes to Energy Pathway Inhibition during In Vitro Maturation

Cited by 8 publications

References 37 publications

Species and embryo genome origin affect lipid droplets in preimplantation embryos

Species and embryo genome origin affect lipid droplets in preimplantation embryos

Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system

The central role of pyruvate metabolism on the epigenetic and molecular maturation of bovine cumulus-oocytes complexes

Contact Info

Product

Resources

About