Response of Dairy Cows Treated with Bovine Somatotropin to a Luteolytic Dose of Prostaglandin F2

Kirby, Crystal J.; Wilson, S.J.; Lucy, M.C.

doi:10.3168/jds.s0022-0302(97)75936-8

Cited by 37 publications

(26 citation statements)

References 28 publications

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“…Cattle in negative energy balance typically have increased concentrations of GH (Armstrong et al, 1993;Bossis et al, 1999) due to decreased hepatic binding of GH (Keisler and Lucy, 1996). Reduction of the number of mounts received during estrus in bST-treated cows in the current experiment may be an evolutionary mechanism that decreases reproduction in undernourished cows (Kirby et al, 1997b).…”

Section: Discussionmentioning

confidence: 72%

“…Estrous detection rate decreased linearly as concentration of bST administered to primiparous dairy cows increased (Morbeck et al, 1991), and multiparous dairy cows treated with bST had increased behavioral anestrus (Esteban et al, 1994). Further, Kirby et al (1997b) reported an increased inci-dence of undetectable estrus in dairy cows treated with bST; however, ovulation rate was not altered by bST. In the current study, duration of estrus, as well as number of mounts received, tended to be inversely correlated with concentrations of GH before the breeding season.…”

Section: Discussionmentioning

confidence: 95%

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Influence of body condition and bovine somatotropin on estrous behavior, reproductive performance, and concentrations of serum somatotropin and plasma fatty acids in postpartum Brahman-influenced cows1,2

Flores

Looper

Rorie

et al. 2007

Journal of Animal Science

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Ninety-nine multiparous Brahman-influenced (1/4 to 3/8 Brahman) cows were managed to achieve low (BCS = 4.3 +/- 0.1; n = 50) or moderate (BCS = 6.1 +/- 0.1; n = 49) body condition (BC) to determine the influence of bovine somatotropin (bST) on estrous characteristics, reproductive performance, and concentrations of serum GH and plasma NEFA. Beginning 32 d postpartum, cows within each BC were assigned randomly to treatment with or without bST. Non-bST-treated cows received no treatment, and treated cows were administered bST (Posilac, 500 mg s.c.) on d -35, -21, and -7 before initiation of the breeding season. On d -7, all cows received an intravaginal, controlled internal drug-releasing (CIDR) device. On d 0 (initiation of the 70-d breeding season), the CIDR were removed and cows received prostaglandin F2alpha (PGF2alpha). Blood samples were collected from the median caudal vein of the cows at each bST treatment and at d -28 and 0. Estrous behavior was monitored by radiotelemetry during the first 30 d of the breeding season. Growth hormone was increased (P < 0.05) in low and moderate BC cows treated with bST. The percentage of cows detected in estrus during the first 30 d of the breeding season was decreased (P = 0.05) for low BC (64%) compared with moderate BC (82%) cows. The interval to first estrus tended (P = 0.07) to be shorter in low BC-bST-treated cows (3.7 +/- 1.9 d) than in moderate BC-bST-treated cows (9.6 +/- 1.8 d). During the first 30 d of the breeding season, cows in low BC had a decreased (P = 0.02) number of mounts received and increased (P = 0.001) quiescence between mounts compared with cows in moderate BC. The number of mounts received was reduced (P = 0.04) in bST-treated cows. More (P = 0.02) cows treated with bST became pregnant during the first 3 d of the breeding season compared with non-bST-treated cows. The cumulative first-service conception rate tended (P = 0.07) to be greater for bST-low BC cows than non-bST-treated cows in low or moderate BC. On d 0, NEFA were greater (P < 0.05) in bST-treated vs. non-bST-treated cows. Low BC and bST reduced the intensity of behavioral estrus in postpartum Brahman-influenced cows. However, bST increased the first-service conception rate during the first 30 d of breeding and pregnancy rates during the first 3 d of breeding in postpartum Brahman-influenced cows.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 95%

Influence of body condition and bovine somatotropin on estrous behavior, reproductive performance, and concentrations of serum somatotropin and plasma fatty acids in postpartum Brahman-influenced cows1,2

Flores

Looper

Rorie

et al. 2007

Journal of Animal Science

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“…Serum progesterone concentrations were quantified by radioimmunoassay with a Coat‐a‐Count RIA kit (Diagnostic Products Corporation, Los Angeles, CA), as described previously by Kirby, Wilson, and Lucy (). Intra‐assay coefficients of variations were 4.82%, and the assay sensitivity was 0.08 ng/ml for the progesterone assay.…”

Section: Methodsmentioning

confidence: 99%

“…Serum progesterone concentrations were quantified by radioimmunoassay with a Coat-a-Count RIA kit (Diagnostic Products Corporation, Los Angeles, CA), as described previously by Kirby, Wilson, and Lucy (1997…”

Section: Progesterone Assaysmentioning

confidence: 99%

Circulating microRNA as candidates for early embryonic viability in cattle

Pohler¹,

Green

Moley

et al. 2017

Molecular Reproduction Devel

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Blood-borne extracellular vesicles (i.e. exosomes and microvesicles) carrying microRNAs (miRNAs) could make excellent biomarkers of disease and different physiologic states, including pregnancy status. We tested the hypothesis that circulating extracellular vesicle-derived miRNAs might differentiate the pregnancy status of cows that had maintained pregnancy to Day 30 from non-pregnant cows or from those that exhibited embryonic mortality between Days 17–30 of gestation. Cows were randomly assigned for artificial insemination with fertile semen (n= 36) or dead semen (n=8; control group) on Day 0 (day of estrus). Blood was collected from all animals on Day 0 and on Days 17 and 24 after artificial insemination. Cows receiving live sperm were retrospectively classified as pregnant on Day 30 (n=17) or exhibiting embryonic mortality between Days 17–30 (n=19). Extracellular vesicles from Day-17 and -24 samples were isolated from serum using ultra-centrifugation, and their presence was confirmed by nanoparticle tracking and Western blot analyses (for CD81) prior to RNA extraction. MicroRNA sequencing was performed on pregnant, embryonic-mortality, and control cows (n=4 per day), for a total of 24 independent reactions. In total, 214 miRNAs were identified in serum, 40 of which were novel. Based on differential abundance parameters, we identified 32 differentially abundant loci, representing 27 differentially abundant mature miRNA. At Days 17 and 24, specific miRNAs (e.g. miR-25, -16b and -3596) were identified that differentiated the pregnancy status. In summary, we identified several circulating extracellular vesicles derived miRNAs that differ in abundance between embryonic mortality and pregnant cows.

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“…Entretanto, pesquisadores brasileiros como BURATINI JR et al (2000) verificaram aumento significativo no número de folículos recrutados na segunda onda, atribuindo este aumento à somatotropina, aumento este que pode estar relacionado ao aumento da concentração plasmática de IGF-I como encontrado por GONG et al (1991), GONG et al (1993), LUCY et al (1993), HERRLER et al (1994, DE LA SOTA et al (1996), KIRBY et al (1997), os quais obtiveram os mesmos resultados, relacionando nesse contexto a IGF-I, implicada no mecanismo do crescimento folicular.…”

Section: Discussionunclassified

A SOMATOTROPINA RECOMBINANTE BOVINA (bST) E A DINÂMICA FOLICULAR EM BOVINOS LEITEIROS

Aleixo¹,

Kozicki²,

Weiss³

et al. 2005

AVS

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RESUMO -O trabalho objetivou determinar a influência da somatotropina recombinante bovina (bST) sobre a reprodução de vacas da raça Holandesa Preta e Branca. Durante um ciclo estral, verificou-se via ultrassonografia, a dinâmica folicular acompanhando-se particularmente o diâmetro dos ovários, o número de ondas foliculares durante o ciclo estral, a duração de cada onda folicular, o número e diâmetro dos folículos recrutados. Foram utilizadas doze fêmeas, sendo seis do grupo controle e seis do grupo tratado, escolhidas ao acaso. O tratamento foi executado mediante administração de uma dose de somatotropina recombinante bovina no sexagésimo dia post partum (pp). A dinâmica folicular dos ovários foi acompanhada por exames ultrassonográficos diários a partir do dia o (estro base e visível) após o parto, até que os animais manifestassem novo cio. No 60º dia os animais do grupo tratado receberam uma injeção de bST (500 mg, SC) e os animais do grupo controle, 1,4 ml de solução fisiológica estéril (SC), como placebo. Para o monitoramento ovariano dos animais, foi utilizado aparelho de ultrassonografia com transdutor de 5 MHz. Concluiu-se que a somatotropina recombinante bovina administrada no 2º mês pp, não exerceu significativa influência sobre a atividade ovariana dos animais, muito embora tenha havido aumento da produção leiteira diária em 3,5% durante o período pesquisado. Os estudos da dinâmica folicular e a somatotropina recombinante bovina não alteraram, o comprimento do ciclo estral, o diâmetro dos ovários, o número de ondas foliculares, o comprimento de cada onda, o número e o diâmetro dos folículos recrutados.Palavras-chaves: somatotrofina recombinante bovina, vacas leiteiras, ultrassonografia.ABSTRACT -The aim of the present research was to determine the influence of the recombinant bovine somatotropin (bST) on the reproduction of Black and White Holstein bovine females being evaluated along a complete estrous cycle. The follicular dynamics has been observed in regard to the diameter of the ovaries, number of follicular waves during the estrous cycle, duration of each follicular wave, number and diameter of the recruited follicles. Twelve females were used for the experiment, being six of the control group and six of the treated group, chosen at random. The treatment was carried out by administration of a dose of bovine recombinant somatotropin in the sixtieth day post partum (pp). The ovaries follicular dynamics was followed by daily ultrasound exams starting from the day of the first estrus base (visible estrus, considered the 0 day) post partum, until the animals manifested new estrous. In the 60th day the animals of the treated group received an injection of bST (500 mg, SC) and the animals of the group control, 1,4 ml of physiologic solution (SC), as placebo. For the ovarian monitoring of the animals, ultrasound apparel was used with transducer of 5 MHz. It has been observed that the bovine recombinant somatotropin administered in the 2nd month pp, did not display significant influence on the ovarian act...

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Response of Dairy Cows Treated with Bovine Somatotropin to a Luteolytic Dose of Prostaglandin F2

Cited by 37 publications

References 28 publications

Influence of body condition and bovine somatotropin on estrous behavior, reproductive performance, and concentrations of serum somatotropin and plasma fatty acids in postpartum Brahman-influenced cows1,2

Influence of body condition and bovine somatotropin on estrous behavior, reproductive performance, and concentrations of serum somatotropin and plasma fatty acids in postpartum Brahman-influenced cows1,2

Circulating microRNA as candidates for early embryonic viability in cattle

A SOMATOTROPINA RECOMBINANTE BOVINA (bST) E A DINÂMICA FOLICULAR EM BOVINOS LEITEIROS

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