A field trial to determine the effect of different dosages, application time of plant growth regulators and defoliators on growth and yield of cotton under high density planting system was conducted at Regional Agricultural Research Station, Warangal during kharif-2022. The experiment consisted of eleven treatments laid out in Randomized Block Design with three replications. The treatment details were T1: Application of Mepiquat chloride (MC) 25g a.i ha-1 @40&55 DAE+ Etherel 2000ppm @40% boll burst, T2: MC 25 g a.i ha-1 @40&55 DAE+ Etherel 2000ppm @60% boll burst, T3: MC 20g a.i ha-1 @40DAE, MC 30g a.i ha-1 @60DAE+ Etherel 2000ppm@ 40% boll burst T4: MC 20g a.i ha-1 @40DAE, MC 30g a.i ha-1 @60DAE+ Etherel 2000ppm@ 60% boll burst T5: MC 20 g a.i ha-1 @40,55 & 75 DAE+ Etherel 2000ppm @40% boll burst, T6: MC 20 g a.i ha-1 @40,55 & 75 DAE+ Etherel 2000ppm @40% boll burst, T7: MC 25 g a.i ha-1 @4 0,55 & 75 DAE+ Etherel 2000ppm @40% boll burst, T8: MC 25 g a.i ha-1 @40,55 & 75 DAE+ Etherel 2000ppm @60% boll burst, T9: 20g a.i ha-1@ 40DAE, 25g a.i ha-1@55DAE & 30g a.i ha-1@70DAE +Etherel 2000ppm@ 40% boll burst, T10: 20g a.i ha-1@ 40DAE, 25g a.i ha-1@55DAE & 30g a.i ha-1@70DAE +Etherel 2000ppm@ 60% boll burst and T11: Control. Results revealed that foliar application of MC @ 20 g a.i ha-1@ 40,55 & 70DAE in conjunction with Etherel 2000ppm @60% boll burst recorded higher seed cotton yield. Plant growth, dry matter accumulation and stalk yield were recorded highest in control plot and lowest with spraying of : 20g a.i ha-1@ 40DAE, 25g a.i ha-1@55DAE & 30g a.i ha-1@70DAE +Etherel 2000ppm@ 40% boll burst. Among Mepiquat chloride treatments with similar doses, all the agronomic traits of cotton crop are positively influenced with application of 2000ppm of etherel @60% boll burst. The spraying of M.C @ 20 g a.i ha-1 at 40,55 and 70 DAE + Etherel 2000ppm @ 60% boll burst would be economically ideal to the farming community.