Response of Durum Wheat Seedlings to Salinity

Yıldırım, Mehmet; Kızılgeçi, Ferhat; Akıncı, Cuma; Albayrak, Önder

doi:10.15835/nbha4319708

Cited by 6 publications

(5 citation statements)

References 13 publications

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“…These results agree with previous observations, which showed that osmotic stress and salinity in the immediate environment of germinating durum wheat seeds can delay or inhibit the germination process [41,42] and reduce the early seedling growth [43][44][45]. The use of a mannitol solution iso-osmotic to the NaCl solution allows to evaluate the relative contribution of the two components of the salt stress, namely the osmotic stress and the ion toxicity.…”

Section: Growth Performances Of Seedlings Grown Under Control Mannitol and Nacl Stress Conditionssupporting

confidence: 91%

The Molecular and Functional Characterization of the Durum Wheat Lipoxygenase TdLOX2 Suggests Its Role in Hyperosmotic Stress Response

Menga

Trono

2020

Plants

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In plants, lipoxygenases (LOXs) are involved in various processes, such as growth, development, and response to stress cues. In the present study, the expression pattern of six durum wheat LOX-encoding genes (TdLpx-B1.1, TdLpx-B1.2, TdLpx-A2, TdLpx-B2, TdLpx-A3 and TdLpx-B3) under hyperosmotic stress was investigated. With osmotic (0.42 M mannitol) and salt (0.21 M NaCl) stress imposed at the early stages of seedling growth, a strong induction of the TdLpx-A2 gene expression in the shoots paralleled an equally strong increase in the LOX activity. Enhanced levels of malondialdehyde (MDA) and increased rates of superoxide anion generation were also observed as a result of the stress imposition. Sequence analysis of the TdLOX2 encoded by the TdLpx-A2 gene revealed that it belonged to the type-1 9-LOX group. When overexpressed in E. coli, TdLOX2 exhibited normal enzyme activity, high sensitivity to specific LOX inhibitors, with 76% and 99% inhibition by salicylhydroxamic and propyl gallate, respectively, and a preference for linoleic acid as substrate, which was converted exclusively to its corresponding 13-hydroperoxide. This unexpected positional specificity could be related to the unusual TV/K motif that in TdLOX2 replaces the canonical TV/R motif of 9-LOXs. Treatment of seedlings with propyl gallate strongly suppressed the increase in LOX activity induced by the hyperosmotic stress; the MDA accumulation was also reduced but less markedly, whereas the rate of superoxide anion generation was even more increased. Overall, our findings suggest that the up-regulation of the TdLpx-A2 gene is a component of the durum wheat response to hyperosmotic stress and that TdLOX2 may act by counteracting the excessive generation of harmful reactive oxygen species responsible for the oxidative damages that occur in plants under stress.

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Section: Growth Performances Of Seedlings Grown Under Control Mannitol and Nacl Stress Conditionssupporting

confidence: 91%

The Molecular and Functional Characterization of the Durum Wheat Lipoxygenase TdLOX2 Suggests Its Role in Hyperosmotic Stress Response

Menga

Trono

2020

Plants

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“…Previously, root length and seedling growth of wheat cultivars were drastically reduced with increasing NaCl stress (Khatun et al, 2013). Yildirim et al (2015) noted that root length could serve as reliable criteria for determining the salinity tolerance of crops genotypes, as roots have direct contact with soil and are responsible for absorption of nutrients and water.…”

Section: Root Length (Cm)mentioning

confidence: 99%

Comparative study on germination and seedling growth of wheat cultivars under salt stress regimes

Iyem

Yıldırım

Kızılgeçi

2020

International Journal of Agriculture Environment and Food Sciences

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Salinity interferes with germination and hampers the growth of wheat especially at seedling stage which necessitates determining of salt tolerant cultivars. Based upon the current situation a controlled experiment was carried out at the Laboratory of Horticulture, Faculty of Agriculture, Cukurova University, Turkey to comparatively evaluate wheat varieties response to imposed salt stress. Germination and seedling growth properties under salt stress were taken as response variables. The seeds of five bread wheat genotypes ('Wafia', 'Lucilla', 'Envoy', 'Lok1'and 'RSP-561') were placed in Petri dishes with salinity doses (Control (0), 4, 8 and 12 dS.m-1 NaCl) which were applied at germination and subsequent early seedling phases under laboratory conditions. The results revealed that root growth was highly sensitive to salt stress and the varieties of Envoy and Lucilla remained relatively tolerant to salt stress than other cultivars. The biochemical analysis revealed that proline content spiked with increasing salinity level, 'RSP-561' under 8 dS.m-1 and 12 dS.m-1 recorded the maximum proline content. Salt stress boosted leaf proline content of salt sensitive wheat genotypes ('Wafia' and 'Lok1'), whereas declined proline level was observed for salt tolerant cultivars. In addition, salt-sensitive genotypes showed a reduction in chlorophyll content a, b, total chlorophyll and carotenoid while, 'Wafia' and 'RSP-561' recorded the minimum Chlorophylls and Carotenoid contents. Further investigations are needed, however, to enhance understanding of the salt stress effects during the whole growing cycle of wheat.

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“…Twenty seeds of each wheat genotype were planted in each petri dishes containing filter paper. Germination rate, seedling vigor, coleoptile length, root length and shoot length determined following the method of Yildirim et al [7]. Analysis of variance was performed using the Genstat statistic package.…”

Section: Methodsmentioning

confidence: 99%

The Drought Effect on Seed Germination and Seedling Growth in Bread Wheat (Triticum aestivum L.)

Kızılgeçi

Tazebay²,

Namlı³

et al. 2017

International Journal of Agriculture Environment and Food Sciences

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Drought is a major abiotic stress that threating the producing and survival of many crops such as cereal. Wheat is grown on arid lands and drought often causes serious problem in wheat production on these fields. In this study, it was determined that the effect of five different drought stress (0, -0.3, -0.6, -0.9, -1.2 MPa, by using PEG 6000 solution) on the germination of 4 different bread wheat cultivars (Tekin, Pehlivan, DZT13-1 and DZT13-2). The study was carried out according to completely randomized design in factorial arrangement with four replications. In the present study, important growth parameters like germination rate (%), seedling vigor (%), coleoptile length (cm), root length (cm) and shoot length (cm) were observed. Results showed when dose of PEG increased, seedling growth was significantly affected. Increasing drought stress was resulted with a decrease on germination rate, seedling vigor, coleoptile length, root length and shoot length.

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Response of Durum Wheat Seedlings to Salinity

Cited by 6 publications

References 13 publications

The Molecular and Functional Characterization of the Durum Wheat Lipoxygenase TdLOX2 Suggests Its Role in Hyperosmotic Stress Response

The Molecular and Functional Characterization of the Durum Wheat Lipoxygenase TdLOX2 Suggests Its Role in Hyperosmotic Stress Response

Comparative study on germination and seedling growth of wheat cultivars under salt stress regimes

The Drought Effect on Seed Germination and Seedling Growth in Bread Wheat (Triticum aestivum L.)

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