Response of Enteromorpha sp. (Chlorophyceae) to a nitrate pulse: nitrate uptake, inorganic nitrogen storage and nitrate reductase activity

Lartigue, Julien; Sherman, Timothy D.

doi:10.3354/meps292147

Cited by 35 publications

(34 citation statements)

References 55 publications

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“…In Gracilaria chilensis, NR activity was thus rapidly stimulated within a few minutes (Chow et al, 2007;Chow & Oliveira, 2008), probably by post-translational NR-protein regulation. Lartigue & Sherman (2005) observed the same trend in Enteromorpha sp. A like inducing response, under the same conditions, has also been observed in other macroalgae (Gao, Smith & Alberte 1995;Lartigue & Sherman, 2005;Young et al, 2007;Martins et al, 2009;Cabello-Pasini et al, 2011).…”

Section: Nitrate Reductase In Macroalgaesupporting

confidence: 64%

“…Lartigue & Sherman (2005) observed the same trend in Enteromorpha sp. A like inducing response, under the same conditions, has also been observed in other macroalgae (Gao, Smith & Alberte 1995;Lartigue & Sherman, 2005;Young et al, 2007;Martins et al, 2009;Cabello-Pasini et al, 2011). Nitrate reductase activity in Arctic species appears to be directly enhanced by nitrate addition, with relatively little feedback from the N-status of the cell (Gordillo et al, 2006).…”

Section: Nitrate Reductase In Macroalgaesupporting

confidence: 64%

“…The use of NR in an ecological context is particularly relevant for marine environments where nitrogen is often limiting, thereby providing relevant information regarding the physiological nitrogen status of organisms (Hernández et al, 1993). Nitrate reductase is considered as a key enzyme in nitrogen metabolism, through being, not only the rate-limiting enzyme in inorganic nitrogen assimilation, but also the major regulatory step in nitrogen metabolism (Crawford, 1995;Berges, 1997;Lartigue & Sherman, 2005;Young et al, 2009). Changes in NR activity, both in the field or in laboratory, have been examined in very few macroalgae.…”

Section: Nitrate Reductase In Macroalgaementioning

confidence: 99%

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Nitrate Assimilation: The Role of In Vitro Nitrate Reductase Assay as Nutritional Predictor

Chow¹

2012

Applied Photosynthesis

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Section: Nitrate Reductase In Macroalgaesupporting

confidence: 64%

Section: Nitrate Reductase In Macroalgaesupporting

confidence: 64%

Section: Nitrate Reductase In Macroalgaementioning

confidence: 99%

See 1 more Smart Citation

Nitrate Assimilation: The Role of In Vitro Nitrate Reductase Assay as Nutritional Predictor

Chow¹

2012

Applied Photosynthesis

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“…This difference may be due to the nitrate's negative charge, which makes the uptake energy-dependent and thus slower (Runcie et al, 2003). After the uptake, nitrate must be converted into ammonium in a rate-limiting step catalyzed by nitrate reductase (NR) before being accumulated in vacuoles (Viaroli et al, 1996;Cohen and Fong, 2004;Lartigue and Sherman, 2005). The level of NR in Ulva intestinalis appears to be determined by the presence or absence of NO 3 À in the water column conferring macroalgae the ability to use a greater proportion of this nitrogen form.…”

Section: Discussionmentioning

confidence: 99%

The response of primary producer assemblages to mitigation measures to reduce eutrophication in a temperate estuary

Leston

Lillebø

Pardal

2008

Estuarine, Coastal and Shelf Science

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The Mondego estuary is a well-described system located on the North Atlantic Ocean, where cultural eutrophication progressed over the last decades of the 20th century. Consequently, and due to a large productivity of Ulva spp., Zostera noltii meadows were severely reduced with a concomitant decrease in environmental quality. In 1998, experimental mitigation measures were implemented, via changes in hydrology to increase circulation and diversion of nutrient-rich freshwater inflow, to reverse the process in the most affected area of the estuary e its South arm.The objective of this study was to assess the differences in response of primary producer assemblages to the implemented measures to reduce eutrophication.Results show that the mean concentrations of DIN suffered a notorious decrease due to a significant reduction in the ammonium concentration in the water column, while DIP increased significantly. Primary producer assemblages showed different responses to these changes: phytoplankton, measured as concentration of chlorophyll a, did not show any significant changes; green macroalgae, mostly Ulva spp., suffered a large reduction in biomass, whereas Gracilaria gracilis and the macrophyte Zostera noltii biomasses increased greatly. Results show that phytoplankton biomass has remained constant and suggest that the reduction in ammonium could have been responsible for the changes in the green macroalgal biomass. Light was the most likely factor in the response of seagrass whereas red macroalgal reaction seemed to be dependent on both light and ammonium.

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“…These nutrients are mainly provided in the form of transient pulses. However, the physiological response may depend greatly on the nutrient storage capability of the species at short time scales (Vergara et al, 1995;McGlathery, 1996;Pedersen and Borum, 1996;Costanzo et al, 2000;Nielsen, 2003;Bracken, 2004;Clavier et al, 2005;Lartigue and Sherman, 2005).…”

Section: Introductionmentioning

confidence: 99%

Environmental control of the annual erect phase of <i>Nemalion helminthoides</i> (Rhodophyta) in the field

Pato¹,

Martínez²,

Ordás³

2010

Sci. Mar.

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SUMMARY: In temperate areas, lack of nutrients during summer, particularly N, is the main limiting factor of macroalgal growth. However, Nemalion helminthoides (Velley) Batters in northern Spain is conspicuous in the field during this time (from mid-May to late-July). Therefore, we assumed that its nutrient requirements are low enough to be sustained by transient nutrient inputs and we hypothesized that the physiological condition of the thalli was transiently improved when nutrient pulses occurred. A range of proxies for physiological condition (internal N, C, proteins and phycobilins), growth and phenological status of N. helminthoides were measured over time and related to temporal variations in nutrient availability, irradiance, temperature and daylength. Data were analyzed using a multivariate approach (redundancy analysis). Transient nutrient inputs were mainly due to freshwater runoff and wind-driven upwelling events; however, these pulses did not lead to any short-term improvement in the physiological condition of the algae because in such dominant nutrient limiting conditions plants divert transient available resources directly to growth and reproduction. Probably because of the strong endogenous nature of the N. helminthoides life-history, only daylength and temperature were found to be major environmental factors: increasing daylength was associated with growth, sexual maturation, fertilization and the increment of internal N and C, the amount of proteins and phycobilins. Decreasing daylength together with increasing temperature were correlated with spore release and senescence. This research suggests that N. helminthoides requires a high light dose to sustain growth and reproduction, and therefore it must grow and reproduce in summer even though it has to overcome nutrient deprivation during this period.Keywords: carbon, daylength, multivariate, Nemalion helminthoides, nitrogen, phenology, phycobilins, protein, RDA, temperature.RESUMEN: Control ambiental de la fase macroscópica de NemalioN helmiNthoides (Rhodophyta) en el campo. -En las costas templadas la falta de nutrientes durante el verano, y en particular la falta de N, es la causa principal de la limitación del crecimiento de las algas. Sin embargo en la costa N de España Nemalion helminthoides (Velley) Batters aparece precisamente durante esta época del año, desde mediados de mayo a finales de julio. Por ello se asume que sus requerimientos de nutrientes son lo suficientemente bajos como para aprovechar pulsos ocasionales, y se plantea la hipó-tesis de que la condición fisiológica del talo mejora durante la ocurrencia de estos pulsos. Para comprobar esta hipótesis se midieron indicadores de la condición fisiológica tales como el contenido interno de N, C, proteínas y ficobilinas, además del crecimiento y del estado fenológico a lo largo del periodo de crecimiento estival, que se han relacionado con variaciones temporales en disponibilidad de nutrientes, irradiancia, temperatura y fotoperiodo. Los datos se analizaron utilizando una aproximación...

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Response of Enteromorpha sp. (Chlorophyceae) to a nitrate pulse: nitrate uptake, inorganic nitrogen storage and nitrate reductase activity

Abstract: The uptake, storage and reduction of nitrate (NO 3 -) by Enteromorpha sp. during a NO 3 -

Cited by 35 publications

References 55 publications

Nitrate Assimilation: The Role of In Vitro Nitrate Reductase Assay as Nutritional Predictor

Nitrate Assimilation: The Role of In Vitro Nitrate Reductase Assay as Nutritional Predictor

The response of primary producer assemblages to mitigation measures to reduce eutrophication in a temperate estuary

Environmental control of the annual erect phase of <i>Nemalion helminthoides</i> (Rhodophyta) in the field

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