Response of Faba bean (Vicia faba L.) to combined inoculation with Azotobacter and Rhizobium leguminosarum bv. viceae

Rodelas, B.; González‐López, Jesús; Pozo, Clementina; Salmerón, V.; Martı́nez-Toledo, M.V.

doi:10.1016/s0929-1393(98)00157-7

Cited by 35 publications

(24 citation statements)

References 31 publications

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“…In pot experiments conducted under gnotobiotic conditions or with the use of sterile soils [Burns et al 1981, El-Bahrawy 1983, Rodelas et al, 1999 it has been shown that nodulation intensity, efficiency of nitrogen fixation and yields of some legumes inoculated with mixed cultures of rhizobia and other beneficial microorganisms, like Azotobacter spp. and Azospirillum spp., were higher as compared to those inoculated with the rhizobia alone.…”

Section: Resultsmentioning

confidence: 99%

Symbiotic Effectiveness of Rhizobium Leguminosarum Bv. Viciae With Pea Plants as Influenced by Azotobacter Chroococcum

Martyniuk¹,

Kozieł²,

Gałązka³

2015

J. Ecol. Eng.

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Section: Resultsmentioning

confidence: 99%

Symbiotic Effectiveness of Rhizobium Leguminosarum Bv. Viciae With Pea Plants as Influenced by Azotobacter Chroococcum

Martyniuk¹,

Kozieł²,

Gałązka³

2015

J. Ecol. Eng.

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“…Root attachment and colonization constitute an important stage, and little is known about their mechanisms. A. vinelandii has been known to be a good plant growth-promoting bacterium (2,4). In this study, the response of A. vinelandii to association with the roots of O. sativa was examined under different growth medium conditions.…”

Section: Discussionmentioning

confidence: 99%

“…It is well known to be a plant growth-promoting bacterium capable of fixing nitrogen and forming desiccation-resistant cysts under unfavorable growth condition (1,2). The former activity requires it to house several oxygen-sensitive mechanisms while being an obligate aerobic bacterium (3).…”

mentioning

confidence: 99%

Hypothetical Protein Avin_16040 as the S-Layer Protein of Azotobacter vinelandii and Its Involvement in Plant Root Surface Attachment

Liew

Jong

Najimudin

2015

Appl Environ Microbiol

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A proteomic analysis of a soil-dwelling, plant growth-promoting Azotobacter vinelandii strain showed the presence of a protein encoded by the hypothetical Avin_16040 gene when the bacterial cells were attached to the Oryza sativa root surface. An Avin_16040 deletion mutant demonstrated reduced cellular adherence to the root surface, surface hydrophobicity, and biofilm formation compared to those of the wild type. By atomic force microscopy (AFM) analysis of the cell surface topography, the deletion mutant displayed a cell surface architectural pattern that was different from that of the wild type. Escherichia coli transformed with the wild-type Avin_16040 gene displayed on its cell surface organized motifs which looked like the S-layer monomers of A. vinelandii. The recombinant E. coli also demonstrated enhanced adhesion to the root surface. Azotobacter vinelandii is a Gram-negative free-living and obligate aerobic soil bacterium. It is well known to be a plant growth-promoting bacterium capable of fixing nitrogen and forming desiccation-resistant cysts under unfavorable growth condition (1, 2). The former activity requires it to house several oxygen-sensitive mechanisms while being an obligate aerobic bacterium (3). A. vinelandii also has characteristics such as production of plant growth hormones and antibiotics (4) as well as industrially important substances such as extracellular polysaccharide (EPS) alginate, poly-␤-hydroxybutyrate (PHB), and siderophore compounds (5).Many diverse genera of nitrogen-fixing bacteria are present in the plant rhizosphere. The effectiveness of their plant growthpromoting activity depends upon the establishment of their cells in the rhizosphere. This interaction depends upon many factors, one of them being plant exudates. As a diazotroph, A. vinelandii provides fixed nitrogen to the plant while acquiring sugars and other nutrients that leak from the roots (6).The complete A. vinelandii genome (GenBank accession number NC_012560) has explained many biochemical pathways and structures of the bacterium (7). It has also revealed hypothetical genes with unannotated functions. The advances in proteomic technology have led to new understanding of and insights into many important proteins and their related mechanisms.Studies have shown that plant-microbe communication is a two-way interaction involving various signal molecules that cause metabolic changes in both organisms (8-10). Nevertheless, there is limited information on the plant-bacterium interaction, especially with the roots and the rhizosphere. In this study, a proteomic approach was successfully used to study the interaction between a root-associated bacterium and rice plant in the rhizosphere (11-13).In this study, a differential proteomic analysis of A. vinelandii ATCC 12837 in response to different conditions and at different locations within the Oryza sativa MR 219 rhizosphere was performed. By two-dimensional gel electrophoresis (2DE) followed by tandem mass spectrometry (MS/MS) analyses, several known and hypothetical p...

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“…Both strains are vitamin producers in media amended with 0Á5% glucose, and have shown growth-promoting ability on plants (Martõ Ânez-Toledo et al 1988a, b;Rodelas et al 1999).…”

Section: Strainsmentioning

confidence: 99%

Production of B-group vitamins by twoAzotobacterstrains with phenolic compounds as sole carbon source under diazotrophic and adiazotrophic conditions

Revillas¹,

Rodelas²,

Pozo

et al. 2000

Journal of Applied Microbiology

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Azotobacter vinelandii strain ATCC 12837 and A. chroococcum strain H23 (CECT 4435) were able to grow on N‐free or NH4Cl‐amended chemically‐defined (Burk's) media, with protocatechuic acid (1–2 mmol l−1) or sodium p‐hydroxybenzoate (1–10 mmol l−1) as sole carbon (C) sources. At a concentration of 2 mmol l−1, both substrates supported nitrogen fixation (acetylene reduction assay) at similar or higher rates than bacteria grown in control media amended with 2 mmol l−1 sodium succinate as C source. The two strains produced the B‐group vitamins niacin, pantothenic acid, thiamine, riboflavin and biotin after 72 h of growth in chemically‐defined media with 2 mmol l−1 protocatechuic acid, sodium p‐hydroxybenzoate or sodium succinate as sole C source, either in N‐free media or in media amended with 0·1% NH4Cl. Quantitative production of all vitamins was affected by the use of the different C and N substrates.

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Response of Faba bean (Vicia faba L.) to combined inoculation with Azotobacter and Rhizobium leguminosarum bv. viceae

Cited by 35 publications

References 31 publications

Symbiotic Effectiveness of Rhizobium Leguminosarum Bv. Viciae With Pea Plants as Influenced by Azotobacter Chroococcum

Symbiotic Effectiveness of Rhizobium Leguminosarum Bv. Viciae With Pea Plants as Influenced by Azotobacter Chroococcum

Hypothetical Protein Avin_16040 as the S-Layer Protein of Azotobacter vinelandii and Its Involvement in Plant Root Surface Attachment

Production of B-group vitamins by twoAzotobacterstrains with phenolic compounds as sole carbon source under diazotrophic and adiazotrophic conditions

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