Response of fungal endophyte communities within Andropogon gerardii (Big bluestem) to nutrient addition and herbivore exclusion

Watson, Monica; Bushley, Kathryn E.; Seabloom, Eric W.; May, Georgiana

doi:10.1016/j.funeco.2021.101043

Cited by 3 publications

(6 citation statements)

References 90 publications

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“…The 476 sequences clustered into 82 OTUs at 97% similarity, and of those, 45 OTUs (9.5% of total sequences) were represented by only one culture (singletons). A high fraction of OTUs represented only once in the sample suggests communities comprised of few abundant and many rare taxa, as previously observed for A. gerardii [68]. Most of the isolates obtained belonged to the Phylum Ascomycota (73 of 82 OTUs; 98% of sequences).…”

Section: Fungal Isolation Otu Assignment and Richnesssupporting

confidence: 73%

“…The two isolation methods apparently favor different fungal growth types; the sectioning approach obtained more slow-growing fungi and the leaf maceration approach obtained more fast-growing fungi. While the cause for the differing recovery of growth types is not easily discerned, the recovery of two assemblages exhibiting distinct growth rates was unexpected as they have not previously been reported in similar studies (e.g., [3,12,43,68,89]). More importantly, the slow-growing and fast-growing assemblages are associated with differing patterns of resource use as taxa in the slow-growing assemblage exhibited a lower mean niche width, but greater variation in resource use than fungi in the fast-growing assemblage (Figs 2 and 3).…”

Section: S3 Fig)mentioning

confidence: 53%

“…The experimental plots were established in 2007 and treatments have been subsequently applied annually. The impacts of the NPK+ treatment on foliar endophyte communities have been reported in other studies which show shifts in community composition due to fertilization treatments within sites [65,[67][68][69][70]. Here, we evaluate the functional and taxonomic diversity of fungal endophytes occurring across the NPK+ and Control plots.…”

Section: Study Sitementioning

confidence: 72%

“…The deep evolutionary origins of taxa constituting slow-and fast-growing assemblages is demonstrated by their distinction at the class and order level (Fig 3 ), divergences encompassing over 120-300 million years [98][99][100][101]. Within the Sordariomycetes, a fungal class often represented in endophytic communities of grasses [12,65,68,89,[102][103][104], differences in growth traits characterize taxa belonging to different orders (Fig 3). While our ability to generalize to endophyte communities of all plants is limited by our small sample of A. gerardii leaves, deep evolutionary origins of functional traits have previously been demonstrated in endophytic fungi [41,42,105] and other fungal symbionts of plants as well [106][107][108][109][110].…”

Section: S3 Fig)mentioning

confidence: 99%

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Cryptic functional diversity within a grass mycobiome

Ndinga-Muniania,

Wornson,

Fulcher

et al. 2023

PLoS ONE

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Eukaryotic hosts harbor tremendously diverse microbiomes that affect host fitness and response to environmental challenges. Fungal endophytes are prominent members of plant microbiomes, but we lack information on the diversity in functional traits affecting their interactions with their host and environment. We used two culturing approaches to isolate fungal endophytes associated with the widespread, dominant prairie grass Andropogon gerardii and characterized their taxonomic diversity using rDNA barcode sequencing. A randomly chosen subset of fungi representing the diversity of each leaf was then evaluated for their use of different carbon compound resources and growth on those resources. Applying community phylogenetic analyses, we discovered that these fungal endophyte communities are comprised of phylogenetically distinct assemblages of slow- and fast-growing fungi that differ in their use and growth on differing carbon substrates. Our results demonstrate previously undescribed and cryptic functional diversity in carbon resource use and growth in fungal endophyte communities of A. gerardii.

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Section: Fungal Isolation Otu Assignment and Richnesssupporting

confidence: 73%

Section: S3 Fig)mentioning

confidence: 53%

Section: Study Sitementioning

confidence: 72%

Section: S3 Fig)mentioning

confidence: 99%

See 2 more Smart Citations

Cryptic functional diversity within a grass mycobiome

Ndinga-Muniania,

Wornson,

Fulcher

et al. 2023

PLoS ONE

Self Cite

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“…Nitrogen (N) and phosphorus (P) are two important nutrients for plant growth (Zhang et al, 2017). The imbalanced N and P deposition induced by human activities, such as fertilization and fossil fuel combustion (Delonge et al, 2008), affects the primary production of plants and profoundly influences the structure and function of ecosystems (Sardans et al, 2012;Chen et al, 2015;Monuca et al, 2021). To explore how nutrient cycling between plants and soil in different ecosystems responds to nutrient deposition, researchers conducted many experimental studies (Chen et al, 2015;.…”

Section: Introductionmentioning

confidence: 99%

Effect of nitrogen and phosphorus addition on leaf nutrient concentrations and nutrient resorption efficiency of two dominant alpine grass species

Yue

et al. 2021

J. Arid Land

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Nitrogen (N) and phosphorus (P) are two essential nutrients that determine plant growth and many nutrient cycling processes. Increasing N and P deposition is an important driver of ecosystem changes. However, in contrast to numerous studies about the impacts of nutrient addition on forests and temperate grasslands, how plant foliar stoichiometry and nutrient resorption respond to N and P addition in alpine grasslands is poorly understood. Therefore, we conducted an N and P addition experiment (involving control, N addition, P addition, and N+P addition) in an alpine grassland on Kunlun Mountains (Xinjiang Uygur Autonomous Region, China) in 2016 and 2017 to investigate the changes in leaf nutrient concentrations (i.e., leaf N, Leaf P, and leaf N:P ratio) and nutrient resorption efficiency of Seriphidium rhodanthum and Stipa capillata, which are dominant species in this grassland. Results showed that N addition has significant effects on soil inorganic N (NO 3 --N and NH 4 + -N) and leaf N of both species in the study periods. Compared with green leaves, leaf nutrient concentrations and nutrient resorption efficiency in senesced leaves of S. rhodanthum was more sensitive to N addition, whereas N addition influenced leaf N and leaf N:P ratio in green and senesced leaves of S. capillata. N addition did not influence N resorption efficiency of the two species. P addition and N+P addition significantly improved leaf P and had a negative effect on P resorption efficiency of the two species in the study period. These influences on plants can be explained by increasing P availability. The present results illustrated that the two species are more sensitive to P addition than N addition, which implies that P is the major limiting factor in the studied alpine grassland ecosystem. In addition, an interactive effect of N+P addition was only discernable with respect to soil availability, but did not affect plants. Therefore, exploring how nutrient characteristics and resorption response to N and P addition in the alpine grassland is important to understand nutrient use strategy of plants in terrestrial ecosystems.

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Biocontrol Screening of Endophytes: Applications and Limitations

Kashyap

Singh

Yadav

et al. 2023

Plants

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The considerable loss of crop productivity each year due to plant disease or pathogen invasion during pre- or post-harvest storage conditions is one of the most severe challenges to achieving the goals of food security for the rising global population. Although chemical pesticides severally affect the food quality and health of consumers, a large population relies on them for plant disease management. But currently, endophytes have been considered one of the most suitable biocontrol agents due to better colonization and acclimatization potential. However, a very limited number of endophytes have been used commercially as biocontrol agents. Isolation of endophytes and their screening to represent potential characteristics as biocontrol agents are considered challenging by different procedures. Through a web search using the keywords “endophytes as biocontrol agents” or “biocontrol mechanism of endophytes,” we have succinctly summarised the isolation strategies and different in vitro and in vivo biocontrol screening methods of endophytic biocontrol agents in the present review. In this paper, biocontrol mechanisms of endophytes and their potential application in plant disease management have also been discussed. Furthermore, the registration and regulatory mechanism of the endophytic biocontrol agents are also covered.

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Response of fungal endophyte communities within Andropogon gerardii (Big bluestem) to nutrient addition and herbivore exclusion

Cited by 3 publications

References 90 publications

Cryptic functional diversity within a grass mycobiome

Cryptic functional diversity within a grass mycobiome

Effect of nitrogen and phosphorus addition on leaf nutrient concentrations and nutrient resorption efficiency of two dominant alpine grass species

Biocontrol Screening of Endophytes: Applications and Limitations

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