Response of heavy metal and antibiotic resistance genes and related microorganisms to different heavy metals in activated sludge

Sun, Fu-Lin; Xu, Ziqian; Fan, Leilei

doi:10.1016/j.jenvman.2021.113754

Cited by 65 publications

(22 citation statements)

References 44 publications

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“…Under the joint analysis of correlation analysis and RDA, the results showed that Cu, Zn, intI1, TP and MLs affected the absolute abundance of ARGs in different habitats of Baiyangdian Lake. This result is basically consistent with previous studies, which is showed that HMs promote the transfer of ARGs in the environment through the formation of reactive oxygen species, the increase of membrane permeability, the change of SOS response and the expression of conjugation related genes (Sun et al, 2021;Mckinney et al, 2010).…”

Section: Factors In the Arg Distributionsupporting

confidence: 93%

Occurrence and influencing factors of antibiotics and antibiotic resistance genes in sediments of the largest multi-habitat lakes in Northern China

Wang

Zhang

Liao

et al. 2022

Environ Geochem Health

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Baiyangdian Lake is a typical and largest multi-habitat lake in the North plain of China. To understand the generation and transmission of antibiotics resistance genes (ARGs) in multi-habitat lakes, the contents of nutrients (TC, TOC, TN, TP and TS), heavy metals (Zn, Cr, Ni, Cu, Pb, As, Cd and Hg), 22 antibiotics, 16S-rRNA(16S), Class I integron (intI1) and 20 ARGs were determined. Samples were taken from the Fuhe river, river estuaries, reed marshes, living area, sh poods and open water of Baiyangdian Lake. The results showed that quinolones (QNs) were the main pollutants, and the content range was ND-104.94 ng/g. Thereinto, aac (6') -IB, blaTEM-1, ermF, qnrA, qnrD, tetG, sul1, sul2 and tetM were detected in 100%. The absolute abundance of sul1 was the highest (5.25×10 5 copies/g-6.21×10 7 copies/g), which was the dominant ARGs. In these different habitats, the abundance of antibiotics and ARGs in river estuary was the highest, and that in reed marshes was the lowest. There was a signi cant positive correlation between the abundance of heavy metals (Cu, Pb, Zn, Ni, Cd, Hg) and the absolute abundance of 11 ARGs (P<0.01). Redundancy analysis showed that Cu, Zn, intI1, TP and macrolides (MLs) were the important factors affecting the distribution of ARGs. Our ndings provides a more likely driving and in uencing factor for the transmission of ARGs in lakes with complex and diverse habitats.

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Section: Factors In the Arg Distributionsupporting

confidence: 93%

Occurrence and influencing factors of antibiotics and antibiotic resistance genes in sediments of the largest multi-habitat lakes in Northern China

Wang

Zhang

Liao

et al. 2022

Environ Geochem Health

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“…Several studies have indicated that metallic elements could be also an important driver to select antibiotic resistance (Pal et al 2017;Chen et al 2019a, b;Dickinson et al 2019). Sun et al (2021) observed synergistic relationships between the majority of studied MRGs and ARGs in activated sludge. Chen et al (2015) found that exposure to extremely low concentrations of zinc, copper and arsenate induced the resistance of bacterium LSJC7 towards tetracycline.…”

Section: Co-occurrence Of Antibiotic and Metal Resistancementioning

confidence: 98%

“…Thus, the co-selection of bacterial strains or mobile genetic elements that they carry is mainly caused by co-or cross-resistance mechanisms (Pal et al 2017). The co-resistance occurs when two resistance genes are physically co-located in the same elements of a cell, such as a chromosome, a plasmid or a transposon (Sun et al 2021). In contrast, cross-resistance occurs when a single mechanism (efflux pump) provides resistance to different compounds simultaneously (Chapman 2003;Pal et al 2017).…”

Section: Co-occurrence Of Antibiotic and Metal Resistancementioning

confidence: 99%

Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditions

Mokni‐Tlili

Hechmi

Ouzari

et al. 2022

Environ Sci Pollut Res

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Urban sewage sludge (USS) is increasingly being used as an alternative organic amendment in agriculture. Because USS originates mostly from human excreta, partially metabolized pharmaceuticals have also been considered in risk assessment studies after reuse. In this regard, we investigated the cumulative effect of five annual USS applications on the spread of antibiotic-resistant bacteria (ARB) and their subsequent resistance to toxic metals in two unvegetated soils. Eventually, USS contained bacterial strains resistant to all addressed antibiotics with indices of resistance varying between 0.25 for gentamicin to 38% for ampicillin and azithromycin. Sludge-amended soils showed also the emergence of resistome for all tested antibiotics compared to non-treated controls. In this regard, the increase of sludge dose generally correlated with ARB counts, while soil texture had no influence. On the other hand, the multi-antibiotic resistance (MAR) of 52 isolates selected from USS and different soil treatments was investigated for 10 most prescribed antibiotics. Nine isolates showed significant MAR index (≥ 0.3) and co-resistance to Cd, As and Be as well. However, events including an extreme flash flood and the termination of USS applications significantly disrupted ARB communities in all soil treatments. In any case, this study highlighted the risks of ARB spread in sludge-amended soils and a greater concern with the recent exacerbation of antibiotic overuse following COVID-19 outbreak.

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“…Antibiotics could induce ARGs by a selection or co-selection mechanism [ 12 ]. Heavy metals could drive the propagation of ARGs through a co-selection mechanism [ 13 , 14 ]. Moreover, ionic liquid, polycyclic aromatic hydrocarbons (PAHs), pesticides, chlorination, and even microplastics have been proven to contribute to the dissemination of ARGs in the environment [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

The Sources and Potential Hosts Identification of Antibiotic Resistance Genes in the Yellow River, Revealed by Metagenomic Analysis

Zhang

Liu

et al. 2022

IJERPH

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The fate of antibiotic resistance genes (ARGs) has been revealed in various environmental media in recent years. Namely, the emergence of genes that resist colistin and carbapenems has attracted wide attention. However, the pollution condition of ARGs and sources in the Yellow River is still little understood, despite the river being the second longest in China. The present study determined the levels of ARG pollution in the Henan section of the Yellow River and evaluated the role of the aquaculture industry in the spread of ARGs. As revealed by the results, a total of 9 types of ARGs were detected in the sediments of the Yellow River, and the total ARG content in the Yellow River ranges from 7.27 to 245.45 RPKM. Sul1 and sul2 are the dominant ARGs, and the huge usage of sulfonamides, horizontal gene transfer, and wide bacteria host contribute to the prevalence of these two genes. The results of Spearman correlation analysis indicate that the breeding industry has little influence on ARGs in the Yellow River. Network analysis reveals that the opportunistic pathogen Pseudomonas is the potential host of sul1, tetG, and ANT(3′′)-IIa, which can pose a risk to human health.

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Response of heavy metal and antibiotic resistance genes and related microorganisms to different heavy metals in activated sludge

Cited by 65 publications

References 44 publications

Occurrence and influencing factors of antibiotics and antibiotic resistance genes in sediments of the largest multi-habitat lakes in Northern China

Occurrence and influencing factors of antibiotics and antibiotic resistance genes in sediments of the largest multi-habitat lakes in Northern China

Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditions

The Sources and Potential Hosts Identification of Antibiotic Resistance Genes in the Yellow River, Revealed by Metagenomic Analysis

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