Response of Human Blood Platelets on Nanoscale Groove Patterns: Implications for Platelet Storage

Bui, Van-Chien; Medvedev, Nikolay; Apte, Gurunath; Chen, Liyu; Denker, Christian; Greinacher, Andreas; Nguyen, Thi-Huong

doi:10.1021/acsanm.0c01326

Cited by 12 publications

(34 citation statements)

References 44 publications

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“… 4 However, this film allows inhibiting platelets only up to ∼15 min, 4 or a bit longer on laminin-coated nanopatterns. 6 With agarose, a very low density of adhered platelets was observed, while we did not observe any changes in surface morphology of platelets up to 2 h of surface contact. Our present study clearly shows a potential application of the powerful agarose gel for the inhibition of platelet-surface activation as the agarose gel is easy to fabricate without the requirement of additional materials, highly stable, and extremely inert.…”

Section: Discussioncontrasting

confidence: 64%

Controlling Surface-Induced Platelet Activation by Agarose and Gelatin-Based Hydrogel Films

et al. 2021

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Platelet-surface interaction is of paramount importance in biomedical applications as well as in vitro studies. However, controlling platelet-surface activation is challenging and still requires more effort as they activate immediately when contacting with any nonphysiological surface. As hydrogels are highly biocompatible, in this study, we developed agarose and gelatin-based hydrogel films to inhibit platelet-surface adhesion. We found promising agarose films that exhibit higher surface wettability, better controlled-swelling properties, and greater stiffness compared to gelatin, resulting in a strong reduction of platelet adhesion. Mechanical properties and surface wettability of the hydrogel films were varied by adding magnetite (Fe 3 O 4 ) nanoparticles. While all of the films prevented platelet spreading, films formed by agarose and its nanocomposite repelled platelets and inhibited platelet adhesion and activation stronger than those of gelatin. Our results showed that platelet-surface activation is modulated by controlling the properties of the films underneath platelets and that the bioinert agarose can be potentially translated to the development of platelet storage and other medical applications.

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Section: Discussioncontrasting

confidence: 64%

Controlling Surface-Induced Platelet Activation by Agarose and Gelatin-Based Hydrogel Films

et al. 2021

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“…Human blood from healthy donors who were drug-free within the previous 10 days was collected into a tube of ACD-A 1.5 mL BD-Vacutainer (Fresenius Kabi, Bad Homburg, Germany) as previously described [ 41 ]. The blood tube was sealed with parafilm and rested at room temperature for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Effect of HIT Components on the Development of Breast Cancer Cells

Chen

Apte

Lindenbauer

et al. 2021

Life

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Cancer cells circulating in blood vessels activate platelets, forming a cancer cell encircling platelet cloak which facilitates cancer metastasis. Heparin (H) is frequently used as an anticoagulant in cancer patients but up to 5% of patients have a side effect, heparin-induced thrombocytopenia (HIT) that can be life-threatening. HIT is developed due to a complex interaction among multiple components including heparin, platelet factor 4 (PF4), HIT antibodies, and platelets. However, available information regarding the effect of HIT components on cancers is limited. Here, we investigated the effect of these materials on the mechanical property of breast cancer cells using atomic force microscopy (AFM) while cell spreading was quantified by confocal laser scanning microscopy (CLSM), and cell proliferation rate was determined. Over time, we found a clear effect of each component on cell elasticity and cell spreading. In the absence of platelets, HIT antibodies inhibited cell proliferation but they promoted cell proliferation in the presence of platelets. Our results indicate that HIT complexes influenced the development of breast cancer cells.

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“…High anisotropy could guide cell contacts, this could lead to the activation process of platelet [ 59 ], activated platelets then attracts more adhesion of platelets. Another research from Bui et al [ 60 ] investigated the platelet adhesion and activation on grooved surface with 100 nm and 500 nm groove width. Results showed that 100 nm sample got a lower platelet adhesion and activation, which seems to conflict findings from Ding et al [ 57 ].…”

Section: Effects Of Topography and Surface Chemistry On Stentsmentioning

confidence: 99%

“…Secondly, 100 nm sample has much lower groove depth than 500 nm samples, this also reduce the guidance of groove structure. As a results of increase ligand density and decreased groove depth, the platelet adhesion and activation on 100 nm sample is lower than 500 nm sample [ 60 ].…”

Section: Effects Of Topography and Surface Chemistry On Stentsmentioning

confidence: 99%

Surface engineering and the application of laser-based processes to stents - A review of the latest development

Dong

Pacella

Liu

et al. 2022

Bioactive Materials

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Late in-stent thrombus and restenosis still represent two major challenges in stents’ design. Surface treatment of stent is attracting attention due to the increasing importance of stenting intervention for coronary artery diseases. Several surface engineering techniques have been utilised to improve the biological response in vivo on a wide range of biomedical devices. As a tailorable, precise, and ultra-fast process, laser surface engineering offers the potential to treat stent materials and fabricate various 3D textures, including grooves, pillars, nanowires, porous and freeform structures, while also modifying surface chemistry through nitridation, oxidation and coatings. Laser-based processes can reduce the biodegradable materials' degradation rate, offering many advantages to improve stents’ performance, such as increased endothelialisation rate, prohibition of SMC proliferation, reduced platelet adhesion and controlled corrosion and degradation. Nowadays, adequate research has been conducted on laser surface texturing and surface chemistry modification. Laser texturing on commercial stents has been also investigated and a promotion of performance of laser-textured stents has been proved. In this critical review, the influence of surface texture and surface chemistry on stents performance is firstly reviewed to understand the surface characteristics of stents required to facilitate cellular response. This is followed by the explicit illustration of laser surface engineering of stents and/or related materials. Laser induced periodic surface structure (LIPSS) on stent materials is then explored, and finally the application of laser surface modification techniques on latest generation of stent devices is highlighted to provide future trends and research direction on laser surface engineering of stents.

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Response of Human Blood Platelets on Nanoscale Groove Patterns: Implications for Platelet Storage

Cited by 12 publications

References 44 publications

Controlling Surface-Induced Platelet Activation by Agarose and Gelatin-Based Hydrogel Films

Controlling Surface-Induced Platelet Activation by Agarose and Gelatin-Based Hydrogel Films

Effect of HIT Components on the Development of Breast Cancer Cells

Surface engineering and the application of laser-based processes to stents - A review of the latest development

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