Response of Human Pulpal Tissue to Orthodontic Extrusive Applications

Sübay, Rüstem Kemal; Kaya, H; Tarım, Berna; Sübay, Arzu; Cox, Charles F.

doi:10.1097/00004770-200108000-00003

Cited by 45 publications

(34 citation statements)

References 10 publications

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“…34 Previous studies demonstrated the influence of mechanical stress in the differentiation of DPSCs, although the results are controversial. [18][19][20] Han et al revealed that continuous cyclic mechanical tension for 4-10 days increased proliferation and collagen/osteopontin expression in DPSCs. 35 Yu et al showed DPSCs increased odontogenic differentiation, early in vitro mineralization, with slightly disrupted survival by hydrostatic pressure for 2 h. 15 Cai et al indicated that a 6-h uni-axially cyclic tensile stretch inhibited osteogenic differentiation of DPSCs.…”

Section: Stretch Increases Proliferation Without Differentiation In Dmentioning

confidence: 99%

“…Some demonstrated the promotion of odontoblastic differentiation by mechanical stress in human DPSCs, 18,19 whereas others reported that mechanical stress had no significant effect on the differentiation of human dental pulp. 20 These results suggest that the response to mechanical stress differs by the type and the duration of mechanical force. Furthermore, the intracellular signaling by mechanical force was poorly investigated in DPSCs.…”

mentioning

confidence: 91%

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Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Hara

Naruse

Ozawa

et al. 2013

Tissue Engineering Part A

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Dental pulp stem cells (DPSCs), which can differentiate into several types of cells, are subjected to mechanical stress by jaw movement and occlusal forces. In this study, we evaluated how the uniaxial mechanical stretch influences proliferation and differentiation of DPSCs. DPSCs were isolated and cultured from male SpragueDawley rats. Cultured DPSCs were identified by surface markers and the differentiation capabilities as adipocytes or osteoblasts. To examine the response to mechanical stress, uniaxial stretch was exposed to cultured DPSCs. We evaluated the impact of stretch on the intracellular signaling, proliferation, osteogenic differentiation, and gene expressions of DPSCs. Stretch increased the phosphorylation of Akt, ERK1/2, and p38 MAP kinase as well as the proliferation of DPSCs. The stretch-induced proliferation of DPSCs was abolished by the inhibition of the ERK pathway. On the other hand, stretch significantly decreased the osteogenic differentiation of DPSCs, but did not affect the adipogenic differentiation. We also confirmed mRNA expressions of osteocalcin and osteopontin were significantly suppressed by stretch. In conclusion, uniaxial stretch increased the proliferation of DPSCs, while suppressing osteogenic differentiation. These results suggest a crucial role of mechanical stretch in the preservation of DPSCs in dentin. Furthermore, mechanical stretch may be a useful tool for increasing the quantity of DPSCs in vitro for regenerative medicine.

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Section: Stretch Increases Proliferation Without Differentiation In Dmentioning

confidence: 99%

mentioning

confidence: 91%

Mechanical Stretch Increases the Proliferation While Inhibiting the Osteogenic Differentiation in Dental Pulp Stem Cells

Hara

Naruse

Ozawa

et al. 2013

Tissue Engineering Part A

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“…This finding significantly expanded our expectations to explain the formation of dental pulp calcification, of which the etiology until now was ambiguous. [19][20][21][22] As far as we know today, pulp calcification is a type of chronic regressive degeneration of the pulp tissue; however, what leads to this devolution remains unclear. Dystrophic calcification is found to be of a variable degree, and this might originate from the impairment of the host cells.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the interaction between calcifying nanoparticles and human dental pulp cells: a preliminary investigation

Yang

Zeng²,

Zhang³

et al. 2010

IJN

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Calcifying nanoparticles (CNPs, previously called nanobacteria) are self-propagating, cultivable macromolecular complexes. Their extraordinary characteristic is that they can aggregate carbonate apatite on their envelope from soluble calcium and phosphorus at physiologic concentrations and display cytotoxic effects on murine and human fibroblast cell lines. The question arises whether CNPs contribute to the degeneration of pulp tissue and thus result in clinically significant human dental pulp stones as nidies. This study evaluates CNPs’ effects upon human dental pulp cells (HDPCs, the host cells in pulp tissue). We observed the ultrastructural variation of HDPCs attacked by CNPs. The spatial relationship of HDPCs and CNPs after coculture was also identified by immunofluroscence staining. Furthermore, it was verified by MTT viability assay that CNPs isolated from dental pulp stones exerted cytotoxic effect on HDPCs. Therefore, it could be concluded that the existence of CNPs might interfere with the normal physiologic function of the cells, and that might lead to dental pulp calcification. Elucidation of the cytotoxic characteristics of CNPs may offer a new perspective for understanding the etiology of human dental pulp stones.

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“…Most of researchers 1,3,7,9 stated that orthodontic force presents a negative impact on pulp tissue, whereas other researchers said that orthodontic force creates no specific impact on pulp tissue. 4,10,19 The result research on pulp tissue reaction towards orthodontic force, found histological tissue changes on almost all observed cases. The most frequent changes discovered were damages on odontoblast layer.…”

Section: Discussionmentioning

confidence: 92%

“…disrupted blood circulation, and necrosis. 4 On the contrary, other researchers testified that orthodontic forces had no prolonged significant negative effect on pulp. 3 Numerous researches on pulp tissue changes caused by orthodontic forces have been performed, among others are: pulp tissue respiration rate, 1 pulp angiogenesis, 2 pulp tissue response due to dental extrusion, 4 aspartate aminotransferase 3,5 and alkaline phosphatase 6,7 enzyme activities and pulp micro vascular response.…”

Section: Introductionmentioning

confidence: 98%