Response of<i>Callosobruchus maculatus</i>(F.) to varying temperature and relative humidity under laboratory conditions

Akpassam, Sylvia Bassey Umoetok; Iloba, B. N.; Udo, Idorenyin Asukwo

doi:10.1080/03235408.2016.1248034

Cited by 8 publications

(1 citation statement)

References 18 publications

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“…Notable exceptions include a study of temperature-dependent development in Callosobruchus rhodesianus (Pic) with the aid of X-ray photography [ 25 ] and research on Bruchus pisorum (L.) where careful examination of eggs [ 58 ] and destructive sampling of postembryonic stages [ 59 ] provide a substantially better understanding of the effects of temperature during ontogeny. By far the greatest number of articles are devoted to the temperature-dependent development in the cowpea seed beetle Callosobruchus maculatus (F.), either exclusively or alongside other Bruchinae and using various legumes as hosts [ 8 , 9 , 18 , 19 , 25 , 36 , 42 , 47 , 62 , 66 , 67 ]. All these studies but three [ 9 , 19 , 47 ], to which I will return below, only report oviposition-to-adult-emergence periods.…”

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confidence: 99%

Stagewise resolution of temperature-dependent embryonic and postembryonic development in the cowpea seed beetle Callosobruchus maculatus (F.)

Kutcherov

2020

BMC Ecol

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Background The thermal plasticity of life-history traits receives wide attention in the recent biological literature. Of all the temperature-dependent traits studied, developmental rates of ectotherms are especially often addressed, and yet surprisingly little is known about embryonic responses to temperature, including changes in the thermal thresholds and thermal sensitivity during early development. Even postembryonic development of many cryptically living species is understood superficially at best. Results This study is the first to estimate the exact durations of developmental stages in the cowpea seed beetle C. maculatus from oviposition to adult emergence at five permissive constant temperatures from 20 to 32 °C. Early embryonic development was tracked and documented by means of destructive sampling and subsequent confocal imaging of fluorescently stained specimens. Late embryonic and early larval development was studied with the use of destructive sampling and light microscopy. Well-resolved temporal series based on thousands of embryos allowed precise timing of the following developmental events: formation of the blastoderm; formation, elongation, and retraction of the germ band; dorsal closure; the onset and completion of sclerotization of the cuticle; hatching, and penetration of the first-instar larva into the cowpea seed. Pupation and adult eclosion were observed directly through an incision in the seed coat. The thermal phenotype of C. maculatus was found to vary in the course of ontogeny and different stages scaled disproportionately with temperature, but pitfalls and caveats associated with analyses of relative durations of individual stages are also briefly discussed. Conclusion Disproportionate changes in developmental durations with temperature may have important implications when study design requires a high degree of synchronization among experimental embryos or when the occurrence of particular stages in the field is of interest, as well as in any other cases when development times need to be estimated with precision. This work provides one of the first examples of integration of embryological techniques with ecophysiological concepts and will hopefully motivate similar projects in the future. While experiments with Drosophila continue to be the main source of information on animal development, knowledge on other model species is instrumental to building a broader picture of developmental phenomena.

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