Response of <i>Escherichia Coli</i> Containing Mycobacterial Carotene Genes to UV Radiation

Houssaini-Iraqui, Mohamed; Khamlichi, Naima; Yamani, Jamal El; Rastogi, Nalin

doi:10.1155/s1110724301000225

Cited by 3 publications

(8 citation statements)

References 30 publications

(59 reference statements)

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“…Airborne fungal propagules are highly adapted for survival because air is their natural dispersal medium, and they have various protection mechanisms. Apart from the capacity for repair mechanisms (Battista, 1997;Wynn-Williams et al, 2002), tolerance to UV radiation may be related to pigmentation, which for microorganisms is believed to play a protective function against injurious solar radiation (Zhdanova and Vasilevskaja, 1988;Ignoffo and Garcia, 1992;Houssaini-Iraqui et al, 2001). The spatial distribution of pigments and highly efficient DNA-repair mechanisms has a cumulative effect of ecological significance.…”

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confidence: 99%

Field study on changes in viability of airborne fungal propagules exposed to UV radiation

Ulevičius¹,

Pečiulytė

Lugauskas

et al. 2004

Environmental Toxicology

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The responses of airborne fungi to UV-B under natural conditions were investigated at the coastal station in Preila, Lithuania. Results of this investigation demonstrated that solar radiation has a marked lethal effect on outdoor airborne fungi. Sensitivity to solar radiation was the highest for the fungal propagules collected late in the evening (relative recovery 6.2%) and early morning (25.3%). The lowest sensitivity to solar radiation was observed for fungal propagules collected at midday (50.0%) and in the afternoon (53.0%). The reason for the lethal effect is thought to be elimination of the sensitive fraction of the night-time fungal populations as solar radiation gradually increases beginning at dawn. Among 356 fungus strains isolated during the investigations, 128 can be characterized as potential pathogens, and 21 strains among those most common in fungal populations belonging to plant, animal, and human pathogen groups. The collected fungal populations were exposed to solar UV-B irradiation for 2 h (from 12 p.m. to 2 p.m.). Fungal communities in the air samples were composed of saprotrophs, some of which are regarded as potential phytopathogens (Alternaria, Cladosporium, and Fusarium) or as entomopathogens (Beauveria, Paecilomyces, and Metarhizium). The airborne fungal species identified after exposure to solar radiation were predominantly: Aspergillus niger, Alternaria alternata, Cladosporium cladosporioides, Arthrinium phaerosporum, and dematiaceous sterile mycelium.

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mentioning

confidence: 99%

Field study on changes in viability of airborne fungal propagules exposed to UV radiation

Ulevičius¹,

Pečiulytė

Lugauskas

et al. 2004

Environmental Toxicology

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“…In the present study, M. smegmatis (a bacterium capable of forming non-pigmented colonies) was transformed with the pC51, vector constituted of the pHLD69 plasmid, and an insert of 4.42 kb containing genes necessary for lycopene biosynthesis [22]. All transformants obtained were orange ( fig.…”

Section: Obtaining Of Orange-pigmented Colonies Of M Smegmatismentioning

confidence: 97%

“…smegmatis MC2-155 strain was used as a host for the plasmids (pC51 and pHLD69). The pC51 plasmid consists of pHLD69 vector and an insert of approximately 4.42 kb containing the M. aurum crtE, crtB and crtI genes necessary for lycopene biosynthesis [22]. The pHLD69 vector contains pUC19, the Ori R of pAL5000, and the Kan r gene.…”

Section: Bacterial Strains Plasmids Media and Growth Conditionsmentioning

confidence: 99%

“…The mixtures were vigorously vortexed, and the pigments were then allowed to partition. The upper phase was collected, evaporated to dryness and the residue obtained was dissolved in acetone for chromatographic analysis [22]. The entire extraction process was performed under low-light conditions to prevent photo bleaching and degradation.…”

Section: Extraction and Purification Of Carotenesmentioning

confidence: 99%

“…The individual zone on the chromatogram was separated, eluted with acetone, and then, spectra were measured. Identification of carotenoids was carried out by comparing the individual characteristic absorption spectrum and chromatographic properties with a known standard and published data [22,23].…”

Section: Thin-layer Chromatography (Tlc)mentioning

confidence: 99%

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Lycopene Production in Mycobacterium Smegmatis by Expression of CRT Genes From Mycobacterium Aurum and Protective Effect of Lycopene in Vivo and in Vitro Against Uv Radiation

Elamin

Chraibi

Koraichi

et al. 2018

Int J Pharm Pharm Sci

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Objective: The aim of the present study was to express in Mycobacterium smegmatis the clustered mycobacterial genes coding for lycopene synthesis and to investigate the protective properties of lycopene against ultraviolet (UV) irradiation.Methods: The genes, which encode the biogenesis of lycopene in Mycobacterium aurum A+, were introduced into Mycobacterium smegmatis by electroporation. The pigments produced were analyzed by thin layer chromatography, and the absorption spectra were determined. A survival test using UV irradiations was also performed.Results: The transformed Mycobacterium smegmatis were found to synthesize lycopene with important yield (1.41± 3.09 mg/g) and was more resistant to ultraviolet irradiation than non-pigmented strain (p<0.01). Furthermore, cells of M. smegmatis not transformed but coated with lycopene are more resistant to UV than those uncoated (p<0.01).Conclusion: M. smegmatis can form orange colonies on agar plates when it is transformed with the lycopene genes, and the transformants produces 1.41 mg/g (dry weight) of this carotene. Our findings strongly suggest that lycopene has antioxidant activities and prevent the lethal action of UV irradiation on bacterial cells in vivo and in vitro, and deserves further studies considering the amelioration of the production.

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Protoplast fusion between Blakeslea trispora 14,271 (+) and 14,272 (−) enhanced the yield of lycopene and β-carotene

Wang

Chen

et al. 2021

World J Microbiol Biotechnol

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Response of Escherichia Coli Containing Mycobacterial Carotene Genes to UV Radiation

Cited by 3 publications

References 30 publications

Field study on changes in viability of airborne fungal propagules exposed to UV radiation

Field study on changes in viability of airborne fungal propagules exposed to UV radiation

Lycopene Production in Mycobacterium Smegmatis by Expression of CRT Genes From Mycobacterium Aurum and Protective Effect of Lycopene in Vivo and in Vitro Against Uv Radiation

Protoplast fusion between Blakeslea trispora 14,271 (+) and 14,272 (−) enhanced the yield of lycopene and β-carotene

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