Response of Ig-positive cells to Enteromyxum scophthalmi (Myxozoa) experimental infection in turbot, Scophthalmus maximus (L.): A histopathological and immunohistochemical study

Bermúdez, Roberto; Vigliano, Fabricio Andrés; Marcaccini, A.; Sitjà‐Bobadilla, Ariadna; Quiroga, María Isabel; Nieto, J. M.

doi:10.1016/j.fsi.2006.02.006

Cited by 46 publications

(73 citation statements)

References 36 publications

(37 reference statements)

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“…This observation supports the hypothesis that the MMCs may be involved in fish immune responses during infections (Bermúdez et al 2006b, Vigliano et al 2006.…”

Section: Discussionsupporting

confidence: 90%

“…Scale bar = 2 µm sponse vary greatly depending on the host species (Padrós et al 2001, Álvarez-Pellitero et al 2008, Fleurance et al 2008. Similarly, the current results on turbot enteromyxosis demonstrated that the inflammatory response in the digestive tract was not always correlated with the number of parasites populating the epithelium, as has been previously reported (Bermúdez et al 2006b, 2007). An interesting finding was the initial increase in the amount of both rodlet and goblet cells, followed by a decrease in their number in fish suffering severe infections (Vigliano et al 2004).…”

Section: Discussionsupporting

confidence: 84%

“…In addition, the lymphohaematopoietic depletion in the spleen and kidney of Enteromyxum scophthalmi-infected turbot described by was confirmed by the current observations. Determining whether this finding is due to the recruitment and migration of lymphocytes to the digestive tract (Bermúdez et al 2006b) or to a parasite-induced apoptosis in the immune cells from these organs deserves further investigation.…”

Section: Discussionmentioning

confidence: 99%

“…Studies concerning Enteromyxum scophthalmi infections in turbot have been focused on the epidemiology and immunopathology of the disease (Palenzuela et al 2002, Redondo et al 2004, Bermúdez et al 2006b, 2008 rather than on the lesions that it induces in host tissues. Therefore, scarce data exist on the histological changes that enteromyxosis causes in turbot.…”

Section: Introductionmentioning

confidence: 99%

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Light and electron microscopic studies on turbot Psetta maxima infected with Enteromyxum scophthalmi: histopathology of turbot enteromyxosis

Bermúdez¹,

Losada²,

Vázquez³

et al. 2010

Dis. Aquat. Org.

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In the last decade, a new parasite that causes severe losses has been detected in farmed turbot Psetta maxima (L.), in north-western Spain. The parasite was classified as a myxosporean and named Enteromyxum scophthalmi. The aim of this study was to characterize the main histological changes that occur in E. scophthalmi-infected turbot. The parasite provoked catarrhal enteritis, and the intensity of the lesions was correlated with the progression of the infection and with the development of the parasite. Infected fish were classified into 3 groups, according to the lesional degree they showed (slight, moderate and severe infections). In fish with slight infections, early parasitic stages were observed populating the epithelial lining of the digestive tract, without eliciting an evident host response. As the disease progressed, catarrhal enteritis was observed, the digestive epithelium showed a typical scalloped shape and the number of both goblet and rodlet cells was increased. Fish with severe infections suffered desquamation of the epithelium, with the subsequent release of parasitic forms to the lumen. The dislodged enterocytes underwent anoikis, a mode of apoptosis triggered by the loss of anchorage, which might facilitate spreading of the parasite. Lymphohaematopoietic depletion was also observed, mainly in head kidney and spleen, which could contribute to the high virulence of this parasite.

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“…This observation supports the hypothesis that the MMCs may be involved in fish immune responses during infections (Bermúdez et al 2006b, Vigliano et al 2006.…”

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Light and electron microscopic studies on turbot Psetta maxima infected with Enteromyxum scophthalmi: histopathology of turbot enteromyxosis

Bermúdez¹,

Losada²,

Vázquez³

et al. 2010

Dis. Aquat. Org.

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“…Although in the previous report of Fournier-Betz et al (2000) as in our study the fish were not experimentally exposed to antigens, the differences observed in Ig+ cell distribution may be related to the previous immunological history of each group. As was previously stated in other haemolymphopoietic organs of turbot (Bermúdez et al 2006), no clear association within Ig+ cells and melano-macrophage centres was observed in the thymus of healthy turbot.…”

Section: Discussionsupporting

confidence: 60%

Morphological and immunohistochemical characterisation of the thymus in juvenile turbot (Psetta maxima, L.)

Vigliano

Losada

Castello³

et al. 2011

Cell Tissue Res

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We performed structural and immunohistochemical studies on the thymus of juvenile turbot (Psetta maxima L.) in order to define its cellular composition. The thymic stroma was mainly composed of two subpopulations of reticulo-epithelial cells (RECs). RECs immunoreactive to anti-actin antibody were distributed through the organ, while RECs that were cytokeratin-immunopositive were located in the outer zone of the thymus. The parenchyma of the thymus was composed of several cell types such as lymphocytes/thymocytes, lymphoblasts, melano-macrophages and to a lesser extent of nurse-like cells, immunoglobulin positive (Ig+) cells, mucous cells, rodlet cells and neuroendocrine cells. CD3ε+ lymphocytes were mainly located in the outer zone. On the other hand, Ig+ cells were observed in the transitional region between the inner and outer zones of the thymus. The neuroendocrine cells were large and exhibited immunoreactivity to neuropeptide Y and vasoactive intestinal polypeptide. They were located in the inner zone of the thymus in close association with lymphoblasts and lymphocytes/thymocytes. This work provides useful information on the structure and cellular composition of the thymus of turbot, identifying several immunomarkers that allow the identification of different cell types, providing the basis for further studies on the immune response of turbot against diseases.

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Fish Immune Responses to Myxozoa

Sitjà‐Bobadilla

Schmidt‐Posthaus

Wahli

et al. 2015

Myxozoan Evolution, Ecology and Development

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Response of Ig-positive cells to Enteromyxum scophthalmi (Myxozoa) experimental infection in turbot, Scophthalmus maximus (L.): A histopathological and immunohistochemical study

Cited by 46 publications

References 36 publications

Light and electron microscopic studies on turbot Psetta maxima infected with Enteromyxum scophthalmi: histopathology of turbot enteromyxosis

Light and electron microscopic studies on turbot Psetta maxima infected with Enteromyxum scophthalmi: histopathology of turbot enteromyxosis

Morphological and immunohistochemical characterisation of the thymus in juvenile turbot (Psetta maxima, L.)

Fish Immune Responses to Myxozoa

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