Response of in vitro reared honey bee larvae to various doses of Paenibacillus larvae larvae spores

Brødsgaard, Camilla J.; Ritter, W.; Hansen, Hans Peter

doi:10.1051/apido:19980609

Cited by 94 publications

(78 citation statements)

References 12 publications

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“…They infect the larvae while feeding them food contaminated with the infectious spores. The younger the larvae, the more susceptible they are to infection (RiessbergerGalle et al 2001;Brødsgaard et al 1998). The spores germinate in the larval midgut, approximately one day after ingestion.…”

Section: Biology/pathogenesis/epidemiologymentioning

confidence: 99%

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The Honeybee Disease American Foulbrood — An African Perspective

et al. 2011

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Sustaining apiculture worldwide has been threatened by bee diseases and unexplained colony losses. African honeybee populations seem healthier and no major losses have been reported despite the presence of all the major pests and diseases. The scattered colonies in the large wild population of the continent might ensure slow pathogen spread and thus protect the unmanaged colonies in comparison with the concentration of colonies in managed apiaries. Beekeeping and trade in bee products is responsible for spreading many diseases throughout the world. The recent outbreak of the bacterial disease American foulbrood (AFB) in South Africa is a matter of great concern. Despite a growing number of apiaries testing positive for AFB, no major colony losses have been reported yet. This could be based on higher disease resistance of African honeybees, but such a trait might not persist if pathogens accumulate in the hives. In the first part of this article we review what is known on the history, biology and epidemiology of AFB as well as the control methods available. We then argue that given the particular context of honeybees in Africa, protection policies need to be put in place to ensure that African honeybees remain healthy.

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Section: Biology/pathogenesis/epidemiologymentioning

confidence: 99%

“…AFB is one of the most severe bee diseases (Riessberger-Galle et al 2001;Brødsgaard et al 1998). It is classified as a List B disease by the OIE (Office International des Epizooties / World Organization for Animal Health) and is considered a transmissible disease of socio-economic importance (De Graaf et al 2006).…”

Section: Introductionmentioning

confidence: 99%

The Honeybee Disease American Foulbrood — An African Perspective

et al. 2011

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“…Spores are only infectious to young larvae that become infected by consuming spore-contaminated food (Hitchcock et al 1979). As few as 10 spores are enough to infect and kill a larva (Brødsgaard et al 1998;Woodrow 1942). The most conspicuous symptom in colonies suffering from clinical AFB disease is irregular brood capping, with capped and uncapped cells scattered irregularly across the brood frames.…”

Section: Introductionmentioning

confidence: 99%

Prognostic value of using bee and hive debris samples for the detection of American foulbrood disease in honey bee colonies

Forsgren

Laugen

2013

Apidologie

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-The objective of this study was to compare how well the detection of Paenibacillus larvae in samples of live bees or in accumulated winter debris collected from the bottom of beehives relates to symptoms of American foulbrood in honey bee colonies. Fifty-eight colonies in one commercial beekeeping operation were inspected for disease symptoms and assayed for P. larvae using culture-based techniques and PCR. The results show that culture-based techniques are more accurate than recently published PCR methods for detecting the bacterium in clinically diseased colonies, and that the prognostic value of bacterial colony counts from bee samples is superior to colony counts from debris. However, if the objective is to monitor the prevalence of the bacterium irrespective of disease symptoms, the preferable method is PCR analysis of accumulated winter hive debris.American foulbrood / Paenibacillus larvae / culture / PCR / logistic regression

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“…Rinderer and Rothenbuhler (1974) demonstrated the influence of pollen on the mortality of spore-fed larvae. Pollen contains microorganisms which act as antagonists of P. larvae larvae (Brødsgaard et al, 1998). The presence of pollen in the intestine of the larvae may act to inhibit P. larvae larvae (Reiche et al, 1996).…”

Section: Wedenig Et Almentioning

confidence: 99%

A substance in honey bee larvae inhibits the growth of Paenibacillus larvae larvae

Wedenig

Riessberger-Gallé

2003

Apidologie

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-Paenibacillus larvae larvae, a Gram-positive and spore-forming bacterium, is the cause of American foulbrood. We investigated the resistance of larvae of different ages from different colonies against P. larvae larvae. We prepared ethanol-water-extracts from two, three, four and five day old larvae and also larvae in two capped stages to test the ability of the homogenates to inhibit the growth of P. larvae larvae in vitro. There were age and colony dependent differences in the inhibiting potentials of larvae. The results suggest that the concentration of the inhibiting substance(s) in the extract might be responsible for the known different degree of resistance against P. larvae larvae in vivo. Our results further show that the extracts of the two-, three-and four-day-old larvae have the ability to inhibit the growth of P. larvae larvae very well, while starting around day five the inhibiting effect is gradually reduced.Apis mellifera carnica / Paenibacillus larvae larvae / honeybee larvae / age-related resistance /

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Response of in vitro reared honey bee larvae to various doses of Paenibacillus larvae larvae spores

Cited by 94 publications

References 12 publications

The Honeybee Disease American Foulbrood — An African Perspective

The Honeybee Disease American Foulbrood — An African Perspective

Prognostic value of using bee and hive debris samples for the detection of American foulbrood disease in honey bee colonies

A substance in honey bee larvae inhibits the growth of Paenibacillus larvae larvae

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