Response of larval barnacle proteome to CO2-driven seawater acidification

Wong, Kelvin K.W.; Lane, Ackley; Leung, Priscilla T.Y.; Thiyagarajan, V.

doi:10.1016/j.cbd.2011.07.001

Cited by 32 publications

(37 citation statements)

References 87 publications

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“…This is important as the non-oxidative part recycles ribulose 5-phosphate to glucose 6-phosphate, generating a cycle that leads to the conversion of glucose 6-phosphate to six CO 2 and 12 NADPH (Nelson and Cox, 2008). Transketolase was also more abundant in larvae of the barnacle Balanus amphitrite in response to CO 2 -enriched acidic ( pH 7.6) seawater (Wong et al, 2011).…”

Section: Abundance Changes Of Nadph-generating Enzymes During Environmentioning

confidence: 93%

“…In addition, the Trx homolog nucleoredoxin also increased in abundance in response to low pH, suggesting that the SOD-Trx-Prx system was activated to respond to increasing levels of ROS, either as a direct effect of pH via the Fenton reaction or indirectly by CO 2 interacting with peroxynitrite, resulting in reactive carbonate, oxygen and nitrogen species (Trujillo et al, 2008). Three other studies investigating the response to low pH in marine invertebrate larvae did not identify a single antioxidant protein among those proteins that were detected and that changed significantly with low pH (Dineshram et al, 2013(Dineshram et al, , 2012Wong et al, 2011).…”

Section: Ph-induced Antioxidant Responsementioning

confidence: 99%

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Proteomic responses to environmentally induced oxidative stress

Tomanek

2015

Journal of Experimental Biology

140

132

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Environmental (acute and chronic temperature, osmotic, hypoxic and pH) stress challenges the cellular redox balance and can lead to the increased production of reactive oxygen species (ROS). This review provides an overview of the reactions producing and scavenging ROS in the mitochondria, endoplasmic reticulum (ER) and peroxisome. It then compares these reactions with the findings of a number of studies investigating the proteomic responses of marine organisms to environmentally induced oxidative stress. These responses indicate that the thioredoxin-peroxiredoxin system is possibly more frequently recruited to scavenge H 2 O 2 than the glutathione system. Isoforms of superoxide dismutase (SOD) are not ubiquitously induced in parallel, suggesting that SOD scavenging activity is sometimes sufficient. The glutathione system plays an important role in some organisms and probably also contributes to protecting protein thiols during environmental stress. Synthesis pathways of cysteine and selenocysteine, building blocks for glutathione and glutathione peroxidase, also play an important role in scavenging ROS during stress. The increased abundance of glutaredoxin and DyP-type peroxidase suggests a need for regulating the deglutathionylation of proteins and scavenging of peroxynitrite. Reducing equivalents for these scavenging reactions are generated by proteins of the pentose phosphate pathway and by NADP-dependent isocitrate dehydrogenase. Furthermore, proteins representing reactions of the tricarboxylic acid cycle and the electron transport system generating NADH and ROS, including those of complex I, II and III, are frequently reduced in abundance with stress. Protein maturation in the ER likely represents another source of ROS during environmental stress, as indicated by simultaneous changes in ER chaperones and antioxidant proteins. Although there are still too few proteomic analyses of non-model organisms exposed to environmental stress for a general pattern to emerge, hyposaline and low pH stress show different responses from temperature and hypoxic stress. Furthermore, comparisons of closely related congeners differing in stress tolerance start to provide insights into biochemical processes contributing to adaptive differences, but more of these comparisons are needed to draw general conclusions. To fully take advantage of a systems approach, studies with longer time courses, including several tissues and more species comparisons are needed.

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Section: Abundance Changes Of Nadph-generating Enzymes During Environmentioning

confidence: 93%

Section: Ph-induced Antioxidant Responsementioning

confidence: 99%

Proteomic responses to environmentally induced oxidative stress

Tomanek

2015

Journal of Experimental Biology

140

132

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“…Recently, proteomics analyses have been recognized as promising molecular tools that can be used to study larval development (Thiyagarajan and Qian, 2008), metamorphosis (Mok et al, 2009), perception of chemical signals ) and response to various environmental changes including OA (Dineshram et al, 2012;Wong et al, 2011). Although twodimensional gel electrophoresis (2-DE)-based proteomics is widely used in larval biology research, it has been used in only a few climate change studies (Thiyagarajan, 2010;Tomanek, 2011).…”

Section: Introductionmentioning

confidence: 99%

Proteomic response of marine invertebrate larvae to ocean acidification and hypoxia during metamorphosis and calcification

Mukherjee

Wong

Chandramouli

et al. 2013

Journal of Experimental Biology

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SUMMARYCalcifying marine invertebrates with complex life cycles are particularly at risk to climate changes as they undergo an abrupt ontogenetic shift during larval metamorphosis. Although our understanding of the larval response to climate changes is rapidly advancing, the proteome plasticity involved in a compensatory response to climate change is still unknown. In this study, we investigated the proteomic response of metamorphosing larvae of the tubeworm Hydroides elegans, challenged with two climate change stressors, ocean acidification (OA; pH 7.6) and hypoxia (HYP; 2.8 mg O 2 l −1 ), and with both combined. Using a twodimensional gel electrophoresis (2-DE)-based approach coupled with mass spectrometry, we found that climate change stressors did not affect metamorphosis except under OA, but altered the larval proteome and phosphorylation status. Metabolism and various stress and calcification-related proteins were downregulated in response to OA. In OA and HYP combined, HYP restored the expression of the calcification-related proteins to the control levels. We speculate that mild HYP stress could compensate for the negative effects of OA. This study also discusses the potential functions of selected proteins that might play important roles in larval acclimation and adaption to climate change.Supplementary material available online at http://jeb.biologists.org/lookup/suppl

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“…Intertidal barnacles as conspicuous, widely distributed, and ecologically important species have served to study the eVects of environmental factors, e.g., temperature, salinity, food type and concentration, light (e.g., Crisp and Ritz 1973;Harms 1984;Holm 1990;Konya and Miki 1994;Anil et al 1995Anil et al , 2001Anil and Kurian 1996;Qian 1997, 1999;Hentschel and Emlet 2000;Thiyagarajan et al 2002a;Nasrolahi et al 2007), and more recently global warming and ocean acidiWcation (Findlay et al 2008;Hung et al 2008;Findlay et al 2009;McDonald et al 2009;Wong et al, in press) on larval development and settlement success. Much of this progress on what is frequently considered the most sensitive life history phase is facilitated because barnacle larvae can be easily reared under controlled conditions (Thiyagarajan et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Being young in a changing world: how temperature and salinity changes interactively modify the performance of larval stages of the barnacle Amphibalanus improvisus

et al. 2011

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The fate of key species, such as the barnacle Amphibalanus improvisus, in the course of global change is of particular interest since any change in their abundance and/or performance may entail community-wide eVects. In the Xuctuating Western Baltic, species typically experience a broad range of environmental conditions, which may preselect them to better cope with climate change. In this study, we examined the sensitivity of two crucial ontogenetic phases (naupliar, cypris) of the barnacle toward a range of temperature (12, 20, and 28°C) and salinity (5, 15, and 30 psu) combinations. Under all salinity treatments, nauplii developed faster at intermediate and high temperatures. Cyprid metamorphosis success, in contrast, was interactively impacted by temperature and salinity. Survival of nauplii decreased with increasing salinity under all temperature treatments. Highest settlement rates occurred at the intermediate temperature and salinity combination, i.e., 20°C and 15 psu. Settlement success of "naive" cyprids, i.e., when nauplii were raised in the absence of stress (20°C/15 psu), was less impacted by stressful temperature/ salinity combinations than that of cyprids with a stress history. Here, settlement success was highest at 30 psu particularly at low and high temperatures. Surprisingly, larval survival was not highest under the conditions typical for the Kiel Fjord at the season of peak settlement (20°C/15 psu). The proportion of nauplii that ultimately transformed to attached juveniles was, however, highest under these "home" conditions. Overall, only particularly stressful combinations of temperature and salinity substantially reduced larval performance and development. Given more time for adaptation, the relatively smooth climate shifts predicted will probably not dramatically aVect this species.

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Response of larval barnacle proteome to CO2-driven seawater acidification

Cited by 32 publications

References 87 publications

Proteomic responses to environmentally induced oxidative stress

Proteomic responses to environmentally induced oxidative stress

Proteomic response of marine invertebrate larvae to ocean acidification and hypoxia during metamorphosis and calcification

Being young in a changing world: how temperature and salinity changes interactively modify the performance of larval stages of the barnacle Amphibalanus improvisus

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