Response of light- and dark-grown callus of atrazine-resistant and susceptible rapeseed (Brassica napus) to varying concentrations of atrizine

Abstract

Response of light-and dark-grown callus of atrazine-resistant and susceptible rapeseed (Brassica napus) to varying concentrations of atrazine Abstract. Callus cultures initiated with tissue of triazine-resistant and susceptible lines of Brassica napus L. were cultured in the presence of atrazine (10 -s to 10 -4 M) under a 16-h photoperiod or in continuous darkness. On atrazine-free media callus growth was characteristically greater for the light-grown cultures. On media containing atrazine, growth of callus from triazine-resistant and susceptible rapeseed cultured in the dark did not differ significantly. Addition of atrazine to the culture medium resulted in a significant reduction in the growth of light-grown callus of the susceptible genotype to a level equivalent to that of the dark-grown cultures. Atrazine had no effect on the growth of light-grown callus initiated with triazine-resistant rapeseed.

Analysis of organelle genomes in a somatic hybrid derived from cytoplasmic male-sterile Brassica oleracea and atrazine-resistant B. campestris

“…Experiments using 4-week-old p-calli from fertile, hybrid Green Comet showed that 1-50 ~tM atrazine in medium E with 1% sucrose reduced the growth rate in the light by a factor of 4, to a rate comparable to darkgrown calli (data not shown). Grant et al (1983) also reported that atrazine reduced the growth of atrazine sensitive B. napus callus to levels of dark-grown callus and that growth of atrazine-resistant B. napus callus was not inhibited by atrazine. As the number of shoots regenerated in the fusion experiment reported here was small (four), it was not possible to determine the effectiveness ofatrazine as an in vitro selective agent.…”