Response of osteoblast‐like SAOS‐2 cells to zirconia ceramics with different surface topographies

Hempel, Ute; Hefti, Thomas; Kalbáčová, Marie Hubálek; Wolf‐Brandstetter, Cornelia; Dieter, Peter; Schlottig, Falko

doi:10.1111/j.1600-0501.2009.01797.x

Cited by 117 publications

(93 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…27 with p-nitrophenyl phosphate as a substrate. TNAP activity was calculated from a linear calibration curve (r Ͼ 0.99) prepared with p-nitrophenol.…”

Section: Methodsmentioning

confidence: 99%

“…Immunofluorescence Staining-To visualize annexin V and VI at day 22, immunofluorescence staining was performed as described before (27). Briefly, after washing, fixing, and permeabilization, hBMSC were incubated with rabbit anti-human annexin V polyclonal antibody (catalog no.…”

Section: Methodsmentioning

confidence: 99%

“…Indeed, it has been shown that synthetically sulfated GAG derivatives promote osteogenic differentiation of human mesenchymal stromal cells and influence manifold cellular functions (14,25,27). Additionally, sulfated GAGs were shown to inhibit the bone degradation by osteoclasts (72) and to provide immunomodulation properties (73).…”

Section: Comparison Of the Matrix Vesicle Proteome With Hbmsc Proteomementioning

confidence: 99%

See 2 more Smart Citations

Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans

Schmidt

Kliemt

Preissler

et al. 2016

Molecular & Cellular Proteomics

Self Cite

View full text Add to dashboard Cite

Our aging population has to deal with the increasing threat of age-related diseases that impair bone healing. One promising therapeutic approach involves the coating of implants with modified glycosaminoglycans (GAGs) that mimic the native bone environment and actively facilitate skeletogenesis. In previous studies, we reported that coatings containing GAGs, such as hyaluronic acid (HA) and its synthetically sulfated derivative (sHA1) as well as the naturally low-sulfated GAG chondroitin sulfate (CS1), reduce the activity of bone-resorbing osteoclasts, but they also induce functions of the bone-forming cells, the osteoblasts. However, it remained open whether GAGs influence the osteoblasts alone or whether they also directly affect the formation, composition, activity, and distribution of osteoblast-released matrix vesicles (MV), which are supposed to be the active machinery for bone formation. Here, we studied the molecular effects of sHA1, HA, and CS1 on MV activity and on the distribution of marker proteins. Furthermore, we used comparative proteomic methods to study the relative protein compositions of isolated MVs and MV-releasing osteoblasts. The MV proteome is much more strongly regulated by GAGs than the cellular proteome. GAGs, especially sHA1, were found to severely impact vesicle-extracellular matrix interaction and matrix vesicle activity, leading to stronger extracellular matrix formation and mineralization. This

show abstract

“…27 with p-nitrophenyl phosphate as a substrate. TNAP activity was calculated from a linear calibration curve (r Ͼ 0.99) prepared with p-nitrophenol.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Comparison Of the Matrix Vesicle Proteome With Hbmsc Proteomementioning

confidence: 99%

See 1 more Smart Citation

Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans

Schmidt

Kliemt

Preissler

et al. 2016

Molecular & Cellular Proteomics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Chemical etching has been reported to be an efficient technique to produce a fine roughness on zirconia, down to the nanoscale (Hempel et al, 2010). Gahlert et al evidenced that hydrofluoric acid (HF) etching of zirconia implants enhances bone apposition resulting in high removal torque values (Gahlert et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Surface roughened zirconia: towards hydrothermal stability

Camposilvan

Flamant

Anglada

2015

Journal of the Mechanical Behavior of Biomedical Materials

View full text Add to dashboard Cite

Surface roughness is needed in several yttria-stabilized zirconia components used in restorative dentistry for osseointegration or adhesion purposes. This can be achieved by different treatments, which may also modify the microstructure of the surface. Among them, sandblasting and chemical etching are widely used, but their effect on hydrothermal aging of zirconia is not fully understood. In the present work, the zirconia long-term stability of rough surfaces prepared by these techniques is analyzed and a method is proposed for preventing hydrothermal aging while maintaining the original surface appearance and mechanical properties. The method involves pressure infiltration of a Cerium salt solution on the roughened surfaces followed by a thermal treatment. The solution, trapped by surface defects and small pores, is decomposed during thermal treatment into Cerium oxide, which is diffused at high temperature, obtaining Ce co-doping in the near-surface region.In addition, the microstructural changes induced in the near-surface by sandblasting or chemical etching are removed by the thermal treatment together with surface defects. No color modification was observed and the final roughness parameters were in the range of existing implants of proved good osseointegration. The aging resistance of Ce co-doped materials was strongly enhanced, showing the absence of aging after artificial degradation, increasing in this way the surface mechanical integrity.The proposed treatment is easily applicable to the current manufacturing procedures of zirconia dental posts, abutments, crowns and dentures, representing a solution to hydrothermal aging in these and other biomedical applications.

show abstract

“…13,17 A lowpressure air abrasion method was proposed that reduces surface damage. 42 The alkaline phosphate activity was used to evaluate the differentiation of cells of the osteogenic lineage and compare the cell activity in different tested groups. 43 No significant difference in alkaline phosphate activity was noted between all groups, indicating that type of material and surface treatment did not affect the enzyme activity, which is in accordance with other studies.…”

Section: Discussionmentioning

confidence: 99%

Surface Characterization and Cell Adhesion of Different Zirconia Treatments: An in vitro Study

Nassif

Rifai

2018

The Journal of Contemporary Dental Practice

View full text Add to dashboard Cite

Aim:The aim of this study was to characterize the surface of zirconia subjected to different treatments and evaluate its effect on cell adhesion and proliferation. Materials and methods:A total of 80 zirconia disks were divided into four groups (n = 20) according to the surface treatments used: group I: as-sintered (AS), no surface treatment applied; group II: abrasion treatment applied using Rocatec (ROC; 3M ESPE) system with silica-coated alumina powder of grit size 110 μm; group III: erbium, chromium:yttrium, scandium, gallium, garnet (Er, Cr:YSGG) laser (LAS; BIOLASE) was used at a frequency of 20 Hz and output power of 3 W; and group IV: specimens were subjected to the selective infiltration etching (SIE) technique. Surface characterization was evaluated for the different groups (roughness, hardness, and morphology), and cell behavior (adhesion and proliferation) was tested (α = 0.05). Results:The ROC group reported a significant increase in surface roughness (2.201 ± 0.352) and Vickers hardness (1758 ± 16.6) compared with the other surface treatments. The SIE surface-treated group reported a significantly higher number of cells (64.5 ± 2.6 and 53.5 ± 2.2 respectively) compared with the other surface-treated groups. Conclusion:The SIE is a promising surface treatment for zirconia that significantly enhances cell adhesion and osseointegration.Clinical significance: The SIE treatment of zirconia implants may help in a faster and better osseointegration.

show abstract

Response of osteoblast‐like SAOS‐2 cells to zirconia ceramics with different surface topographies

Abstract: These data indicate that zirconia mediates a pronounced stronger effect on the adhesion, proliferation and differentiation compared with titanium; and that topographical differences of zirconia have minor effects on osteoblast biology.

Cited by 117 publications

References 25 publications

Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans

Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans

Surface roughened zirconia: towards hydrothermal stability

Surface Characterization and Cell Adhesion of Different Zirconia Treatments: An in vitro Study

Contact Info

Product

Resources

About