Response of Phosphoenolpyruvate Carboxylase from Maize Leaves to Moderate Water Deficit

Rodríguez-Penagos, Mireya; Muñoz‐Clares, Rosario A.

doi:10.1016/s0176-1617(99)80065-0

Cited by 5 publications

(2 citation statements)

References 43 publications

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“…Protease and phosphatases inhibitors were not added to the extraction buffer to avoid PEPC activity lost because PEPC activity in leaf extracts was measured rapidly and very diluted (See Echevarria et al ), and we performed tests and found that similar results were obtained with and without adding inhibitors (see Tables S1, S2). In addition, extracts were not desalted because our comparative essays (Table S2) and previous studies have shown similar results with and without desalting (Rodrïguez‐Penagos and Muñoz‐Clares ; Avasthi and Raghavendra ; Gandullo et al ). Anti‐body anti‐PEPC and anti‐phosphorylation site at serine close to the N‐terminal site of the enzyme were applied to confirm that during enzymatic extractions the PEPC did not lose its N‐terminal end and that crude extracts obtained rapidly in the absence of proteases inhibitors and diluted are adequate to measure PEPC activity and IC 50 values in Spartina taxa (See Fig.…”

Section: Methodsmentioning

confidence: 67%

Changes to the functional traits of phosphoenolpyruvate carboxylase following hybridization in C‐4 halophytes

Gallego‐Tévar

Peinado-Torrubia

Álvarez

et al. 2020

Physiologia Plantarum

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Hybridization is a relevant evolutionary mechanism linked to the invasiveness of plant species, but little is known about its effect on enzymatic activities in response to stress. We analyzed the effects of salinity on key mechanistic traits of phosphoenolpyruvate carboxylase (PEPC) enzyme for two hybrid taxa derived from native Spartina maritima (Curtis) Fernald and invasive Spartina densiflora Brongn. in comparison with their parental species. Parental species showed contrasted strategies at the PEPC level to cope with salinity. Spartina maritima showed its physiological optimum at 10 to 40 ppt salinity, with high PEPC activity (per unit leaf soluble protein), in contrast to the lower salinity optimum of 0.5 and 10 ppt for S. densiflora, where highest levels of PEPC apparent specific activity coincided with high light-induced activation of PEPC. Both hybrids showed constant PEPC apparent specific activity from fresh water to hypersalinity and exhibited higher net photosynthesis rates in fresh water than their parents. Spartina maritima × densiflora presented three transgressive PEPC-related traits, being the only taxon able to increase its PEPC activation in darkness at high salinity. Spartina densiflora × maritima showed most PEPC-related traits intermediate between its parents. Inheritance types operating differently in reciprocal hybrids determine key functional traits conditioning their ecological performance.

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Section: Methodsmentioning

confidence: 67%

Changes to the functional traits of phosphoenolpyruvate carboxylase following hybridization in C‐4 halophytes

Gallego‐Tévar

Peinado-Torrubia

Álvarez

et al. 2020

Physiologia Plantarum

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“…Protease and phosphatase inhibitors were not added to the extraction buffer to avoid PEPC activity lost since PEPC activity in leaf extracts was measured rapidly and very diluted (5 μl of crude extract in a final volume of 1000 μl); similar results have been obtained with and without adding inhibitors ( Echevarría et al ., 1990 ; Gallego-Tévar et al ., 2019a ). Extracts were not desalted as previous studies have shown similar results with and without desalting ( Rodríguez-Penagos and Muñoz-Clares, 1999 ; Gandullo et al ., 2019 ; Gallego-Tévar et al ., 2019a ). The total protein amount was determined following the colorimetric method of Bradford (1976 ), using bovine serum albumin as standard.…”

Section: Methodsmentioning

confidence: 99%

From physiology to salt marsh management challenges with sea level rise: the case of native Spartina foliosa, invasive S. densiflora and their hybrid

Gallego‐Tévar

Peinado-Torrubia

Álvarez

et al. 2020

Conservation Physiology

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Abstract Sea level rise (SLR) imposes increasing salinity and inundation stresses in salt marshes which simultaneously face invasions by exotic plant species. We aimed to improve and apply knowledge on the ecophysiological responses of halophytes to SLR to conservation management of salt marshes. In a mesocosm experiment, we measured and compared phosphoenolpyruvate carboxylase (PEPC) activity and related functional traits of the California-native Spartina foliosa, invasive S. densiflora and their hybrid S. densiflora × foliosa in response to increasing levels of salinity and inundation. S. foliosa was moderately sensitive to salinity, showing a 57% reduction in PEPC specific activity from freshwater to hypersalinity. This native species compensated for the reduction of PEPC activity with increased salinity through 80% higher enzyme activation by phosphorylation. PEPC functional trait responses of S. foliosa were mostly independent of inundation depth. In view of these results, managers should conserve undeveloped lands for accommodation space above current high tide lines to facilitate colonization of stress-tolerant S. foliosa. Our results on functional responses of PEPC traits recorded high sensitivity to salinity for S. densiflora. This was reflected by 65% lower PEPC specific activity together with increasing accumulation of free proline (+96%) and total proteins (+23%) with elevated salinity. These results suggest prioritized eradication of S. densiflora populations in brackish habitats. Measured PEPC responses support the high stress tolerance of the S. densiflora × foliosa hybrid. PEPC traits for the hybrid were mostly independent of salinity and inundation. The hybrid showed higher PEPC-specific activity than S. foliosa (+70%) and S. densiflora (+15%) in freshwater under intermediate inundation. Results suggest that eradication of the hybrid should be the highest management priority. Our study shows that the responses of key functional physiological traits to environmental stresses serve as biological indicators that can guide ecosystem management practices in a scenario of climate change.

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Transcriptome sequencing of the Antarctic vascular plant Deschampsia antarctica Desv. under abiotic stress

Lee

Noh

Choi³

et al. 2012

Planta

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Antarctic hairgrass (Deschampsia antarctica Desv.) is the only natural grass species in the maritime Antarctic. It has been studied as an extremophile that has successfully adapted to marginal land with the harshest environment for terrestrial plants. However, limited genetic research has focused on this species due to the lack of genomic resources. Here, we present the first de novo assembly of its transcriptome by massive parallel sequencing and its expression profile using D. antarctica grown under various stress conditions. Total sequence reads generated by pyrosequencing were assembled into 60,765 unigenes (28,177 contigs and 32,588 singletons). A total of 29,173 unique protein-coding genes were identified based on sequence similarities to known proteins. The combined results from all three stress conditions indicated differential expression of 3,110 genes. Quantitative reverse transcription polymerase chain reaction showed that several well-known stress-responsive genes encoding late embryogenesis abundant protein, dehydrin 1, and ice recrystallization inhibition protein were induced dramatically and that genes encoding U-box-domain-containing protein, electron transfer flavoprotein-ubiquinone, and F-box-containing protein were induced by abiotic stressors in a manner conserved with other plant species. We identified more than 2,000 simple sequence repeats that can be developed as functional molecular markers. This dataset is the most comprehensive transcriptome resource currently available for D. antarctica and is therefore expected to be an important foundation for future genetic studies of grasses and extremophiles.

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Response of Phosphoenolpyruvate Carboxylase from Maize Leaves to Moderate Water Deficit

Cited by 5 publications

References 43 publications

Changes to the functional traits of phosphoenolpyruvate carboxylase following hybridization in C‐4 halophytes

Changes to the functional traits of phosphoenolpyruvate carboxylase following hybridization in C‐4 halophytes

From physiology to salt marsh management challenges with sea level rise: the case of native Spartina foliosa, invasive S. densiflora and their hybrid

Transcriptome sequencing of the Antarctic vascular plant Deschampsia antarctica Desv. under abiotic stress

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