Response of rat bone marrow cells to commercially pure titanium submitted to different surface treatments

Xavier, Samuel Porfírio; Carvalho, Paulo Sérgio Perri de; Beloti, Márcio Mateus; Rosa, Adalberto Luiz

doi:10.1016/s0300-5712(03)00027-7

Cited by 68 publications

(72 citation statements)

References 26 publications

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“…Several studies have demonstrated the influence of surface roughness on ALP activity [12,22,35]. We could not, however, corroborate the finding of ALP activity increasing with the roughness parameters, as reported by Kim et al [16], though this discrepancy may be attributed to the short duration (21 days) of our experiments.…”

Section: Discussioncontrasting

confidence: 97%

Enhanced Cell Integration to Titanium Alloy by Surface Treatment with Microarc Oxidation: A Pilot Study

Lim

Kwon

Sun

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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Microarc oxidation (MAO) is a surface treatment that provides nanoporous pits, and thick oxide layers, and incorporates calcium and phosphorus into the coating layer of titanium alloy. We presumed such modification on the surface of titanium alloy by MAO would improve the ability of cementless stems to osseointegrate. We therefore compared the in vitro ability of cells to adhere to MAOed titanium alloy to that of two different types of surface modifications: machined and grit-blasted. We performed energy-dispersive x-ray spectroscopy and scanned electron microscopy investigations to assess the structure and morphology of the surfaces. Biologic and morphologic responses to osteoblast cell lines (SaOS-2) were then examined by measuring cell proliferation, cell differentiation (alkaline phosphatase activity), and avb3 integrin. The cell proliferation rate, alkaline phosphatase activity, and cell adhesion in the MAO group increased in comparison to those in the machined and grit-blasted groups. The osteoblast cell lines of the MAO group were also homogeneously spread on the surface, strongly adhered, and well differentiated when compared to the other groups. This method could be a reasonable option for treating the surfaces of titanium alloy for better osseointegration.

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Section: Discussioncontrasting

confidence: 97%

Enhanced Cell Integration to Titanium Alloy by Surface Treatment with Microarc Oxidation: A Pilot Study

Lim

Kwon

Sun

et al. 2009

Clinical Orthopaedics &Amp; Related Research

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show abstract

“…Relevant differences were observed in this study of cellular adhesion, as the Control Group showed greater adhesion in relation to Groups Exp1 and Exp2. This fact diverges from some studies 17 . However, it is believed that such divergence occurred due to the use of the titanium discs as control groups in these previous studies, whereas the present study used the polystyrene bottom of the plate, which presents considerable cellular adhesion, for the control group.…”

Section: Discussioncontrasting

confidence: 99%

“…Therefore, alkaline phosphatase would be the first specific marker that would be linked to osteogenesis, followed by osteopontin and osteocalcin. Several studies have used the presence of alkaline phosphatase to indicate the presence or absence of mineralization on titanium surfaces 17,23,24 . In a study done using chemical acid and heat treatment (control group) and pure chemical acid (test group) on titanium surfaces, alkaline phosphatase activity was analyzed.…”

mentioning

confidence: 99%

Biological characterization of implant surfaces - in vitro study

Soares

Moura

Júnior

et al. 2015

Rev. odontol. UNESP

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<title>Abstract</title><sec><title>Objective</title>Evaluate the biological performance of titanium alloys grade IV under different surface treatments: sandblasting and double etching (Experimental surface 1; Exp1, NEODENT); surface with wettability increase (Experimental surface 2; Exp2, NEODENT) on response of preliminary differentiation and cell maturation.</sec><sec><title>Material and method</title>Immortalized osteoblast cells were plated on Exp1 and Exp2 titanium discs. The polystyrene plate surface without disc was used as control group (C). Cell viability was assessed by measuring mitochondrial activity (MTT) at 4 and 24 h (n = 5), cell attachment was performed using trypan blue exclusion within 4 hours (n = 5), serum total protein and alkaline phosphatase normalization was performed at 4, 7 and 14 days (n = 5). Data were analyzed using one-way ANOVA and Tukey test.</sec><sec><title>Result</title>The values of cell viability were: 4h: C– 0.32±0.01A; Exp1– 0.34±0.08A; Exp2– 0.29±0.03A. 24h: C– 0.43±0.02A; Exp1– 0.39±0.01A; Exp2– 0.37±0.03A. The cell adhesion counting was: C– 85±10A; Exp1- 35±5B; Exp2– 20±2B. The amounts of serum total protein were 4d: C– 40±2B; Exp1– 120±10A; Exp2– 130±20A. 7d: C– 38±2B; Exp1– 75±4A; Exp2– 70±6A. 14 d: C– 100±3A; Exp1– 130±5A; Exp2– 137±9A. The values of alkaline phosphatase normalization were: 4d: C– 2.0±0.1C; Exp1– 5.1±0.8B; Exp2– 9.8±2.0A. 7d: C– 1.0±0.01C; Exp1– 5.3±0.5A; Exp2– 3.0±0.3B. 14 d: C– 4.1±0.3A; Exp1– 4.4±0.8A; Exp2– 2.2±0.2B. Different letters related to statistical differences.</sec><sec><title>Conclusion</title>The surfaces tested exhibit different behavior at dosage of alkaline phosphatase normalization showing that the Exp2 is more associated with induction of cell differentiation process and that Exp1 is more related to the mineralization process.</sec>

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“…5 The results showed that different treatments for titanium produced different surface topographies, as first indicated by Xavier et al, 14 , significant differences in surface roughness were obtained.…”

Section: Discussionmentioning

confidence: 67%

The Impact of Different Acids Etch on Sandblasted Titanium Dental Implant Surfaces Topography

Al‐Radha¹

2016

IOSR

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Response of rat bone marrow cells to commercially pure titanium submitted to different surface treatments

Cited by 68 publications

References 26 publications

Enhanced Cell Integration to Titanium Alloy by Surface Treatment with Microarc Oxidation: A Pilot Study

Enhanced Cell Integration to Titanium Alloy by Surface Treatment with Microarc Oxidation: A Pilot Study

Biological characterization of implant surfaces - in vitro study

The Impact of Different Acids Etch on Sandblasted Titanium Dental Implant Surfaces Topography

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