Response of selected microbial strains and their consortia to the presence of automobile paints: Biofilm growth, matrix protein content and hydrolytic enzyme activity

Jakovljević, Violeta D.; Radojević, Ivana D.; Grujić, Sandra; Ostojić, Аleksandar

doi:10.1016/j.sjbs.2022.103347

Cited by 2 publications

(1 citation statement)

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“…The main role of AMB Media carriers (Assisting Moving Bad Media) is to increase the active surface area occupied by microorganisms. The carriers used in this study are made of high density polyethylene ( q = 1 g/cm 3 ) and had the following sizes: outer diameter of 12 mm, length of 13 mm, usable internal surfaces of 500 m 2 /m 3 and free areas of 85% ( Jakovljević et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Finding the best combination of autochthonous microorganisms with the most effective biosorption ability for heavy metals removal from wastewater

Jakovljević

Grujić²,

Simić³

et al. 2022

Front. Microbiol.

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The presence of heavy metals (HMs) in the environment represents a serious environmental problem. In this regard, this work was conceived with the aim of finding, among indigenous microorganisms, the species and their combinations with the best biosorption activity for the following HMs: zinc, lead, cadmium, copper, and nickel. The experiment was carried out in several steps: (1) isolation and identification of microbial strains from the Central Effluent Treatment Plant’s wastewater; (2) studying the interaction of microorganisms and the ability to form biofilms in 96-well plates; (3) testing the resistance of biofilms to HMs; (4) testing the growth of biofilms on AMB media carriers in the presence of HMS; and (5) biosorption assay. The selected strains used in this study were: Enterobacter cloacae, Klebsiella oxytoca, Serratia odorifera, and Saccharomyces cerevisiae. The best biofilm producers in control medium were K. oxytoca/S. odorifera (KS), followed by K. oxytoca/S. odorifera/S. cerevisiae (KSC), and E. cloacae/K. oxytoca/S. odorifera (EKS) after 10 days of incubation. Mixed cultures composed of three species showed the highest resistance to the presence of all tested metals. The best biosorption capacity was shown by KSC for Cu2+ (99.18%), followed by EKS for Pb2+ (99.14%) and Cd2+ (99.03%), K. oxytoca for Ni2+ (98.47%), and E. cloacae for Zn2+ (98.06%). This research offers a novel approach to using mixed biofilms for heavy metal removal processes as well as its potential application in the bioremediation of wastewater.

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Section: Methodsmentioning

confidence: 99%