Response of selected poultry cecal probiotic bacteria and a primary poultry<i>salmonella typhimurium</i>isolate grown with or without glucose in liquid batch culture

Durant, J.A.; Nisbet, David J.; Ricke, Steven C.

doi:10.1080/03601230009373286

Cited by 8 publications

(5 citation statements)

References 20 publications

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“…and Veillonella sp. fermenting sugars to acetate and propionate at pH 5.0 under batch conditions did not inhibit serovar Typhimurium growth (8). These findings are in contrast to our findings which showed that relatively low concentrations of propionate (ϳ20 mM) inhibit growth of serovar Enteritidis.…”

Section: Discussioncontrasting

confidence: 57%

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Competitive Exclusion of Salmonella enterica serovar Enteritidis by Lactobacillus crispatus and Clostridium lactatifermentans in a Sequencing Fed-Batch Culture

Wielen

Lipman

Knapen

et al. 2002

Appl Environ Microbiol

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Competitive exclusion of Salmonella enterica serovar Enteritidis by a mixed culture of Lactobacillus crispatus and Clostridium lactatifermentans was studied in a sequencing fed-batch reactor mimicking the cecal ecophysiology of broiler chickens. Growth of serovar Enteritidis was inhibited by a mixed culture of L. crispatus and C. lactatifermentans at pH 5.8 but not by a monoculture of L. crispatus at the same pH. Moreover, experiments performed at pH 7.0 did not show growth inhibition of serovar Enteritidis. L. crispatus fermented lactose to lactate, and C. lactatifermentans fermented the lactate to acetate and propionate in a mixed culture of L. crispatus and C. lactatifermentans growing on lactose. In contrast, only lactate was produced from lactose by a monoculture of L. crispatus. At pH 5.8 considerable concentrations of acetate and propionate were present as undissociated acids, whereas only trace levels of undissociated lactate were present at pH 5.8 due to the low pK a of lactate. At pH 7.0 all three acids were present in their dissociated forms. We conclude that a mixed culture of L. crispatus and C. lactatifermentans inhibits growth of serovar Enteritidis under cecal growth conditions. The undissociated forms of acetate and propionate produced in the mixed culture inhibited the growth of serovar Enteritidis.

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Section: Discussioncontrasting

confidence: 57%

“…This indicates that the sequencing fed-batch reactor might be a good model for studying bacterial interactions as they could occur in the ceca of broilers. In contrast, other in vitro experiments were not performed under cecal ecophysiological conditions (8,10,12,13).…”

Section: Discussionmentioning

confidence: 99%

Competitive Exclusion of Salmonella enterica serovar Enteritidis by Lactobacillus crispatus and Clostridium lactatifermentans in a Sequencing Fed-Batch Culture

Wielen

Lipman

Knapen

et al. 2002

Appl Environ Microbiol

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Section: Introductionmentioning

confidence: 99%

“…2,14 Several studies have explored the physiological functions of Veillonella in maintaining the dynamic balance between lactic acid and SCFAs and its relevance to host health and disease. 15,16 A few studies have observed lactic acid accumulation in the medium when Veillonella strains are cocultured with Enterococcus faecium 17 or found that the medium cultured with Lactobacillus and Veillonella isolated from chicken cecum inhibits pathogenic intestinal bacteria. 18 One of the crucial functions of Veillonella is the fermentation of organic acids to produce SCFAs; while some Veillonella strains cannot ferment carbohydrates such as glucose and lactose, they can ferment organic acids such as pyruvate and lactic acid into acetic acid and propionic acid.…”

Section: Introductionmentioning

confidence: 99%

Cooperative interactions between Veillonella ratti and Lactobacillus acidophilus ameliorate DSS-induced ulcerative colitis in mice

Li,

Wang,

Zhao

et al. 2023

Food Funct.

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“…Chicks are the most susceptible to colonisation by pathogens during the early period, when a stable gut microflora is not established yet, and the stabilised gut microflora provides broiler chicks with vital protection against these undesirable organisms (Chambers and Lu, 2002). The probable reason why young broiler chickens are especially susceptible to Salmonella infection is the lack of a protective microflora in their gastrointestinal tract (Durant et al, 2000).…”

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confidence: 99%

Antimicrobial activity of Enterococcus faecium EF 55 against Salmonella Enteritidis in chicks

Levkut

Pistl

Lauková

et al. 2009

Acta Veterinaria Hungarica

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The protective effect of Enterococcus faecium EF 55 against Salmonella enterica serovar Enteritidis phage type 4 (SE PT4) was studied in 1-day-old chicks. The EF 55 strain (isolated and characterised by the authors earlier) was applied daily (1.10 9 CFU/0.2 ml PBS) for 7 days. Oral inoculation of the SE PT4 strain was performed on day 8 in a single dose of 5.10 8 CFU/0.2 ml PBS. The experiment lasted for 21 days. Samples were collected on day 1 of the experiment to verify the absence of Salmonella, on day 8 to check colonisation of EF 55 and immunological status in experimental birds, and on days 2, 4, 6, 8 and 14 after SE PT4 infection of chicks. Strain EF 55 sufficiently colonised the digestive tract of chicks after 7 days of application. The highest numbers of EF 55 in the faeces of chicks were observed before SE infection and persisted to day 6 post infection (p.i.) in both the EF and EF+SE groups. PCR confirmed the identity of the EF 55 strain. The counts of SE PT4 strain in faeces of the EF+SE group were significantly reduced in comparison to those in the SE group on days 2 and 14 p.i. (P < 0.01). The significant reduction of salmonellae in the caecum was recorded at the end of the experiment (day 14 p.i.) in the EF+SE group in comparison to the SE group (P < 0.01). At day 4 p.i., colonies of S. Enteritidis PT4 were found in the liver of chicks of the SE group in a higher concentration than in chicks of the EF+SE group (P < 0.001). Salmonellae were isolated from the liver until days 8 and 6 p.i. in the SE and EF+SE groups, respectively. The mean values of actual lymphocyte subpopulations in the blood and the relative percentage of caecal intraepithelial lymphocyte subpopulations (CD4, CD8, CD44, TCR, MHC II and IgM) were not influenced at a statistically significant level by the application of the EF 55 and/or the SE PT4 strain. The results demonstrate the antimicrobial effect of E. faecium EF 55 against S. Enteritidis PT4.

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Response of selected poultry cecal probiotic bacteria and a primary poultrysalmonella typhimuriumisolate grown with or without glucose in liquid batch culture

Cited by 8 publications

References 20 publications

Competitive Exclusion of Salmonella enterica serovar Enteritidis by Lactobacillus crispatus and Clostridium lactatifermentans in a Sequencing Fed-Batch Culture

Competitive Exclusion of Salmonella enterica serovar Enteritidis by Lactobacillus crispatus and Clostridium lactatifermentans in a Sequencing Fed-Batch Culture

Cooperative interactions between Veillonella ratti and Lactobacillus acidophilus ameliorate DSS-induced ulcerative colitis in mice

Antimicrobial activity of Enterococcus faecium EF 55 against Salmonella Enteritidis in chicks

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