Paleosols and ichnofossils of the Late Pennsylvanian to Early Permian Monongahela and Dunkard groups of southeastern Ohio provide significant paleoenvironmental and paleoclimatic data related to the Late Paleozoic climatic transition. Forty paleosol profiles from three outcrops in southeastern Ohio were investigated. Methods included the description of paleosol profiles in the field and the analysis of thin sections. Clay mineralogy and bulk geochemistry were used to assess weathering processes and paleoprecipitation. Results, such as the distribution, abundance, and size of physical features including slickensides, pedogenic carbonates, and rhizoliths suggest erratic fluctuations between mildly seasonal wet environments and strongly seasonal dry environments throughout the studied section which are supported by oscillations in calculated paleoprecipitation values. Paleosols of the Monongahela Group are interpreted as mildly to strongly seasonal, wet to wetdry, sparsely to heavily vegetated fen, dry woodland, clastic marsh, early successional vegetation, and brakeland ecosystems forming on proximal to distal floodplain and shoreline lacustrine environments. Paleosols of the Lower Dunkard Group are interpreted as a mildly to strongly seasonal, wet to wet-dry, sparsely to heavily vegetated, clastic marsh, early successional vegetation, and brakeland ecosystems forming on proximal to distal floodplain and distal levee environments. Variability in climate has profound effects on paleolandscapes. By investigating changes in paleosols through episodes of climatic transition, we can better understand the impact these changes have on soils and soil ecosystems of the past and future.