Response of sugarcane (Saccharum species hybrid) genotypes to embryogenic callus induction and in vitro salt stress

Badawy, O. M.; Nasr, M. I.; Alhendawi, Ramadan A.

doi:10.1007/s12355-008-0043-8

Cited by 17 publications

(10 citation statements)

References 18 publications

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“…Identifying the genetic variation among sugarcane genotypes was also examined via the RAPD-PCR analysis. Our results confirm previous findings on sugarcane ( Badawy et al, 2008 ) and rice ( Van Sanford et al, 2001 ), as they found that callus induction capacity is genotype-dependent. Additionally, Errabii et al (2007) tested the sugarcane callus with MS medium with some stress factors and reported that these stresses reduced the RGR for all the tested genotypes.…”

Section: Discussionsupporting

confidence: 93%

Callus induction and regeneration in sugarcane under drought stress

Abdelsalam

Grad

Ghura

et al. 2021

Saudi Journal of Biological Sciences

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Tissue culture methods are useful in assessing the tolerance of various stresses due to the ease of controlling stress under in vitro conditions. This study aimed to investigate the response of sugarcane genotyps to drought stress using calli as a model system. For inducing sugarcane callus, the medium of Murashige and Skoog (MS) was used with different mannitol concentrations (100, 200, and 300 mM) to measure their effects on callus frequency, the day of callus initiation, embryogenic potential, relative growth rate (RGR), water and proline contents, K + and Na + contents, as well as the formation of shoot and roots for three sugarcane genotypes (e.g., GT 54-9, G 84-47, and pH 8013). The RAPD-PCR analysis was carried out using five oligonucleotide primers to identify the genetic variation among sugarcane genotypes. The results indicated that the degree of callus proliferation varied from 70 − 86%. The highest value of callus proliferation, PGR, shoot formation was recorded for the genotype GT 54-9 compared to the other two genotypes (G 84-47 and pH 8013). Calli treated with 100 mM mannitol showed the highest RGR, proline and waer contents for the genotype GT 54-9, while, those treated with 300 mM recorded the lowest values of these parameters for the genotype pH 8013. The genotype G 84-47 collected highest Na + content, while the genotype pH 8013 collected highest K + content. The results of this study recommend preference for GT 54-9 genotype, which is considered the most promising genotype, showing more tolerance to drought stress based on all studied traits.

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Section: Discussionsupporting

confidence: 93%

Callus induction and regeneration in sugarcane under drought stress

Abdelsalam

Grad

Ghura

et al. 2021

Saudi Journal of Biological Sciences

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“…Callus initiation was observed at all levels of 2,4-D in both explants; however, it varied with the medium used and the explant source, ranging from 73.33 to 96.67% with leaf and from 56.67 to 86.67% with pith explant. The highest callus induction rate, i.e., 96.67%, was noted on leaf explants with 3.0 mg/L 2,4-D. Gill et al (2002) and Badawy et al (2008) have also reported similar results. Using pith as explants, the highest callus induction rate, i.e., 86.67%, was recorded at 1.0 mg/L and the lowest (56.67%) at 9.0 mg/L.…”

Section: Callogenesis In Response To 24-d In Ms Mediummentioning

confidence: 56%

Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR

Shahid¹,

Khan²,

Saeed³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Sugarcane breeding under climatic conditions of Pakistan is very difficult due to unavailability of viable fuzz (seed). Somaclonal variation can provide an alternative for improvement of existing genotypes. Six hundred and twenty-seven somaclones were developed from sugarcane genotype S97US297, and protocols for callogenesis and organogenesis were developed using Murashige and Skoog medium. Two types of explants, leaf and pith, and two auxins, 2,4-dichlorophenoxy acetic acid and indole-3-acetic acid, were tested to optimize callogenesis for root establishment. Leaves as explants with 3.0 mg/L 2,4-dichlorophenoxy acetic acid gave the best results, both for callus induction and proliferation. Half-strength Murashige and Skoog medium with 1.5 mg/L indole-3-butyric acid proved to be the best for rooting. Red rot-resistant somaclones of the R 2 generation along with the parent were assessed for genetic variability at the molecular level using RAPD and SSR markers. Polymorphism based on RAPD and SSR was 32 and 67%, respectively. Polymorphic information content ranged from 0.06-0.45 for RAPD and 0.06-0.47 for SSR. We conclude that somaclonal variation of sugarcane varieties is sufficient to allow selection.

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“…Many scientists have used 2, 4-D for callus formation and found it effective. Like Ather et al (2009) obtained 100% callus induction in 3.0 mg/L of 2,4-D. Badawy et al (2008), Pandey et al (2011) and Gandonou et al (2005a) worked on callus inductions of sugarcane using different harmonal levels and found satisfactory results at 3.0 mg/L of 2,4-D. In contrast, Eldessoky et al 2011used sugarcane GT54-9 (C9) cultivar and obtained best results producing embryonic calli at 4 mg/L 2,4-D.…”

Section: Effects Of Auxin (24-d) Levels On Cifmentioning

confidence: 76%

In vitro regeneration, detection of somaclonal variation and screening for mosaic virus in sugarcane (Saccharum spp.) somaclones

Smiullah¹

2012

Afr. J. Biotechnol.

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Three sugarcane accessions susceptible to sugarcane mosaic virus; HSF-240, S-2000-US-359, and S-2003-US-704 were evaluated for callogenesis and regeneration ability. For callogenesis, five different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) was used. The best callogenesis was obtained when Murashige and Skoog (MS) was portified with 3 mg/L 2,4-D and the highest regeneration was obtained on media containing MS + kinetin 0.5 + 0.5 mg/L naphthalene acetic acid (NAA). After succesful regeneration and rooting on half strength MS medium, with 1.5 mg/L indole-3-butyric acid supplementation, plantlets were shifted to green house. Enzyme linked immunosorbent assay (ELISA) test was performed to detect the presence of sugarcane mosaic virus (SCMV) in the regenerated plantlets and simple sequence repeat (SSR) markers were used to evaluate the genetic variation at DNA level between the parent's plants and regenerated somaclones of the accession HSF-240. A total of 26 parent plants and 64 somaclones, among the regenerated plants were selected for the screening of virus through double antibody sandwich (DAS-ELISA) test. Four (4) parent plants out of the 26, showed negative reaction to the virus test. Ten (10) somaclones showed positive reaction to the disease, 9 somaclones showed mild reaction to virus and 45 somaclones showed negative reaction. For the detection of somaclonal variation, 38 primers pair were used and 15 simple sequence repeats (SSR) primer pairs were found to be polymorphic with 51.61% polymorphism. The study demonstrates that SSR genetic markers are the best tool for the investigation of genetic variation in sugarcane.

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Response of sugarcane (Saccharum species hybrid) genotypes to embryogenic callus induction and in vitro salt stress

Cited by 17 publications

References 18 publications

Callus induction and regeneration in sugarcane under drought stress

Callus induction and regeneration in sugarcane under drought stress

Variability of red rot-resistant somaclones of sugarcane genotype S97US297 assessed by RAPD and SSR

In vitro regeneration, detection of somaclonal variation and screening for mosaic virus in sugarcane (Saccharum spp.) somaclones

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