Response of the Anaerobic Methanotroph “
            <i>Candidatus</i>
            Methanoperedens nitroreducens” to Oxygen Stress

Guerrero-Cruz, Simon; Cremers, Geert; Alen, Theo A. van; Camp, Huub J. M. Op den; Jetten, Mike S. M.; Rasigraf, Olivia; Vaksmaa, Annika

doi:10.1128/aem.01832-18

Cited by 56 publications

(37 citation statements)

References 102 publications

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“…Analysis of the microbial community in the Italian paddy field ANME-2d enrichment using a bioreactor approach has been described by Guerrero-Cruz et al . 2018. Metagenome sequencing of the DNA derived from this bioreactor revealed that 83% of 16S rRNA gene reads were assigned to Ca .…”

Section: Resultsmentioning

confidence: 95%

“…Growth conditions and operation of the bioreactor containing ANME-2d archaea enriched from an Italian paddy field soil are described by Guerrero-Cruz et al . (2018). Sampled material from both reactors was centrifuged (10000 × g , 20 min, 4°C) and pellets were kept at −80°C until subsequent freeze-drying and following lipid and isotope analysis.…”

Section: Methodsmentioning

confidence: 99%

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Anaerobic methanotrophic archaea of the ANME-2d clade feature lipid composition that differs from other ANME archaea

Kurth

Smit

Berger

et al. 2019

FEMS Microbiology Ecology

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The anaerobic oxidation of methane (AOM) is a microbial process present in marine and freshwater environments. AOM is important for reducing the emission of the second most important greenhouse gas methane. In marine environments anaerobic methanotrophic archaea (ANME) are involved in sulfate-reducing AOM. In contrast, Ca. Methanoperedens of the ANME-2d cluster carries out nitrate AOM in freshwater ecosystems. Despite the importance of those organisms for AOM in non-marine environments little is known about their lipid composition or carbon sources. To close this gap, we analysed the lipid composition of ANME-2d archaea and found that they mainly synthesise archaeol and hydroxyarchaeol as well as different (hydroxy-) glycerol dialkyl glycerol tetraethers, albeit in much lower amounts. Abundant lipid headgroups were dihexose, monomethyl-phosphatidyl ethanolamine and phosphatidyl hexose. Moreover, a monopentose was detected as a lipid headgroup that is rare among microorganisms. Batch incubations with 13C labelled bicarbonate and methane showed that methane is the main carbon source of ANME-2d archaea varying from ANME-1 archaea that primarily assimilate dissolved inorganic carbon (DIC). ANME-2d archaea also assimilate DIC, but to a lower extent than methane. The lipid characterisation and analysis of the carbon source of Ca. Methanoperedens facilitates distinction between ANME-2d and other ANMEs.

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Section: Resultsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Anaerobic methanotrophic archaea of the ANME-2d clade feature lipid composition that differs from other ANME archaea

Kurth

Smit

Berger

et al. 2019

FEMS Microbiology Ecology

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“…Interestingly, Candidatus Methanoperedens, mediating nitrate-dependent anaerobic CH 4 oxidation, was also typically representative of these oxygen-rich environments, although it was found to be relatively more abundant within the deeper sampling depth. This genus can counteract oxidative damage and adapt in environments where it is regularly exposed to oxygen [52].…”

Section: 32methane Oxidizing Microorganismsmentioning

confidence: 99%

Abundance and biogeography of methanogenic and methanotrophic microorganisms across European streams

Nagler

Praeg

Niedrist

et al. 2019

Preprint

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Background: Globally, streams emit significant amounts of methane, a highly potent greenhouse gas. However, little is known about the stream sediment microbial communities that control the net methane balance in these systems, and in particular about their distribution and composition at large spatial scales. This study investigated the diversity and abundance of methanogenic archaea and methane-oxidizing microorganisms across 16 European streams (from northern Spain to northern Sweden and from western Ireland to western Bulgaria) via 16S rRNA gene sequencing and qPCR. Furthermore, it examined environmental factors influencing both abundance and community composition and explored the link to measured potential methane production and oxidation rates of the respective sediments. Results: Our results demonstrated that the methanogenic and methanotrophic microbiomes of the studied European streams were linked to both the temperature and degree of anthropogenic alteration. The microbiomes could be separated into two to three groups according to environmental factors at both stream and catchment scales. Main methanogenic taxa found within more anthropogenically-altered, warm, and oxygen-poor environments were either Methanospirillum spp. or members of the families Methanosarcinaceae and Methanobacteriaceae . Within such environments, methane oxidizing communities were strongly characterized by members of the family Methylobacteriaceae ( Meganema spp. and Microvirga spp.). Contrastingly, communities in colder environments rich in oxygen and with relatively little anthropogenic impact at the catchment scale were characterized by the methanogenic Methanosaetaceae , Methanocellaceae and Methanoregulaceae and the methanotrophic Methyloglobulus spp ., members of the CABC2E06 group (all Methylococcaceae ) and by various Candidatus Methanoperedens. Overall, diversity of methanogenic archaea increased with increasing water temperature. Methane oxidizing communities showed higher diversities in southern sampling sites and in streams with larger stream areas and widths. Potential methane production rates significantly increased with increasing abundance of methanogenic archaea, while potential methane oxidation rates did not show significant correlations with abundances of methane oxidizing bacteria, presumably due to the more diverse physiological capabilities of this group. Conclusions: We present the first large scale overview of the large-scale microbial biogeography of two microbial groups driving the methane cycle dynamics within stream sediments and deduce the impact that future anthropogenic alterations may cause.

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“…Furthermore, ‘ Ca . Methanoperedens’ might be more sensitive to oxygen exposure (Guerrero-Cruz et al 2018 ) than anammox and ‘ Ca . Methylomirabilis’ bacteria (Luesken et al 2011a , 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…Methylomirabilis’ as well as anammox is dependent on nitrite. Oxygen is detrimental for all three processes (Strous et al 1999b ; Luesken et al 2012 ; Guerrero-Cruz et al 2018 ). Although several studies have enriched combinations of N-damo bacteria and archaea or N-damo archaea and anammox bacteria, few studies have been designed to enrich all three groups from the same source material (Arshad et al 2017 ; Stultiens et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant

Stultiens

Kessel

Frank

et al. 2020

Appl Microbiol Biotechnol

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Anaerobic wastewater treatment offers several advantages; however, the effluent of anaerobic digesters still contains high levels of ammonium and dissolved methane that need to be removed before these effluents can be discharged to surface waters. The simultaneous anaerobic removal of methane and ammonium by denitrifying (N-damo) methanotrophs in combination with anaerobic ammonium-oxidizing (anammox) bacteria could be a potential solution to this challenge. After a molecular survey of a wastewater plant treating brewery effluent, indicating the presence of both N-damo and anammox bacteria, we started an anaerobic bioreactor with a continuous supply of methane, ammonium, and nitrite to enrich these anaerobic microorganisms. After 14 months of operation, a stable enrichment culture containing two types of 'Candidatus Methylomirabilis oxyfera' bacteria and two strains of 'Ca. Brocadia'-like anammox bacteria was achieved. In this community, anammox bacteria converted 80% of the nitrite with ammonium, while 'Ca. Methylomirabilis' contributed to 20% of the nitrite consumption. The analysis of metagenomic 16S rRNA reads and fluorescence in situ hybridization (FISH) correlated well and showed that, after 14 months, 'Ca. Methylomirabilis' and anammox bacteria constituted approximately 30 and 20% of the total microbial community. In addition, a substantial part (10%) of the community consisted of Phycisphaera-related planctomycetes. Assembly and binning of the metagenomic sequences resulted in high-quality draft genome of two 'Ca. Methylomirabilis' species containing the marker genes pmoCAB, xoxF, and nirS and putative NO dismutase genes. The anammox draft genomes most closely related to 'Ca. Brocadia fulgida' included the marker genes hzsABC, hao, and hdh. Whole-reactor and batch anaerobic activity measurements with methane, ammonium, nitrite, and nitrate revealed an average anaerobic methane oxidation rate of 0.12 mmol h −1 L −1 and ammonium oxidation rate of 0.5 mmol h −1 L −1. Together, this study describes the enrichment and draft genomes of anaerobic methanotrophs from a brewery wastewater treatment plant, where these organisms together with anammox bacteria can contribute significantly to the removal of methane and ammonium in a more sustainable way. Key points • An enrichment culture containing both N-damo and anammox bacteria was obtained. • Simultaneous consumption of ammonia, nitrite, and methane under anoxic conditions. • In-depth metagenomic biodiversity analysis of inoculum and enrichment culture.

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Response of the Anaerobic Methanotroph “ Candidatus Methanoperedens nitroreducens” to Oxygen Stress

Cited by 56 publications

References 102 publications

Anaerobic methanotrophic archaea of the ANME-2d clade feature lipid composition that differs from other ANME archaea

Anaerobic methanotrophic archaea of the ANME-2d clade feature lipid composition that differs from other ANME archaea

Abundance and biogeography of methanogenic and methanotrophic microorganisms across European streams

Diversity, enrichment, and genomic potential of anaerobic methane- and ammonium-oxidizing microorganisms from a brewery wastewater treatment plant

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