Response of the Distal Tubule and Cortical Collecting Duct to Vasopressin in the Rat

Woodhall, Philip B.; Tisher, C. Craig

doi:10.1172/jci107509

Cited by 146 publications

(66 citation statements)

References 16 publications

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“…The lack of a clear delineation between each segment of the distal nephron supports previous descriptions of a gradual transition and mixing of tubular segments in the distal nephron of rat, mouse and human ( Figure 6E). [37][38][39][40] The connecting tubule therefore represents a hybrid segment composed of elements from the adjacent metanephric mesenchyme-derived distal tubule and ureteric budderived CD, with the latter nephron segment the mostly likely source of ICs.…”

Section: Discussionmentioning

confidence: 99%

Remodeling of the Fetal Collecting Duct Epithelium

Hiatt

Ivanova

Torán

et al. 2010

The American Journal of Pathology

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Congenital urinary tract obstruction induces changes to the renal collecting duct epithelium, including alteration and depletion of intercalated cells. To study the effects of obstruction on the ontogeny of intercalated cell development, we examined normal and obstructed human fetal and postnatal kidneys. In the normal human fetal kidney, intercalated cells originated in the medullary collecting duct at 8 weeks gestation and remained most abundant in the inner medulla throughout gestation. In the cortex, intercalated cells were rare at 18 and 26 weeks gestation and observed at low abundance at 36 weeks gestation. Although early intercalated cells exhibit an immature phenotype, Type A intercalated cells predominated in the inner and outer medullae at 26 and 36 weeks gestation with other intercalated cell subtypes observed rarely. Postnatally, the collecting duct epithelium underwent a remodeling whereby intercalated cells become abundant in the cortex yet absent from the inner medulla. In 18-week obstructed kidneys with mild to moderate injury, the intercalated cells became more abundant and differentiated than the equivalent age-matched normal kidney. In contrast, more severely injured ducts of the late obstructed kidney exhibited a significant reduction in intercalated cells. These studies characterize the normal ontogeny of human intercalated cell development and suggest that obstruction induces premature remodeling and differentiation of the fetal collecting duct epithelium.

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Section: Discussionmentioning

confidence: 99%

Remodeling of the Fetal Collecting Duct Epithelium

Hiatt

Ivanova

Torán

et al. 2010

The American Journal of Pathology

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“…and [Nab, are the bath and collected fluid concentrations of sodium, respectively. previous micropuncture studies (31). The finding by Woodhall and Tisher (31) of a marked heterogeneity in the cellular composition of the subcapsular tubules available for distal micropuncture makes the interpretation of previous studies problematic.…”

Section: Methodsmentioning

confidence: 98%

“…The epithelium lining tubules just before the junction of one "distal" segment with another, usually referred to in micropuncture studies as the "late distal tubule," was found in all cases to consist of epithelium of the cortical collecting tubule (CCT). ' In the Wistar rat, 48.3% of available tubules were of true distal epithelial type, 45% were collecting tubules, and 6.7% were transition zones (31). Thus, it is clear that many of the previous micropuncture results have 1Abbreviations used in this paper: ADH, antidiuretic hormone; CCT, cortical collecting tubule; DCT, distal convoluted tubule; DOCA, deoxycorticosterone acetate; PD, potential difference.…”

Section: Introductionmentioning

confidence: 96%

“…In addition, indirect information concerning the characteristics of the DCT has been obtained from clearance (20)(21)(22)(23)(24)(25)(26) and from toad bladder experiments (27)(28)(29)(30). However, the recent observations of Woodhall and Tisher raise serious questions about the interpretation of previous micropuncture studies in relation to functional characterization of the DCT (31). These authors found a significant anatomic heterogeneity of surface tubules available for distal puncture when the standard micropuncture definition of the distal tubule was used.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A functional comparison of the cortical collecting tubule and the distal convoluted tubule.

Gross¹,

Imai²,

Kokko³

1975

J. Clin. Invest.

167

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AB STRA CT Electrical and permeability features of the distal convoluted tubule (DCT) and the cortical collecting tubule (CCT) were examined using the technique in which isolated segments of rabbit tubules were perfused in vitro. When rabbits were given a regular diet and tubules were perfused and bathed in artificial solutions simulating plasma ultrafiltrate, the potential difference (PD) was + 3.7+1.9 mV in the CCT and -40.4±2.8 mV in the DCT. When rabbits were given a low sodium, high potassium diet plus i.m. deoxycorticosterone acetate (DOCA) (1 mg/kg per day), the PD in both the CCT (-30.8±3.9 mV) and the DCT (-33.8±5.5 mV) was negative. The PD in the CCT was quantitatively similar to that of diet plus DOCA when animals were given DOCA alone. The PD in both segments was inhibited by ouabain (10' M) in the bath or by amiloride (10' M) in the perfusate. Addition of vasopressin (200 AU/ml) to the bath caused a gradual decline of PD to zero in the CCT but failed to produce a potential response in the DCT. Osmotic water permeability was essentially zero in both segments in the absence of vasopressin. After addition of the vasopressin to the bath, osmotic water permeability in the DCT remained zero but increased to 71.9+25.5 x 10-7 cm/s per atm in the CCT. We conclude that both segments are similar in that each possesses an electrogenic transport process but that these segments differ in that: (a) the CCT requires either exogenous or endogenous mineralocorticoid to maintain a maximal negative PD, whereas the PD in the DCT appears to be independent of mineralocorticoid effect; and (b) the CCT responds to vasopressin with a marked rise in water permeability, whereas the DCT is impermeable to water before and after addition of vasopressin.Dr. Imai's present address is the Department of Pharmacology, Jichi Medical School, Tochigi, Japan 329-04.

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“…The height of kidney tubule cells is often determined as the mean caliper height, the linear distance between the luminal and basolateral membranes on thin sections (8,10,17). This approach is complicated by at least three kinds of problems.…”

Section: Appendixmentioning

confidence: 99%

Adaptation of the distal convoluted tubule of the rat. Structural and functional effects of dietary salt intake and chronic diuretic infusion.

Ellison¹,

Velázquez²,

Wright³

1989

J. Clin. Invest.

261

170

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We studied the effects of dietary NaCl intake on the renal distal tubule by feeding rats high or low NaCl chow or by chronically infusing furosemide. Furosemide-treated animals were offered saline as drinking fluid to replace urinary losses. Effects of NaCI intake were evaluated using free-flow micropuncture, in vivo microperfusion, and morphometric techniques. Dietary NaCI restriction did not affect NaCI delivery to the early distal tubule but markedly increased the capacity of the distal convoluted tubule to transport Na and Cl. Chronic furosemide infusion increased NaCI delivery to the early distal tubule and also increased the rates of Na and Cl transport above the rates observed in low NaCI diet rats. When compared with high NaCI intake alone, chronic furosemide infusion with saline ingestion increased the fractional volume of distal convoluted tubule cells by nearly 100%, whereas dietary NaCI restriction had no effect. The results are consistent with the hypotheses that (a) chronic NaCl restriction increases the transport ability of the distal convoluted tubule independent of changes in tubule structure, (b) high rates of ion delivery to the distal nephron cause tubule hypertrophy, and (c) tubule hypertrophy is associated with increases in ion transport capacity. They indicate that the distal tubule adapts functionally and structurally to perturbations in dietary Na and Cl intake.

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Response of the Distal Tubule and Cortical Collecting Duct to Vasopressin in the Rat

Cited by 146 publications

References 16 publications

Remodeling of the Fetal Collecting Duct Epithelium

Remodeling of the Fetal Collecting Duct Epithelium

A functional comparison of the cortical collecting tubule and the distal convoluted tubule.

Adaptation of the distal convoluted tubule of the rat. Structural and functional effects of dietary salt intake and chronic diuretic infusion.

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