The titanium alloy has been used for marine structrural components because of the excellent properties. The response of material under explosion when in the service process should be carried out. In this research, the explosion test of the Ti6321 titanium of three different structure is executed to study the microstructure evolution. The optical microscope is used for observing the microstructure and adiabatic shear bands (ASB). The electron backscatter diffraction method is used to study the microstructure and recrystallization of the biamodal structural target under different charges. The result shows that the deformation of the target become larger with the increase of the charge. The ASBs have been observed in the target, demonstrating that it is an important reason for the damage. The twinning mechanism comes into play a role in the deformation of the material while dislocations become difficult to satisfy. The grain size of the bimodal material decrease with the increase of the charge. The deformed grains of the target start to recover and recrystallize at high temperature due to shock waves of explosion.