2022
DOI: 10.1128/spectrum.02116-21
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Response of Total (DNA) and Metabolically Active (RNA) Microbial Communities in Miscanthus × Giganteus Cultivated Soil to Different Nitrogen Fertilization Rates

Abstract: Miscanthus × giganteus is a promising candidate for bioeconomy cropping systems; however, it remains unclear how the soil microbiome supplies nitrogen to this low-input crop. DNA-based techniques are used to provide community characterization, but may miss important metabolically active taxa.

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Cited by 6 publications
(2 citation statements)
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“…with potential for protein synthesis) communities in the global tropical and subtropical ocean from the surface to the bathypelagic layer (~4000 m). Active communities differed largely from total communities throughout the water column, as observed in other ecosystems [ 54 , 55 ]. Despite the marked environmental gradients encountered with depth, the active communities were comparatively more similar among them than the total communities were ( Fig.…”
Section: Discussionmentioning
confidence: 83%
“…with potential for protein synthesis) communities in the global tropical and subtropical ocean from the surface to the bathypelagic layer (~4000 m). Active communities differed largely from total communities throughout the water column, as observed in other ecosystems [ 54 , 55 ]. Despite the marked environmental gradients encountered with depth, the active communities were comparatively more similar among them than the total communities were ( Fig.…”
Section: Discussionmentioning
confidence: 83%
“…However, DNA-based methods have limitations in accurately reflecting in situ nitrification rates (Nardi et al, 2022), and the exact microbial groups that contribute to the nitrification function can be masked. To approach true field rates, measurements such as DNA stable isotope probing and RNA-based methods such as metatranscriptomics, are suggested (Blanc-Betes et al, 2023;Yang et al, 2022). Integrated experimental designs are crucial for addressing the inherent complexities of plant-soil-microbe systems and comprehensively characterizing BNI function in different grass species of interest.…”
Section: Biological Nitrification Inhibition In Grassesmentioning
confidence: 99%