Response of transgenic poplar overexpressing cytosolic glutamine synthetase to phosphinothricin

Pascual, María Belén; Jing, Zhong; Kirby, Edward G.; Cánovas, Francisco M.; Gallardo, Fernando

doi:10.1016/j.phytochem.2007.07.031

Cited by 43 publications

(27 citation statements)

References 37 publications

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“…A PPT treatment of the Populus tremula × P. alba hybrid led to a fall of majority of leaves in both control and transgenic plants, though they further diverged from each other in growth rate [14]. In our report, damage symptoms of leaf tissue did not reflect biochemical processes in birch plants after the herbicide treatment.…”

Section: Journal Of Botanycontrasting

confidence: 67%

“…Treatment of 3-week-old Populus tremula × P. alba plants having the pine GS1 gene in a greenhouse with PPT at a dose equivalent to 275 g⋅ha −1 led to fall of most of leaves in both control and transgenic lines, but a net growth in transgenic plants after the treatment was 5-fold higher [14]. However, the PPT treatment at a 500 g⋅ha −1 dose killed all plants during 3 days.…”

Section: Discussionmentioning

confidence: 95%

“…So, Populus tremula × P. alba lines carrying the pine GS1 gene demonstrated higher survivability on media with 5 M PPT as compared to control. It has been attributed to increased GS levels [14]. Sublethal dose of PPT in medium (0.5 mgL −1 ) increases rooting frequency, number of roots, and their length in some aspen lines in vitro, transformed with the pine GS1 gene [4].…”

Section: Discussionmentioning

confidence: 99%

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Lack of Correlation between Ammonium Accumulation and Survival of Transgenic Birch Plants with Pine Cytosolic Glutamine Synthetase Gene after “Basta” Herbicide Treatment

2015

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Transformation of plants with genes encoding a glutamine synthetase (GS), a key nitrogen metabolism enzyme, is usually used to increase productivity. However, overexpression of these genes may increase resistance to phosphinothricin (PPT) that irreversibly inhibits GS causing ammonium accumulation in plant tissues. Transgenic plants of two birch (Betula pubescens) genotypes expressing a pine cytosolic GS gene were used for studying the PPT effect on trees. Two control and 8 transgenic lines were treated with herbicide "Basta" at dose equivalent to 2.5 and 5 Lha −1 . Necrosis and abscission of leaves occurred irrespective of a transgenic status or the treatment dose. Ammonium content in leaf tissue in 3 days after the 5 Lha −1 treatment was substantially increased in all plants, 3.2-16.0 times depending on line. After the 2.5 Lha −1 treatment, ammonium content in three transgenic lines was not different from that in control variant sprayed with water. The herbicide treatment caused more prominent desiccation in the bp3f1 genotype nontransgenic plants as compared to transgenic plants, but not in the bp4a genotype. Lack of correlation between ammonium levels and survival of transgenic plants suggests that ammonium toxicity is not a main reason for the birch plant death after the PPT treatment.

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Section: Journal Of Botanycontrasting

confidence: 67%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Lack of Correlation between Ammonium Accumulation and Survival of Transgenic Birch Plants with Pine Cytosolic Glutamine Synthetase Gene after “Basta” Herbicide Treatment

2015

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“…Compared to the NT plants, the GS 1 transformants grew for extended periods and exhibited delayed flowering when grown under both LN and HN. While there are many reports of overexpression of GS 1 in different plants in the literature, the outcomes are very varied (Eckes et al 1989;Temple et al 1993;Oliveira et al 2002;Ortega et al 2004;Kirby et al 2006;Pascual et al 2008). Poplar plants transformed with a pine GS 1 gene, showed increased growth in height (Gallardo et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Impact of concurrent overexpression of cytosolic glutamine synthetase (GS1) and sucrose phosphate synthase (SPS) on growth and development in transgenic tobacco

Seger

Gebril

Tabilona

et al. 2014

Planta

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The outcome of simultaneously increasing SPS and GS activities in transgenic tobacco, suggests that sucrose is the major determinant of growth and development, and is not affected by changes in N assimilation. Carbon (C) and nitrogen (N) are the major components required for plant growth and the metabolic pathways for C and N assimilation are very closely interlinked. Maintaining an appropriate balance or ratio of sugar to nitrogen metabolites in the cell, is important for the regulation of plant growth and development. To understand how C and N metabolism interact, we manipulated the expression of key genes in C and N metabolism individually and concurrently and checked for the repercussions. Transgenic tobacco plants with a cytosolic soybean glutamine synthetase (GS1) gene and a sucrose phosphate synthase (SPS) gene from maize, both driven by the CaMV 35S promoter were produced. Co-transformants, with both the transgenes were produced by sexual crosses. While GS is the key enzyme in N assimilation, involved in the synthesis of glutamine, SPS plays a key role in C metabolism by catalyzing the synthesis of sucrose. Moreover, to check if nitrate has any role in this interaction, the plants were grown under both low and high nitrogen. The SPS enzyme activity in the SPS and SPS/GS1 co-transformants were the same under both nitrogen regimens. However, the GS activity was lower in the co-transformants compared to the GS1 transformants, specifically under low nitrogen conditions. The GS1/SPS transformants showed a phenotype similar to the SPS transformants, suggesting that sucrose is the major determinant of growth and development in tobacco, and its effect is only marginally affected by increased N assimilation. Sucrose may be functioning in a metabolic capacity or as a signaling molecule.

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“…Herbicide resistance may be due to over-production and accumulation of GS, the target enzyme (Behrmann et al, 1990;Pascual et al, 2007)-a manifestation of quantitative, adaptive tolerance. LGlutamate grown SRPPT mycelia showed only a marginal increase in the glutamine synthetase specific activity (Table 3).…”

Section: Occurrence Of Spontaneous Ppt Resistance In a Nigermentioning

confidence: 99%

Phosphinothricin resistance in Aspergillus niger and its utility as a selectable transformation marker

Ahuja

Punekar

2008

Fungal Genetics and Biology

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Response of transgenic poplar overexpressing cytosolic glutamine synthetase to phosphinothricin

Cited by 43 publications

References 37 publications

Lack of Correlation between Ammonium Accumulation and Survival of Transgenic Birch Plants with Pine Cytosolic Glutamine Synthetase Gene after “Basta” Herbicide Treatment

Lack of Correlation between Ammonium Accumulation and Survival of Transgenic Birch Plants with Pine Cytosolic Glutamine Synthetase Gene after “Basta” Herbicide Treatment

Impact of concurrent overexpression of cytosolic glutamine synthetase (GS1) and sucrose phosphate synthase (SPS) on growth and development in transgenic tobacco

Phosphinothricin resistance in Aspergillus niger and its utility as a selectable transformation marker

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