Response of turkey pectoralis major muscle satellite cells to hot and cold thermal stress: Effect of growth selection on satellite cell proliferation and differentiation

Xu, Jiahui; Strasburg, Gale M.; Reed, Kent M.; Velleman, Sandra G.

doi:10.1016/j.cbpa.2020.110823

Cited by 23 publications

(77 citation statements)

References 66 publications

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“…During the immediate 7 days after hatch, SCs exhibit peak mitotic activity (Mozdziak et al, 1994;Halevy et al, 2000) and responsiveness to extrinsic temperatures (Halevy et al, 2001;Patael et al, 2019;Xu et al, 2021). Furthermore, selection for growth has increased the proliferation (Merly et al, 1998;Velleman et al, 2000) and thermal sensitivity (Clark et al, 2016;Xu et al, 2021) of pmSCs. Rossi et al (2010) showed that faster-proliferating SCs have a greater potential to spontaneously express adipogenic characteristics than slowerproliferating populations.…”

Section: Discussionmentioning

confidence: 99%

“…The proliferation rate and differentiation level of pmSCs in growthselected faster-growing turkeys is more responsive to both heat and cold stress than non-selected slower-growing turkeys (Clark et al, 2016;Xu et al, 2021). In addition, proliferating pmSCs are more sensitive to both heat and cold stress than differentiating pmSCs (Xu et al, 2021).…”

Section: Introductionmentioning

confidence: 92%

“…As multipotential stem cells (Asakura et al, 2001;Shefer et al, 2004), SCs with appropriate external stimuli have the potential to spontaneously convert to other lineages such as adipocytes (Asakura et al, 2001;Csete et al, 2001;Shefer et al, 2004;Redshaw and Loughna, 2012). Environmental temperature is an external stimulus that has been shown to alter the cellular function and fate of SCs in chickens (Halevy et al, 2001;Harding et al, 2015;Piestun et al, 2017;Patael et al, 2019) and turkeys (Clark et al, 2016(Clark et al, , 2017Xu et al, 2021). Piestun et al (2017) and Patael et al (2019) showed when chickens were challenged with chronic heat stress immediately after hatch for 2 weeks, proliferation of SCs was suppressed while fat deposition was increased in the pectoralis major (p. major; breast) muscle.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, when exposed to thermal stress, fastergrowing poultry have reduced thermal tolerance (Berrong and Washburn, 1998;Yahav, 2000;Chiang et al, 2008), resulting in prolonged negative effects on breast muscle structure and growth (Joiner et al, 2014;Piestun et al, 2017;Patael et al, 2019). The proliferation rate and differentiation level of pmSCs in growthselected faster-growing turkeys is more responsive to both heat and cold stress than non-selected slower-growing turkeys (Clark et al, 2016;Xu et al, 2021). In addition, proliferating pmSCs are more sensitive to both heat and cold stress than differentiating pmSCs (Xu et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…The commercial turkeys used in this study were selected for growth characteristics including high growth rate and heavy breast muscling, whereas the RBC2 turkeys represent commercial turkeys in the mid-1960s (Nestor et al, 1969) and are slower-growing turkeys without heavy breast muscling. The temperature regimens used in the current study included all the possible combinations of heat and cold stress during pmSC proliferation and/or differentiation as reported by Xu et al (2021). These regimens allowed a complete assessment of the effect of thermal stress on the adipogenic characteristics of the pmSCs from the two genetic lines.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Temperature and Selection for Growth on Intracellular Lipid Accumulation and Adipogenic Gene Expression in Turkey Pectoralis Major Muscle Satellite Cells

Strasburg

Reed

et al. 2021

Front. Physiol.

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As multipotential stem cells, satellite cells (SCs) have the potential to express adipogenic genes resulting in lipid synthesis with thermal stress. The present study determined the effect of temperature on intracellular lipid synthesis and adipogenic gene expression in SCs isolated from the pectoralis major (p. major) muscle of 7-day-old fast-growing modern commercial (NC) turkeys compared to SCs from unselected slower-growing turkeys [Randombred Control Line 2 (RBC2)]. Since proliferating and differentiating SCs have different responses to thermal stress, three incubation strategies were used: (1) SCs proliferated at the control temperature of 38°C and differentiated at 43° or 33°C; (2) SCs proliferated at 43° or 33°C and differentiated at 38°C; or (3) SCs both proliferated and differentiated at 43°, 38°, or 33°C. During proliferation, lipid accumulation increased at 43°C and decreased at 33°C with the NC line showing greater variation than the RBC2 line. During proliferation at 43°C, peroxisome proliferator-activated receptor-γ (PPARγ) and neuropeptide-Y (NPY) expression was reduced to a greater extent in the NC line than the RBC2 line. At 33°C, expression of PPARγ, NPY, and CCAAT/enhancer-binding protein-β (C/EBPβ) was upregulated, but only in the RBC2 line. During differentiation, both lines showed greater changes in lipid accumulation and in C/EBPβ and NPY expression if the thermal challenge was initiated during proliferation. These data suggest that adipogenic gene expression is more responsive to thermal challenge in proliferating SCs than in differentiating SCs, and that growth-selection has increased temperature sensitivity of SCs, which may significantly affect breast muscle structure and composition.

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