Response of two mouse tumours to hyperthermia with CCNU or melphalan

Joiner, Michael C.; Steel, G. Gordon; Stephens, T C

doi:10.1038/bjc.1982.3

Cited by 45 publications

(9 citation statements)

References 9 publications

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“…The most commonly used method for inducing hyperthermia in murine tumor models is to submerse the tumor bearing portion of the mouse into a hot water bath (4-8). Since this method cannot provide selective power deposition into tissues at depth, it has been useful mainly for tumors in the hind limb where the region immersed can be limited primarily to the target tumor mass.…”

Section: Introductionmentioning

confidence: 99%

Miniature microwave applicator for murine bladder hyperthermia studies

Salahi

Maccarini

Rodrigues

et al. 2012

International Journal of Hyperthermia

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Purpose Novel combinations of heat with chemotherapeutic agents are often studied in murine tumor models. Currently, no device exists to selectively heat small tumors at depth in mice. In this project, we modelled, built and tested a miniature microwave heat applicator, the physical dimensions of which can be scaled to adjust the volume and depth of heating to focus on the tumor volume. Of particular interest is a device that can selectively heat murine bladder. Materials and Methods Using Avizo® segmentation software, we created a numerical mouse model based on micro-MRI scan data. The model was imported into HFSS™ simulation software and parametric studies were performed to optimize the dimensions of a water-loaded circular waveguide for selective power deposition inside a 0.15ml bladder. A working prototype was constructed operating at 2.45GHz. Heating performance was characterized by mapping fiber-optic temperature sensors along catheters inserted at depths of 0-1mm (subcutaneous), 2-3mm (vaginal), and 4-5mm (rectal) below the abdominal wall, with the mid-depth catheter adjacent to the bladder. Core temperature was monitored orally. Results Thermal measurements confirm the simulations which demonstrate that this applicator can provide local heating at depth in small animals. Measured temperatures in murine pelvis show well-localized bladder heating to 42-43°C while maintaining normothermic skin and core temperatures. Conclusions Simulation techniques facilitate the design optimization of microwave antennas for use in pre-clinical applications such as localized tumor heating in small animals. Laboratory measurements demonstrate the effectiveness of a new miniature water-coupled microwave applicator for localized heating of murine bladder.

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Section: Introductionmentioning

confidence: 99%

Miniature microwave applicator for murine bladder hyperthermia studies

Salahi

Maccarini

Rodrigues

et al. 2012

International Journal of Hyperthermia

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“…Cis-platinum was shown to be active against B16 melanoma in the Division of Cancer Treatment (National Cancer Institute) tumor panel [43]. Lower doses of melphalan than that applied in the present investigation (10mg/kg in single or 20mg/kg in two injections) inhibited the primary growth of LLC or B16M: 5 mg/kg or 8 mg/kg reduced the fraction of surviving cells in LLC or B16M to 10 per cent, respectively [14,29,35]. Lower doses of melphalan than that applied in the present investigation (10mg/kg in single or 20mg/kg in two injections) inhibited the primary growth of LLC or B16M: 5 mg/kg or 8 mg/kg reduced the fraction of surviving cells in LLC or B16M to 10 per cent, respectively [14,29,35].…”

Section: Discussionmentioning

confidence: 77%

The effectiveness of cis-platinum, cyclophosphamide and melphalan in treating disseminated tumor cells in mice

Kanclerz

Chapman

1987

Clin Exp Metast

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Both B16 melanoma and Lewis lung carcinoma growing in C57/Bl mice spontaneously metastasize to the lungs and other organs. When the tumors are grown in the mouse tail to a specific volume and amputated, the spontaneously disseminated tumor cells can then be independently treated. The effects of a single dose of cyclophosphamide (200 mg/kg), cis-platinum (6 mg/kg) and melphalan (10 mg/kg) on the appearance of pulmonary and other metastases were measured. The cis-platinum treatment was shown to reduce the number and incidence of metastases of both tumors at various times after treatment. The antimetastatic effectiveness of cis-platinum against these two tumors was increased when 2.4 mg/kg was administered each day for five consecutive days after amputation of the primary. Cyclophosphamide, when administered at two-thirds maximum tolerated dose, had a small promoting effect on the number and incidence of pulmonary metastases of Lewis lung carcinoma, whereas, applied in the same dose, it had efficacy in the treatment of disseminated B16 melanoma and inhibited appearance of both pulmonary and lymph-node metastases. When melphalan was administered in single- and multiple-dose regimens, the number and incidence of metastases of both tumors increased at various times after primary tumor amputation. These data suggest that melphalan can promote the growth of disseminated tumor cells in both the lungs and other sites and that some systemic chemotherapies may result in promotion instead of suppression of metastatic disease.

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“…IHP involves complete vascular isolation of the liver in order to maximize hepatic delivery of cytotoxic agents while minimizing systemic exposure and its inherent toxicities. In addition, hyperthermia can be a component of treatment and has been demonstrated in animal models to increase tumor vascular permeability . The operative technique has been described in detail in the literature elsewhere .…”

Section: Isolated Hepatic Perfusionmentioning

confidence: 99%

Isolated hepatic perfusion for metastatic melanoma

Yamamoto

Zager

2013

Journal of Surgical Oncology

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Unresectable hepatic metastases from melanoma present a challenge to the physician. Although there have been advances in systemic therapies for patients with metastatic cutaneous melanoma to the liver, the overall prognosis is poor and is worse in patients with metastatic ocular melanoma. Isolated hepatic perfusion, open or percutaneous, allows high doses of cytotoxic chemotherapy to be delivered directly to the liver while minimizing systemic exposure, and has emerged as a viable treatment options for these complex patients.

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Response of two mouse tumours to hyperthermia with CCNU or melphalan

Cited by 45 publications

References 9 publications

Miniature microwave applicator for murine bladder hyperthermia studies

Miniature microwave applicator for murine bladder hyperthermia studies

The effectiveness of cis-platinum, cyclophosphamide and melphalan in treating disseminated tumor cells in mice

Isolated hepatic perfusion for metastatic melanoma

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