Response of Wild Cucumis Species to Inoculation with Fusarium oxysporum f. sp. melonis Race 1,2y

Matsumoto, Yuichi; Ogawara, Takashi; Miyagi, Makoto; Watanabe, Naoharu; Kuboyama, Tetsuji

doi:10.2503/jjshs1.80.414

Cited by 29 publications

(21 citation statements)

References 24 publications

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“…Fom-1과 Fom-2 저항성 유전자는 단인자 우성 유전 을 한다고 알려져 있으며 (Zuniga et al, 1999), 현재 race 0, 1, 그리고 2에 저항성인 멜론 품종이 개발되어 있으나, race 1, 2에 대한 저항성인 멜론 품종은 아직 개발된 적이 없다 (Matsumoto et al, 2011 (Freeman and Rodriguez, 1993;Latin and Snell, 1986) (Thompson et al, 1994) 와 PHYDIT program (Chun, 1995)을 이용하여 분석하였다.…”

Section: Fusarium Oxysporum은 형태상으로는 구별이 되지 않지만unclassified

Development of Efficient Screening Methods for Melon Plants Resistant to Fusarium oxysporum f. sp. melonis

Lee¹,

Lee²,

Jang³

et al. 2015

HST

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This study was conducted to establish an efficient screening system to identify melon resistant to Fusarium oxysporum f. sp. melonis. F. oyxsporum f. sp. melonis GR was isolated from infected melon plants collected at Goryeong and identified as F. oxysporum f. sp. melonis based on morphological characteristics, molecular analyses, and host-specificity tests on cucurbits including melon, oriental melon, cucumber, and watermelon. In addition, the GR isolate was determined as race 1 based on resistance responses of melon differentials to the fungus. To select optimized medium for mass production of inoculum of F. oxysporum f. sp. melonis GR, six media were tested. The fungus produced the most spores (microconidia) in V8-juice broth. Resistance degrees to the GR isolate of 22 commercial melon cultivars and 6 rootstocks for melon plants were investigated. All tested rootstocks showed no symptoms of Fusarium wilt. Among the tested melon cultivars, only three cultivars were susceptible and the other cultivars displayed moderate to high resistance to the GR isolate. For further study, six melon cultivars (Redqueen, Summercool, Superseji, Asiapapaya, Eolukpapaya, and Asiahwanggeum) showing different degrees of resistance to the fungus were selected. The development of Fusarium wilt on the cultivars was tested according to several conditions such as plant growth stage, root wounding, dipping period of roots in spore suspension, inoculum concentration, and incubation temperature to develop the disease. On the basis of the test results, we suggest that an efficient screening method for melon plants resistant to F. oxysporum f. sp. melonis is to remove soil from roots of seven-day-old melon seedlings, to dip the seedlings without cutting in spore suspension of 3 × 10 5 conidia/mL for 30 min, to transplant the inoculated seedlings to plastic pots with horticulture nursery media, and then to cultivate the plants in a growth room at 25 to 28°C for about 3 weeks with 12-hour light per day.

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Section: Fusarium Oxysporum은 형태상으로는 구별이 되지 않지만unclassified

Development of Efficient Screening Methods for Melon Plants Resistant to Fusarium oxysporum f. sp. melonis

Lee¹,

Lee²,

Jang³

et al. 2015

HST

View full text Add to dashboard Cite

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“…, race (root-dipping method) (Cohen , 1989;Lee , 2015;Matsumoto , 2011;Namiki , 1998;Wellman, 1939). (Latin Snell, 1986 (Freeman , 2002;Iori , 2001;Zhou Everts, 2007;Zink , 1983), Risser , 1976) Seedlings of melon cultivars were uprooted and the roots were washed gently in water.…”

Section: Melonis(fom)mentioning

confidence: 99%

“…20-30 o C (Cohen , 1989;Matsumoto , 2011;Namiki , 1998;Zhou Everts, 2007;Zink , 1983 Seven-day-old seedlings of melon cultivars were inoculated with Fusarium oxysporum f. sp. melonis GR by cutting the roots with a scalpel and then pouring a 10 ml-aliquot of spore suspensions of 1×10 6 conidia/ml and 1×10 7 conidia/ml on soil.…”

mentioning

confidence: 99%

Development of an Efficient Simple Mass-Screening Method for Resistant Melon to Fusarium oxysporum f. sp. melonis

Lee¹,

Jang²,

Choi³

et al. 2015

Research in Plant Disease

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This study was conducted to establish a simple mass-screening method for resistant melon to Fusarium wilt caused by Fusarium oxysporum f. sp. melonis (FOM). Root-dipping inoculation method has been used to investigate resistance of melon plants to Fusarium wilt. However, the inoculation method requires a lot of labor and time because of complicate procedure. To develop a simple screening method on melon Fusarium wilt, occurrence of Fusarium wilt on susceptible and resistant cultivars of melon according to inoculation method including root-dipping, soil-drenching, tip, and scalpel methods was investigated. Scalpel and tip methods showed more clear resistant and susceptible responses in the melon cultivars than root-dipping inoculation method, but tip method represented slightly variable disease severity. In contrast, in the case of soil-drenching inoculation method, disease severity of the susceptible cultivars was very low. Thus we selected scalpel method as inoculation method of a simple screening method for melon Fusarium wilt. By using the scalpel inoculation method, resistance degrees of the cultivars according to incubation temperature after inoculation (25 and 30 o C) and inoculum concentration (1×10 6 and 1×10 7 conidia/ml) were measured. The resistance or susceptibility of the cultivars was hardly affected by all the tested conditions. To look into the effectiveness of scalpel inoculation methods, resistance of 22 commercial melon cultivars to FOM was compare with root-dipping inoculation method. When the melon cultivars were inoculated by scalpel method, resistance responses of all the tested cultivars were clearly distinguished as by root-dipping method. Taken together, we suggest that an efficient simple mass-screening method for resistant melon plant to Fusarium wilt is to sow the seeds of melon in a pot (70 ml of soil) and to grow the seedlings in a greenhouse (25±5 o C) for 7 days, to cut the root of seedlings with a scalpel and then pour a 10 ml-aliquot of the spore suspension of 1×10 6 conidia/ml on soil. The infected plants were cultivated in a growth room at 25 to 30 o C for about 3 weeks with 12-hr light a day.

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“…For the inoculation test, the fungal strains JCM 9889; Mel 02221; JCM 9288; and Fom 142-S1, which are classified as races 0; 1; 2; and 1,2, respectively (Matsumoto et al 2011, Namiki et al 1998, 2000, were used. Inoculation was performed using a root dip method (Matsumoto et al 2011). Each fungal strain was cultured in 100 mL of potato dextrose broth (PDB) in 300-mL flasks on a rotary shaker (ca.…”

Section: Evaluation Of Resistance To Fusarium Wiltmentioning

confidence: 99%

“…Fusarium strains are divided into 4 races (0, 1, 2, and 1,2) based on their pathogenicity on a set of differential genotypes. Races 0 and 2 were generally observed in Japan, while resistant cultivars are effective for controlling such soilborne diseases in melons (Matsumoto et al 2011, Namiki et al 1998.…”

Section: Introductionmentioning

confidence: 99%

Development of a New Melon Cultivar ‘Ibaraking’ with High Fruit Growth Ability under Low Temperature Conditions, High Total Soluble Solid Content, and Resistance to Fusarium Wilt

Matsumoto

Ishikawa

Miyagi

2014

JARQ

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Response of Wild Cucumis Species to Inoculation with Fusarium oxysporum f. sp. melonis Race 1,2y

Cited by 29 publications

References 24 publications

Development of Efficient Screening Methods for Melon Plants Resistant to Fusarium oxysporum f. sp. melonis

Development of Efficient Screening Methods for Melon Plants Resistant to Fusarium oxysporum f. sp. melonis

Development of an Efficient Simple Mass-Screening Method for Resistant Melon to Fusarium oxysporum f. sp. melonis

Development of a New Melon Cultivar ‘Ibaraking’ with High Fruit Growth Ability under Low Temperature Conditions, High Total Soluble Solid Content, and Resistance to Fusarium Wilt

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