Response properties of horizontal cells in the isolated retina of wild- type and pearl mutant mice

Suzuki, Hitoshi; Pinto, LH

doi:10.1523/jneurosci.06-04-01122.1986

Cited by 44 publications

(40 citation statements)

References 22 publications

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“…Although axon terminal recordings could not be distinguished from somatic recordings, axon terminals did show a higher sensitivity than somata (Fig. 6C), as has been reported previously (Suzuki and Pinto, 1986).…”

Section: Horizontal Cell Axon Terminals Receive Mixed Rod-cone Inputssupporting

confidence: 80%

Rod and Cone Contributions to Horizontal Cell Light Responses in the Mouse Retina

Trümpler¹,

Dedek²,

Schubert³

et al. 2008

Journal of Neuroscience

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Mammalian B-type horizontal cells make contact with both photoreceptor types: the dendrites contact cone photoreceptors, whereas the axon terminal processes contact rods. Despite their distinct synaptic contacts, horizontal cell somata and axon terminals receive a mixture of rod and cone inputs. Interaction of the two photoreceptor systems is essential for adaptation of photoreceptor sensitivity to different levels of background illumination, and horizontal cells play a key role in this adaptation. In this study, we used transgenic mouse lines to examine the contributions of rod and cone photoreceptor inputs to horizontal cell light responses in the mouse retina: rod signals were isolated by recording intracellularly from horizontal cells in a mouse lacking the cone cyclic nucleotide-gated channel, which lacks cone function, and cone signals were assessed using the rhodopsin knock-out mouse, which is a model for pure cone function. We found that both horizontal cell compartments receive a mixture of inputs from both photoreceptor types. To determine whether these inputs arrive via the long axon connecting the compartments or by way of rod-cone gap junctional coupling, we assessed the rod and cone contributions to horizontal cell somatic and axon terminal light responses in the connexin36-deficient mouse retina, which lacks rodcone coupling. Our results confirm that rods and cones are coupled by connexin36, and suggest that signal transmission along the axon is unidirectional: signals are passed from horizontal cell soma to axon terminal but not from axon terminal to soma.

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Section: Horizontal Cell Axon Terminals Receive Mixed Rod-cone Inputssupporting

confidence: 80%

Rod and Cone Contributions to Horizontal Cell Light Responses in the Mouse Retina

Trümpler¹,

Dedek²,

Schubert³

et al. 2008

Journal of Neuroscience

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“…While recording have been made from single cells in the isolated mouse retina (Balkema et al, 1981;Suzuki & Pinto, 1986;Stone & Pinto, 1993), we believe this to be the first report of ERG recordings which are similar in form to those recorded from the mouse retina in vivo (Fig. 1a).…”

Section: Discussionmentioning

confidence: 63%

Normal photoresponses and alteredb-wave responses to APB in themdx^Cv3mouse isolated retina ERG supports role for dystrophin in synaptic transmission

2004

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The mdx Cv3 mouse is a model for Duchenne muscular dystrophy (DMD). DMD is an X-linked disorder with defective expression of the protein dystrophin, and which is associated with a reduced b-wave and has other electroretinogram (ERG) abnormalities. To assess potential causes for the abnormalities, we recorded ERGs from pieces of isolated C57BL/6J and mdx Cv3 mouse retinas, including measurements of transretinal and intraretinal potentials. The ERGs from the isolated mdx Cv3 retina differ from those of control retinas in that they show reduced b-wave amplitudes and increased b-wave implicit times. Photovoltages obtained by recording across the photoreceptor outer segments of the retinas did not differ from normal, suggesting that the likely causes of the reduced b-wave are localized to the photoreceptor to ON-bipolar synapse. At a concentration of 50 μM, the glutamate analog DL-2-amino-4-phosphonobutyric acid (APB) blocks the b-wave component of the ERG, by binding to sites on the postsynaptic membrane. The On-bipolar cell contribution to the ERG was inferred by extracting the component that was blocked by APB. We found that this component was smaller in amplitude and had longer response latencies in the mdx Cv3 mice, but was of similar overall time course. To assess the sensitivity of sites on the postsynaptic membrane to glutamate, the concentration of APB in the media was systematically varied, and the magnitude of blockage of the light response was quantified. We found that the mdx Cv3 retina was 5-fold more sensitive to APB than control retinas. The ability of lower concentrations of APB to block the b-wave in mdx Cv3 suggests that the ERG abnormalities may reflect alterations in either glutamate release, the glutamate postsynaptic binding sites, or in other proteins that modulate glutamate function in ON-bipolar cells.

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“…The mouse has only one type of horizontal cell, of the axon-bearing B-type morphology (Suzuki and Pinto, 1986;Peichl and González-Soriano, 1994). Figure 4 A-C shows the localization of ZO-1 immunoreactivity on the dendrites of a Neurobiotin-injected horizontal cell network.…”

Section: Spatial Relationship Of Zo-1 To Cx50 and Cx57 On Horizontal mentioning

confidence: 99%

ZO-1 and the Spatial Organization of Gap Junctions and Glutamate Receptors in the Outer Plexiform Layer of the Mammalian Retina

Puller¹,

Müller²,

Janssen‐Bienhold³

et al. 2009

J. Neurosci.

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Information processing in the retina starts at the first synaptic layer, where photoreceptors and second-order neurons exhibit a complex architecture of glutamatergic and electrical synapses. To investigate the composition of this highly organized synaptic network, we determined the spatial relationship of zonula occludens-1 (ZO-1) with different connexins (Cx) and glutamate receptor (GluR) subunits in the outer plexiform layer (OPL) of rabbit, mouse, and monkey retinas. ZO-1 is well known as an intracellular component of tight and adherens junctions, but also interacts with various connexins at gap junctions. We found ZO-1 closely associated with Cx50 on dendrites of A-type horizontal cells in rabbit, and with Cx57 at dendro-dendritic gap junctions of mouse horizontal cells. The spatial arrangement of ZO-1 at the giant gap-junctional plaques in rabbit was particularly striking. ZO-1 formed a clear margin around the large Cx50 plaques instead of being colocalized with the connexin staining. Our finding suggests the involvement of ZO-1 in the composition of tight or adherens junctions around gap-junctional plaques instead of interacting with connexins directly. Furthermore, gap junctions were found to be clustered in close proximity to GluRs at the level of desmosome-like junctions, where horizontal cell dendrites converge before invaginating the cone pedicle. Based on this distinct spatial organization of gap junctions and GluRs, it is tempting to speculate that glutamate released from the photoreceptors may play a role in modulating the conductance of electrical synapses in the OPL.

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Response properties of horizontal cells in the isolated retina of wild- type and pearl mutant mice

Cited by 44 publications

References 22 publications

Rod and Cone Contributions to Horizontal Cell Light Responses in the Mouse Retina

Rod and Cone Contributions to Horizontal Cell Light Responses in the Mouse Retina

Normal photoresponses and alteredb-wave responses to APB in themdx^Cv3mouse isolated retina ERG supports role for dystrophin in synaptic transmission

ZO-1 and the Spatial Organization of Gap Junctions and Glutamate Receptors in the Outer Plexiform Layer of the Mammalian Retina

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Response properties of horizontal cells in the isolated retina of wild- type and pearl mutant mice

Cited by 44 publications

References 22 publications

Rod and Cone Contributions to Horizontal Cell Light Responses in the Mouse Retina

Rod and Cone Contributions to Horizontal Cell Light Responses in the Mouse Retina

Normal photoresponses and alteredb-wave responses to APB in themdxCv3mouse isolated retina ERG supports role for dystrophin in synaptic transmission

ZO-1 and the Spatial Organization of Gap Junctions and Glutamate Receptors in the Outer Plexiform Layer of the Mammalian Retina

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Normal photoresponses and alteredb-wave responses to APB in themdx^Cv3mouse isolated retina ERG supports role for dystrophin in synaptic transmission