Response surface method to optimize the low cost medium for protease production using anchovy meal from ascidian associated Bacillus sp. GA CAS10

Kumar, Ramamoorthy Sathish; Ananthan, G.; Iyappan, Kathirvel

doi:10.5897/ajb2014.13891

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…This indicates that the bacteria utilized fish gut waste substrate as the primary nutrient for its growth by producing protease enzyme. Positive relationship between growth of bacteria and protease activity has been previously reported 16 .…”

Section: Resultsmentioning

confidence: 68%

Production of thermotolerant, detergent stable alkaline protease using the gut waste of Sardinella longiceps as a substrate: Optimization and characterization

Ramkumar

Sivakumar

Gujarathi

et al. 2018

Sci Rep

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The gut wastes of Sardinella longiceps were used as substrate for protease production. The gut waste has 61.6% proteins, 21.8% lipids, 8.5% carbohydrates on dry weight basis and trace elements. The significant factors of protease fermentation were screened by Plackett-Burman design. A protease activity of 68.56 U/ml was predicted at 46.31 °C, incubation time 71.11 h, inoculum 4.86% (v/v) and substrate concentration 2.66% (w/v), using response surface methodology. However, the validation experiment showed 73.52 U/ml activity. The artificial neural network was found as a better tool to predict the experimental results. The partially purified protease showed higher activity at pH 9 and 10 and retained 90% activity after 120 h at pH 9. It showed maximum activity at 50 °C and retained 88% residual activity until 90 min at 50 °C. Zn++ enhanced the protease activity by 40%. The protease retained an activity of 93, 103, 90 and 98% against urea, β-mercaptoethanol, SDS and tween 80 respectively. The alkaline protease was compatible with all the commercial detergents tested with the residual activity above 90%. The alkaline protease exhibited 22% higher activity on the tryptone soya substrate. The gut waste of S. longiceps is a worthy low cost substrate for the production of industrially important alkaline protease.

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Section: Resultsmentioning

confidence: 68%

Production of thermotolerant, detergent stable alkaline protease using the gut waste of Sardinella longiceps as a substrate: Optimization and characterization

Ramkumar

Sivakumar

Gujarathi

et al. 2018

Sci Rep

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“…Nearly two-third of commercial proteases are produced by fungal species, yeasts and bacteria (1). Proteases (EC 3.4.21) are the significant class of industrially produced enzymes, which constitute around 60% of the total world enzyme production (2). Proteases are exploited in the food, dairy, leather, detergents and brewing industries (3).…”

Section: Introductionmentioning

confidence: 99%

Purification, Characterization and Thermodynamic Assessment of an Alkaline Protease by Geotrichum Candidum of Dairy Origin

et al. 2019

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Background Alkaline proteases is the important group of enzymes having numerous industrial applications including dairy food formulations. Objectives The current study deals with the purification and characterization of an alkaline serine protease produced by Geotrichum candidum QAUGC01, isolated from indigenous fermented milk product, Dahi. Material and Methods In total twelve G. candidum strains were screened for their proteolytic activity by using standard protease assay. The protease production from G. candidum QAUGC01 was optimized by varying physio-chemical conditions. The protease was purified by using two-step method: ammonium sulfate precipitation and gel filtration chromatography. Protease was further characterized by studying various parameter like temperature, pH, modulators, metal ions and organic solvent. A thermodynamic study was also carried out to explore the half-life of protease. Results The G. candidum grew profusely at 25 °C and at an initial pH of 4.0 for 72 h of incubation producing 26.21 U/ml maximum extracellular protease. Protease revealed that V max and K m was 26.25 U.ml -1 .min -1 and 0.05 mg.mL -1 , respectively using casein as substrate. The enzyme was stable at a temperature range (25–45 °C) and pH (8–9). Residual enzyme activity was strongly inhibited in the presence of PMSF (7.5%). The protease could hydrolyze proteinaceous substrates, casein (98%) and BSA (95%). The thermodynamic studies explored that the half-life of the enzyme that was 106.62 min, 38.72 min and 15.71 min at 50, 60 and 70 °C, respectively. Conclusions Purified protease from G. candidum GCQAU01 is an ideal candidate for industrial application.

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Biotechnological valorization of proteases: From hyperproduction to industrial exploitation—A review

Kamal

Rehman

Iqbal

2016

Env Prog and Sustain Energy

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From the last few years, due to the inadequacies of, in practice, physiochemical methods, growing scientific awareness, increasing ecological/environmental concerns, and legal boundaries, many industries are currently pursuing enzyme‐based approaches for developing green chemistry technologies. Proteases, responsible for proteolysis, are vitally important for life and engaged with vast industrial applications as they are eco‐friendly in nature. According to the bioinformatics, protease constitutes approximately 2% of the total human genome, whereas extracellular protease is a highly exploitable enzyme in various industries due to its robust nature, and commercially available with the trade name of Savinase, subtilisin Carlsberg, and subtilisin BPN′. Genetic modifications and immobilization revealed a novel protease production strategy with superior catalytic efficacy and improved constancy toward pH or temperature. A vast literature exists on biological activities of protease but only a few reports are available on the nutritional effects and the physiochemical parameters for fermentative production of protease, which offers new possibilities and potentials to fulfill the industrial demands of enzymes. This article focuses on the updated tidings on nutritional effects, physiochemical parameters, biochemical aspects, and strain improvement methodologies for hyperproduction of protease. This article also addresses existing challenges and tentative solutions for successful utilization of protease for industrial applications. © 2016 American Institute of Chemical Engineers Environ Prog, 36: 511–522, 2017

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Response surface method to optimize the low cost medium for protease production using anchovy meal from ascidian associated Bacillus sp. GA CAS10

Cited by 3 publications

References 16 publications

Production of thermotolerant, detergent stable alkaline protease using the gut waste of Sardinella longiceps as a substrate: Optimization and characterization

Production of thermotolerant, detergent stable alkaline protease using the gut waste of Sardinella longiceps as a substrate: Optimization and characterization

Purification, Characterization and Thermodynamic Assessment of an Alkaline Protease by Geotrichum Candidum of Dairy Origin

Biotechnological valorization of proteases: From hyperproduction to industrial exploitation—A review

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