Response surface methodology based extraction of Tribulus terrestris leads to an upsurge of antilithiatic potential by inhibition of calcium oxalate crystallization processes

Kaushik, Jyoti; Tandon, Simran; Gupta, Varun; Nayyar, Jasamrit; Singla, Sumit; Tandon, Chanderdeep

doi:10.1371/journal.pone.0183218

Cited by 13 publications

(17 citation statements)

References 34 publications

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“…terrestris were obtained from Natural Remedies Pvt.Ltd., Bangalore, India and voucher specimen is available with the company. Aqueous extract was prepared for oral dosing using methods described in our previous study 7 . Briefly, the dried and matured fruit of T .…”

Section: Methodsmentioning

confidence: 99%

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Delving into the Antiurolithiatic Potential of Tribulus terrestris Extract Through –In Vivo Efficacy and Preclinical Safety Investigations in Wistar Rats

Kaushik

Tandon

Bhardwaj

et al. 2019

Sci Rep

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Modern treatment interventions for kidney stones are wrought with side-effects, hence the need for alternative therapies such as plant-based medicines. We have previously documented through in vitro studies that statistically optimized aqueous extract of Tribulus terrestris (Zygophyllaceae family) possesses antiurolithic and antioxidant potential. This provides strong scientific foundation to conduct in vivo efficacy and preclinical safety studies to corroborate and lend further proof to its ability to prevent and cure kidney stones. The preventive and curative urolithiatic efficacy in experimentally induced nephrolithiatic Wistar rats, along with preclinical toxicity was evaluated following oral administration of statistically optimized aqueous extract of T. terrestris. Treatment showed augmented renal function, restoration of normal renal architecture and increase in body weight. Microscopic analysis of urine revealed excretion of small sized urinary crystals, demonstrating that treatment potentially modulated the morphology of renal stones. Tissue enzymatic estimation affirmed the antioxidant efficacy of treatment with reduced free radical generation. Significant upregulation of p38MAPK at both the gene and protein level was noted in hyperoxaluric group and interestingly treatment reversed it. Acute oral toxicity study established the Median Lethal Dose (LD50) to be greater than 2000 mg/kg body weight (b.wt.) No observed adverse effect level (NOAEL) by repeated oral toxicity for 28 days at 750 mg/kg b.wt. was noted. This study lends scientific evidence to the safe, preventive and curative potential of statistically optimized aqueous extract of T. terrestris at a dose of 750 mg/kg b.wt. and suggests that the extract shows promise as a therapeutic antiurolithic agent.

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Section: Methodsmentioning

confidence: 99%

“…Our preliminary in vitro studies provided the scientific basis and important insights into the anticalcifying potential of the statistically optimized aqueous extract of T . terrestris 7 . However, to extrapolate the results obtained in the cell lines to the dynamic animal model, in-depth in vivo studies are necessary.…”

Section: Introductionmentioning

confidence: 99%

Delving into the Antiurolithiatic Potential of Tribulus terrestris Extract Through –In Vivo Efficacy and Preclinical Safety Investigations in Wistar Rats

Kaushik

Tandon

Bhardwaj

et al. 2019

Sci Rep

Self Cite

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“…RSM has been successfully used and applied in many diverse areas such as biotechnology [44,45], chemical engineering [46,47], biofuel production [48], energy consumption [49], plant protection [50,51], and clinical practice [52,53]. One major contribution of this methodology is to investigate and quantify the relationships between a set of input variables and outputs of interest.…”

Section: Discussionmentioning

confidence: 99%

Ultrasonic-Assisted Pelleting of Sorghum Stalk: Predictive Models for Pellet Density and Durability Using Multiple Response Surface Methodology

Zhang

Shi

Zhang³

et al. 2018

Energies

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In the field of renewable energy, feedstock such as cellulosic biomass has been proposed as a renewable source of fuel to produce energy. However, the use of raw biomass as feedstock causes high costs in handling, transportation, and storage. Compressing raw cellulosic biomass into pellets significantly increases the density and durability of cellulosic biomass, reducing the transportation and handling costs of feedstock. To ensure high pellet quality, high pellet density and durability are desired during a compressing process. In this study, ultrasonic vibration-assisted (UV-A) pelleting, as a novel pelleting method, was applied to measure pellet density and durability during experiments. Response surface methodology (RSM) was employed to investigate the effects of pelleting time, ultrasonic power, and pelleting pressure on the pellet density and pellet durability. The model was validated by comparing the predictive results with experimental data and demonstrated a good predictive ability (R 2 > 0.95). By employing a Derringer and Suich's desirability function, our results suggest that the optimal pellet density and durability are 1239 kg/m 3 and 93%, respectively, when the pelleting time was set to 44 s, the ultrasonic power was set to 50%, and pressure was set to 42 psi (289,580 Pa).

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“…TT is used for kidney disorders by removing the gravel from the urine and stone in the bladder. [678910]…”

Section: Introductionmentioning

confidence: 99%

“…It has already been proven that TT plant extract has antioxidative, apoptosis inhibitory, and vasodilator properties, as well as protecting renal epithelial cells against damages due to oxalates and ethylene glycol consumption. [78910] The aim of this study was to investigate the protective effects of TT extracts against acute kidney injury (AKI) induced by mercuric chloride.…”

Section: Introductionmentioning

confidence: 99%

Effect of Tribulus terrestris in mercuric chloride-induced renal accumulation of mercury and nephrotoxicity in rat

Yadav

Sharma

Singh

et al. 2019

J Adv Pharm Technol Res

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Mercury generates free radicals and subsequently increases oxidative stress, which leads to renal injury. Tribulus terrestris (TT) has good anti-inflammatory and antioxidant properties. Hydroalcoholic extract of different dose of TT was evaluated against mercuric chloride-induced nephrotoxicity. Rats ( n = 6) were treated with TT at doses of 100, 200, and 300 mg/kg. Drugs were administered orally for 7 days. Single dose of mercuric chloride (5 mg/kg, intraperitoneal) on the 5 th day caused significant elevation of blood urea nitrogen, serum creatinine, malondialdehyde, liver fatty acid binding protein, kidney injury molecule-1, and kidney mercury level and fall in glutathione, superoxide dismutase, glutathione peroxidase, and histopathological changes in disease control as compared to normal control group ( P < 0.001). Dose of TT 200 and 300 mg/kg significantly ( P < 0.001) prevented the renal injury, and mercury accumulation in kidney tissues significantly decreases in higher dose, i.e., 300 mg/kg as compared to control group. Our result indicates that the treatment of TT exerted significant protection against renal damage induced by mercuric chloride possibly due to its antioxidant and anti-inflammatory properties and by decreasing the renal accumulation of mercury.

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Response surface methodology based extraction of Tribulus terrestris leads to an upsurge of antilithiatic potential by inhibition of calcium oxalate crystallization processes

Cited by 13 publications

References 34 publications

Delving into the Antiurolithiatic Potential of Tribulus terrestris Extract Through –In Vivo Efficacy and Preclinical Safety Investigations in Wistar Rats

Delving into the Antiurolithiatic Potential of Tribulus terrestris Extract Through –In Vivo Efficacy and Preclinical Safety Investigations in Wistar Rats

Ultrasonic-Assisted Pelleting of Sorghum Stalk: Predictive Models for Pellet Density and Durability Using Multiple Response Surface Methodology

Effect of Tribulus terrestris in mercuric chloride-induced renal accumulation of mercury and nephrotoxicity in rat

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